Cdh18 (NM_001081299) Mouse Tagged ORF Clone

CAT#: MG212860

Cdh18 (tGFP-tagged) - Mouse cadherin 18 (Cdh18), (10ug)

Product Images

Circular map for MG212860

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Cdh18
• Synonyms: B230220E17Rik; Cdh14l
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG212860 representing NM_001081299
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGAATTACGAGCACATCTTGCATCTGTCCAGTCCTCGTGTGTCTCTGTTTTGTGCAGAGGTGTTATG
  GAACTGCTCACCACAGCTCCATTAAGGTGACCAGAAACCAAACCAAACACACTGAAGGGGAAACTGAAGT
  ACACCATCGTCCCAAGAGGGGATGGGTATGGAACCAGTTCTTTGTTTTAGAAGAACATATGGGACCAGAC
  CCACAGTATGTTGGAAAACTGCACTCCAACTCCGACAAAGGTGATGGTTCTGTCAAGTACATCCTGACTG
  GAGAAGGGGCCGGGACTATATTTATCATTGATGACACCACAGGTGACATCCACTCAACCAAAAGCCTGGA
  CAGAGAGCAGAAGACTCACTATGTGCTCCATGCCCAGGCTATTGACAGACGGACAAACAAGCCTCTTGAA
  CCTGAGTCTGAGTTCATCATCAAAGTGCAAGATATTAATGACAATGCCCCAAAGTTCACAGATGGACCAT
  ACATCGTTACTGTGCCTGAAATGTCAGATATGGGTACCTCTGTTCTGCAGGTGACAGCTACAGATGCAGA
  TGACCCCACCTATGGGAACAGTGCTCGAGTTGTTTACAGTATCCTGCAAGGACAACCTTATTTCTCTGTA
  GATCCTAAAACAGGAGTCATTCGAACAGCTTTACACAACATGGATAGAGAAGCCAGAGAGCATTACTCAG
  TGGTCATTCAAGCCAAAGACATGGCTGGGCAAGTTGGCGGACTTTCTGGATCCACCACTGTCAATATTAC
  CTTGACTGATGTCAATGACAACCCACCACGGTTCCCACAGAAACACTATCAACTGTATGTTCCTGAGTCT
  GCTCAAGTTGGTTCAGCTGTTGGGAAAATCAAAGCAAATGATGCAGACACAGGATCAAATGCAGATATGA
  CCTACTCCATCACTAATGGAGACGGGATTGGAGTGTTCTCTATCTCCACGGACAAGGACACCAGAGAAGG
  AATACTTTCCTTAAAAAAGCCACTGAACTATGAGAAAAAGAAGTCTTACACACTCAACATTGAAGGAGCA
  AACACACATCTGGATTTCCGTTTTTCCCACTTGGGTCCATTTAAGGATGCCACCATGCTGAAGGTTATTG
  TTGGGGATGTGGATGAGCCACCACTCTTTTCTATGCCTTCCTATGTCATGGAAGTCTATGAAAATGCCAA
  GATTGGGACAATTGTTGGCACTGTCTTGGCTCAGGATCCAGACAGTGCTAACAGCTTAGTAAGGTATTTC
  ATCAATCACAGTACTGAAGAGGAGAGATTTTTCAATATTGATGCCAATACTGGAACCATTAAGACATCAA
  AAGTACTGGATAGAGAAGAAACACCATGGTACAATATCACAGTGACTGCTTCAGAAAACGACAATCCTGA
  TTTGCTGAGCCATGTCTCCGTGGGTATTAGAGTTCTGGATGTCAATGACAATCCACCTGAACTTGCCAGG
  GAATATGATATTGTTGTCTGTGAGAATTCTAAGCCTGGTCAGGTTATCCACACCATCACTGCCACTGATA
  AAGATGACTTTGCCAATGGACCGAGATTTCATTTTTTTCTTGATGAGCGCCTGCCCATGAACCCAAATTT
  CACACTTAAGGACAATGAAGATAACACAGCCAGCATTCTGACAAGGCGGAGGAGATTTAGTCGAACTATG
  CAGGATGTGTATTATCTGCCCATTATGATCTCTGACGGTGGAATCCCCTCTCTCAGCAGCAGCAGCACCC
  TCACCATCAGGGTTTGTGCATGCGAGAGAGATGGGCGCGTGCGGACCTGCCATGCAGAAGCCTTCCTGTC
  CTCCGCTGGTTTGAGCACAGGAGCCCTAATCGCTATTCTTCTCTGTGTTGTCATTCTTCTTGCCATTGTG
  GTCCTTTTTATCACCTTGAGGCGTGGCAAAAAAGAGCCCTTGATCATTTCCGAGGAGGATGTGCGAGAGA
  ATGTGGTGACATATGATGACGAGGGAGGAGGGGAGGAAGACACCGAAGCCTTCGACATCACAGCTTTGCG
  GAATCCTGCCGCAGCAGAGGAGTTCACGTACCGCAGAGACATTCGGCCTGAAGTGAAGCTCACTCCGAGA
  CATCAGACCTTGTCCACCCTGGAGAGCATAGACGTTCAGGAGTTTATTAAGCAAAGACTCGCAGAAGCCG
  ACCTGGACCCCAGCGTGCCCCCTTATGACTCTCTTCAGACTTATGCCTACGAGGGCCAGCGGTCAGAAGC
  TGGGTCTATCAGCTCACTGGACTCCGCAACCACGCAATCAGACCAGGATTATCACTACCTTGGAGACTGG
  GGGCCCGAGTTTAAGAAGTTAGCTGAACTCTATGGAGAAATAGAATCTGAAAGAACAACT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG212860 representing NM_001081299
  Red=Cloning site Green=Tags(s)
  
  MRITSTSCICPVLVCLCFVQRCYGTAHHSSIKVTRNQTKHTEGETEVHHRPKRGWVWNQFFVLEEHMGPD
  PQYVGKLHSNSDKGDGSVKYILTGEGAGTIFIIDDTTGDIHSTKSLDREQKTHYVLHAQAIDRRTNKPLE
  PESEFIIKVQDINDNAPKFTDGPYIVTVPEMSDMGTSVLQVTATDADDPTYGNSARVVYSILQGQPYFSV
  DPKTGVIRTALHNMDREAREHYSVVIQAKDMAGQVGGLSGSTTVNITLTDVNDNPPRFPQKHYQLYVPES
  AQVGSAVGKIKANDADTGSNADMTYSITNGDGIGVFSISTDKDTREGILSLKKPLNYEKKKSYTLNIEGA
  NTHLDFRFSHLGPFKDATMLKVIVGDVDEPPLFSMPSYVMEVYENAKIGTIVGTVLAQDPDSANSLVRYF
  INHSTEEERFFNIDANTGTIKTSKVLDREETPWYNITVTASENDNPDLLSHVSVGIRVLDVNDNPPELAR
  EYDIVVCENSKPGQVIHTITATDKDDFANGPRFHFFLDERLPMNPNFTLKDNEDNTASILTRRRRFSRTM
  QDVYYLPIMISDGGIPSLSSSSTLTIRVCACERDGRVRTCHAEAFLSSAGLSTGALIAILLCVVILLAIV
  VLFITLRRGKKEPLIISEEDVRENVVTYDDEGGGEEDTEAFDITALRNPAAAEEFTYRRDIRPEVKLTPR
  HQTLSTLESIDVQEFIKQRLAEADLDPSVPPYDSLQTYAYEGQRSEAGSISSLDSATTQSDQDYHYLGDW
  GPEFKKLAELYGEIESERTT
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001081299
• ORF Size: 2370 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001081299.1, NP_001074768.1
• RefSeq Size: 2744 bp
• RefSeq ORF: 2373 bp
• Locus ID: 320865
• Cytogenetics: 15 A2