Nap1l4 (NM_008672) Mouse Tagged ORF Clone

CAT#: MG212438

Nap1l4 (tGFP-tagged) - Mouse nucleosome assembly protein 1-like 4 (Nap1l4), (10ug)

Product Images

Circular map for MG212438

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Nap1l4
• Synonyms: 2810410H14Rik; AI316776; D7Wsu30e; Nap2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG212438 representing NM_008672
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGAAAACAGTCTTTCAGATGGAGGCCCTGCAGATTCTGTGGAAGCTGCTAAAAATGCCAGTAATA
  CAGAAAAACTCACAGACCAGGTGATGCAGAACCCACAAGTGCTGGCAGCTTTGCAGGAACGTCTTGACAA
  CGTCTCTCACACTCCTTCTAGTTACATAGAAACTTTACCTAAAGCTGTCAAAAGAAGAATTAATGCCCTG
  AAGCAGCTCCAGGTGAGGTGTGCGCACATAGAAGCTAAGTTCTACGAGGAAGTTCATGACCTGGAGAGGA
  AGTATGCAGCCTTGTACCAGCCTCTATTTGACAAGAGAAGAGAATTCATCACTGGTGACGTGGAGCCCAC
  AGATGCAGAGTCAGCGTGGCACAGTGAGAATGAGGAGGAGGACAAGTTGGCTGGAGATATGAAGAATAAA
  GTAGTCATAGCTGAGAAAGAAGCAGCAACAGTGGAAGAGCTGAACCCCAAAGGCATCCCTGAGTTCTGGT
  TCACCATCTTCAGAAATGTGGACATGCTTAGTGAGCTGGTGCAGGAATATGATGAACCGATCTTGAAACA
  TCTGCAGGATATTAAAGTGAAGTTTTCAGACCCTGGACAACCTATGTCTTTTGTGCTAGAGTTTCACTTT
  GAACCCAACGACTACTTCACCAATCCTGTCCTGACGAAAACGTACAAGATGAAATCAGAGCCAGACAAGG
  CCGACCCGTTTTCTTTTGAAGGTCCTGAAATTGTGGACTGTGATGGGTGTACAATAGACTGGAAGAAAGG
  AAAAAATGTCACAGTCAAAACCATCAAGAAAAAGCAGAAGCACAAGGGCCGGGGCACCGTACGAACCATC
  ACCAAGCAAGTGCCCAATGAGTCCTTCTTCAACTTCTTCAGCCCCCTGAAAGCCTCTGGAGATGGAGAAT
  CTCTGGATGAAGATTCCGAGTTCACCTTAGCCTCTGACTTTGAAATTGGGCACTTTTTTCGTGAGCGCAT
  CGTGCCACGGGCTGTCCTCTACTTCACTGGGGAGGCCATAGAGGATGATGACAATTTTGAAGAAGGCGAG
  GAAGGTGAAGAGGAGGAATTGGAAGGTGATGAAGAAGGAGAAGATGAGGATGATGCTGATGTTAACCCCA
  AGGTG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG212438 representing NM_008672
  Red=Cloning site Green=Tags(s)
  
  MAENSLSDGGPADSVEAAKNASNTEKLTDQVMQNPQVLAALQERLDNVSHTPSSYIETLPKAVKRRINAL
  KQLQVRCAHIEAKFYEEVHDLERKYAALYQPLFDKRREFITGDVEPTDAESAWHSENEEEDKLAGDMKNK
  VVIAEKEAATVEELNPKGIPEFWFTIFRNVDMLSELVQEYDEPILKHLQDIKVKFSDPGQPMSFVLEFHF
  EPNDYFTNPVLTKTYKMKSEPDKADPFSFEGPEIVDCDGCTIDWKKGKNVTVKTIKKKQKHKGRGTVRTI
  TKQVPNESFFNFFSPLKASGDGESLDEDSEFTLASDFEIGHFFRERIVPRAVLYFTGEAIEDDDNFEEGE
  EGEEEELEGDEEGEDEDDADVNPKV
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_008672
• ORF Size: 1125 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_008672.3, NP_032698.1
• RefSeq Size: 2283 bp
• RefSeq ORF: 1128 bp
• Locus ID: 17955
• UniProt ID: Q78ZA7
• Cytogenetics: 7 88.24 cM
• Gene Summary: Acts as histone chaperone in nucleosome assembly (PubMed:28366643). In condensing spermatids, mediates the loading of the heterodimer composed of histones H2AFB1 and HIST1H2BA/TH2B onto the nucleosomes, thereby promoting the replacement of histones to protamine in male germ cells (PubMed:28366643).[UniProtKB/Swiss-Prot Function]