Arhgap21 (BC076629) Mouse Tagged ORF Clone

CAT#: MG212100

Arhgap21 (tGFP-tagged) - Mouse Rho GTPase activating protein 21 (cDNA clone MGC:100159 IMAGE:30639338)

Product Images

Circular map for MG212100

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Arhgap21
• Synonyms: 5530401C11Rik; AA416458; ARHGAP10
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG212100 representing BC076629
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCACACATTGGACTGGTCTGCCTGAGGAAGATGGTGACAAACTCAAGGCCTGTGGGGCGGCCTCTG
  CCTGTGAGGTGTCAAAAAACAAAGATGGAAAAGACCAAGGTGAACCTGTGTCACCGTCTGAAGATGAGCC
  ATTCTCCTGGCCAGGTCCCAAAACAGTCATGCTGAAGAGAACATCTCAAGGGTTTGGTTTCACATTACGA
  CACTTCATCGTTTATCCCCCAGAGTCTGCAATCCAGTTTTCATATAAGGATGAAGAGAATGGAAACAGAG
  GAGGAAAGCAAAGGAACCGATTGGAGCCGATGGATACCATATTTGTTAAACAAGTGAAGGAAGGAGGGCC
  GGCCTTCGAGGCTGGCTTATGCACAGGTGACAGAATTATAAAAGTCAATGGAGAAAGTGTCATAGGAAAG
  ACCTATTCCCAAGTAATTGCTCTCATTCAGAACAGTGACACCACCCTGGAGCTCAGTGTAATGCCCAAGG
  ATGAAGACATTCTCCAAGTGGCATATTCTCAAGATGCATACCTGAAAGGCAATGAAGCTTACAGCGGCAA
  TGCCCGTAATATACCTGAACCTCCACCAGTTTGCTATCCCTGGTTGCCATCGACTCCTTCAGCTACTGCA
  CAGCCGGTGGAAACATGCCCTCCTGATTCATTACCGAACAAACAGCAAACCAGTGCACCAGTCCTCACAC
  AACCTGGCAGGGCCTATAGAATGGAAATACAAGTGCCTCCGTCACCAACAGATGTTGCAAAGTCAAACAC
  AGCAGTGTGTGTTTGCAATGAGAGTGTGAGGACCGTCATTGTGCCTTCTGAGAAGGTGGTAGACTTGCTA
  GCCAATAGAAACAACCCTTCAGGTCCTTCACATAGAACTGAAGAGGTAAGGTATGGTGTAAATGAGCAGG
  CCTCTACAAAAGCAGCATCGAGAACAACGTCACCGGCATCAGTTCCCACTGCCCATCTGATTCATCAGAC
  CACAGGCTCCAGATCCTTAGAACCTTCTGGAATTTTACTTAAATCTGGAAATTACAGTGGACATTCAGAA
  GGAATCTCAAGCAGTAGATCACAAGCCGTGGATTCCCCTCCTGTGTCTGTCAATCACTACTCTGCAAACT
  CCCATCAGCACATAGACTGGAAAAATTATAAGACTTACAAAGAATACATTGATAATAGGAGATTGCACAT
  AGGTTGTCGGACAATCCAGGAAAGACTGGACAGCTTACGAGCTGCATCTCAGAGCGCAGCAGACTATAAC
  CAGGTGGTACCCACCCGCACTACTCTGCAGGTACGACGTCGAAGCACCTCTCATGACCGAGTGCCCCAGT
  CTGTTCAGATCCGGCAGCGCAGTGTATCTCAGGAAAGACTGGAAGATTCTGTGCTGATGAAGTATTGCCC
  AAGGAGCGCGTCTCAAGGAGCGCTGACCTCACCGCCTGTTAGCTTCAATAATCATAGAACTCGTTCGTGG
  GATTACATAGAGGGACAGACGGAAGCCACAGCCACTGTCAATTCTGAAAGTCAGATACCTGACTCAAATG
  GGGAACGAAAACAGACTTACAAGTGGAGTGGCTTCACTGAGCAGGATGATAGACGCGGTATTCACGAAAG
  ACCCAGGCAGCAAGAAATGCACAAGCCTTTCCGAGGCTCAAATTTGACTGTGGCTCCCGTTGTTAATTCT
  GATAACAGGCGCCTGGTTGGGAGAGGGGTAGGACCTGTCTCACAGTTTAAAAAAATCCCGCCGGACCTAA
  GACCACCACATTCCAACAGAAATTTTCCAACCACGACTGGGGTGTCACTTCAACGGGGTATTGCACAAGA
  CAGGTCCCCACTCGTGAAAGTCCGAAGTAATTCTCTGAAAGTGCCACCTCCACCTGTGTCAAAACCGTCC
  TTCAGCCAGCACTCACTGGCTTCCATGAAAGACCAAAGGCCTGTCAATCATCTGCATCAACACAGTGTGC
  TGAGTCAGCAGACGCAGTTCAGATCCGAAAGCACCTTCGAGCACCAGCTGGAGACTGAGGTATCCTCGTG
  TCTACCAGGAACTTCTGCCAAGACCAGTCCACAGCTCAGTGAGAATTTGGGAACATCAGACTTAGAACTA
  CCTGCCATCCCAAGGAATGGAGATATAAACTTACAGGAGGCTGAAATTCAGCAGCCAGATGTGCTAGATA
  ATAAAGAATCTGTCATTCTAAGAGAGAAACCTCAGTCTGGCCGCCAGACGCCACAGCCTTTAAGACATCA
  GTCTTACATCCTGGCAGTGAATGACCAGGAGACCGGGTCAGACACCACCTGCTGGCTGCCAAACGATGCA
  CGCAGAGAGGTCCATATAAAAAGAATGGAGGAGAGGAAAGCTTCAAGCACCAGCCCTCCTGGTGATTCCC
  TGGCATCCATCCCTTTTATAGATGAGCCAACCAGTCCTAGCATCGACCACGAGATTGCACATATTCCTGC
  ATCTGCTGTCATCTCCGCCTCTACTGCCCACGTCCCCTCCATTGCAACTGTTCCCCCCAGCCTCACGACC
  TCTGCTCCTTTGATTCGACGTCAGCTCTCCCACGACCAAGAATCTGTTGGACCTCCCAGCCTGGATGGCC
  AGCACAGCTCAAAGACAGAAAGATCCAAATCCTACGATGAAGGACTGGATGATTATAGAGAAGATGCAAA
  ACTATCCTTTAAGCACGTGTCTAGCCTGAAGGGAATCAAGATTACAGACAGCCAGAAGTCATCAGAAGAC
  TCTGGATCGAGAAAGGGTTCTTCCTCCGAGGTCTTCAGTGATGCAGCCCGAGAAGGCTGGCTCCAGTTCC
  GACCACTGGTCACCGATAAGGGCAAGAGAGTTGGTGGCAGTATTCGGCCATGGAAACAGATGTATGTTGT
  CCTTCGGGGCCACTCACTTTACTTGTACAAAGACAGAAGAGAGCAGACAACGCCATCAGAAGAAGAGCAG
  CCCATCAGTGTCAACGCTTGCCTGATAGACATCTCGTACAGTGAGACCAAGAGGCGGAATGTATTCCGAC
  TTACCACGTCTGACTGTGAATGCTTGTTCCAGGCTGAAGACAGAGATGATATGTTATCATGGATCAAGAC
  TATTCAGGAAAGTAGCAACCTAAATGAAGAGGACACTGGAGTTACCAACAGAGATCTAATTAGTCGAAGA
  ATCAAAGAGTATAACAGTCTGCTGAGCAAAACAGAACAGTTGCCAAAAACACCTCGCCAGAGTCTTAGCA
  TCAGGCAAACTTTGCTTGGTGCTAAATCAGAGCCAAAGACTCAAAGCCCACATTCTCCAAAGGAGGAGTC
  CGAGAGGAAGCTTCTCAGTAAAGATGATACCAGTCCCCCTAAAGACAAAGGTACTTGGAGAAGAGGCATT
  CCAAGCATCGTGAGGAAGACGTTTGAGAAAAAGCCGGCCGCCACAGGGACCTTTGGAGTTAGGCTGGATG
  ACTGCCCACCCGCCCACACCAACAGGTACATTCCATTAATCGTTGACATATGTTGCAAATTAGTTGAAGA
  AAGGGGTCTTGAATATACAGGTATTTACCGAGTTCCCGGCAACAACGCAGCCATTTCAAGCATGCAGGAA
  GAACTCAACAAGGGGATGGCAGACATTGACATACAAGATGATAAATGGCGAGATTTGAATGTGATAAGCA
  GTTTACTAAAATCCTTCTTCAGAAAGCTCCCTGAGCCTCTCTTTACGAATGACAAATATGCTGACTTCAT
  TGAGGCCAATCGCAAAGAGGACCCACTGGATCGGCTACGAACATTAAAGAGACTAATTCATGACCTGCCT
  GAGCATCATTTTGAGACGCTCAAGTTTCTCTCGGCTCATCTGAAGACAGTGGCAGAGAACTCAGAAAAGA
  ATAAGATGGAACCCAGAAACCTAGCGATCGTTTTTGGTCCAACTCTAGTGAGAACATCAGAAGACAACAT
  GACCCACATGGTCACGCACATGCCTGACCAGTATAAGATCGTAGAGACTCTCATCCAGCATCATGACTGG
  TTTTTCACAGAAGAAGGTGCCGAAGAGCCTCTTACAGCAGTGCAGGAGGAGAACACAGTAGACTCCCAGC
  CAGTACCAAACATAGATCATTTGCTCACCAACATTGGAAGGACAGGCGTGCTCCCTGGAGATGTATCAGA
  TTCAGCTACTAGTGACTCCGCAAAATCTAAGGGCTCCTGGGGATCCGGGAAGGATCAGTATAGCAGGGAA
  CTGCTTGTATCCTCCATCTTCGCAGCTGCCAGTCGCAAGAGGAAAAAGCCGAAAGAGAAAGCCCAGCCCA
  GCAGCTCTGAGGATGAGCTGGACAGTGTGTTCTTTAAGAAGGAGAATACGGAACAGAGTCACAGTGAGAT
  TAAAGAAGAGTCCAAAAGAGAAAGCGAGACATCAGGTAGCAAGCAGAGGGTCGTCGTTGCCAAAGAAAGT
  AACACTAAGAAAGACTCGGGTACAACGAAGGAGGAGAAGAAGATACCGTGGGAAGAACCACCCCCACCAC
  ACAGCTCCAAACGCAACAGGTCACCAACCCTCAGCTGTCGCCTGGCCATGCTGAAAGAGGGCCCCAGATC
  ACTTCTGACACAGAAGCCCCACTGTGAAGAGACAGGGTCTGACTCAGGCACTCTGCTTAGCACGTCTTCC
  CAGGCTTCGCTCTTGAGGTCCTCCACTAAAAAATCCACCAGTCCGGAAACCAAGCACAGTGAGTTTTTGT
  CCATTGCTGGTACCACCACCTCGGATTATTCTACCACATCATCCACTACCTACTTGACCAGCCTGGACTC
  CAGCCGCCTGAGCCCTGAGGTGCAGTCAGTGGCCGAGAGCAAGGGGGATGAGGCTGATGACGAGAGGAGC
  GAGCTGGTGAGTGAAGGACGGCCAGTGGAGACCGACAGTGAGAGTGAGTTCCCCGTGTTTCCCACCACCC
  TGACTTCAGACCGGCTGTTCCGAGGAAAATTCCAGGAGGTTGCCAGGGTGAGCCGCAGGAACTCCGAAGG
  CAGTGAGGCCAGCTGCACCGAGGGAAGCCTCACACCGAGTTTAGACAGCCGCAGGCAGCAGTTCAGCTCC
  CATAGACTGATCGAATGCGACACACTGTCCAGGAAGAAGTCTGCTCGCTTCAAGTCAGACAGTGGGAGCC
  CCGGAGACACCAGGACTGAGAAAGAAACTCCTGCCCTGGCCAAGATGTTTGACGTCATGAAGAAAGGCAA
  GTCGACAGGGAGCCTCCTCACACCCAGCAGAAGTGAATCCGAAAAGCAGGAGGCCACTTGGAAAACAAAA
  ATAGCAGATCGTTTAAAACTGAGACCCAGGGCACCCGCAGACGACATGTTTGGAGTTGGGAATCAAAAAC
  CTACTGCTGAGACAGCGAAAAGGAAAAACATCAAACGCAGACACACGCTTGGAGGCCACAGAGATGCTAC
  TGAGATCAGCGTCCTGAGTTTCTGGAAAGCTCATGAGCAGAGTGCGGACAAAGAGTCTGAACTTTCGGCT
  GTGAATCGGTTAAAACCCAAATGCTCAGCCCAGGACCTTTCCATCTCAGACTGGCTCGCCAGGGAGCGCG
  TCCGCACCAGTGCCTCCGACCTTAGCAGAGGCGAAGGCCTCGAGCCCCAGGCTGAGAGCCCCAGCGTGCT
  GGGAACACCCATCAGCACCCACTCACCACCGTCTCAGCAGCCCGAAGCAAGGGTTGCTGCTACCAGCACC
  TTGGCTTCAACCAGCCAGTCCCCTCTTTTCACACCACCACAGTCACCTGACCAAATAAACAGAGAAAGCT
  TCCAGAACATGAGCCAAAATGCTAGTTCTACAGCTAACATCCACCCCCACAAACAGTCTGAAAGTCCAGA
  CACTAAAGCAGAGACTCCTCCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG212100 representing BC076629
  Red=Cloning site Green=Tags(s)
  
  MATHWTGLPEEDGDKLKACGAASACEVSKNKDGKDQGEPVSPSEDEPFSWPGPKTVMLKRTSQGFGFTLR
  HFIVYPPESAIQFSYKDEENGNRGGKQRNRLEPMDTIFVKQVKEGGPAFEAGLCTGDRIIKVNGESVIGK
  TYSQVIALIQNSDTTLELSVMPKDEDILQVAYSQDAYLKGNEAYSGNARNIPEPPPVCYPWLPSTPSATA
  QPVETCPPDSLPNKQQTSAPVLTQPGRAYRMEIQVPPSPTDVAKSNTAVCVCNESVRTVIVPSEKVVDLL
  ANRNNPSGPSHRTEEVRYGVNEQASTKAASRTTSPASVPTAHLIHQTTGSRSLEPSGILLKSGNYSGHSE
  GISSSRSQAVDSPPVSVNHYSANSHQHIDWKNYKTYKEYIDNRRLHIGCRTIQERLDSLRAASQSAADYN
  QVVPTRTTLQVRRRSTSHDRVPQSVQIRQRSVSQERLEDSVLMKYCPRSASQGALTSPPVSFNNHRTRSW
  DYIEGQTEATATVNSESQIPDSNGERKQTYKWSGFTEQDDRRGIHERPRQQEMHKPFRGSNLTVAPVVNS
  DNRRLVGRGVGPVSQFKKIPPDLRPPHSNRNFPTTTGVSLQRGIAQDRSPLVKVRSNSLKVPPPPVSKPS
  FSQHSLASMKDQRPVNHLHQHSVLSQQTQFRSESTFEHQLETEVSSCLPGTSAKTSPQLSENLGTSDLEL
  PAIPRNGDINLQEAEIQQPDVLDNKESVILREKPQSGRQTPQPLRHQSYILAVNDQETGSDTTCWLPNDA
  RREVHIKRMEERKASSTSPPGDSLASIPFIDEPTSPSIDHEIAHIPASAVISASTAHVPSIATVPPSLTT
  SAPLIRRQLSHDQESVGPPSLDGQHSSKTERSKSYDEGLDDYREDAKLSFKHVSSLKGIKITDSQKSSED
  SGSRKGSSSEVFSDAAREGWLQFRPLVTDKGKRVGGSIRPWKQMYVVLRGHSLYLYKDRREQTTPSEEEQ
  PISVNACLIDISYSETKRRNVFRLTTSDCECLFQAEDRDDMLSWIKTIQESSNLNEEDTGVTNRDLISRR
  IKEYNSLLSKTEQLPKTPRQSLSIRQTLLGAKSEPKTQSPHSPKEESERKLLSKDDTSPPKDKGTWRRGI
  PSIVRKTFEKKPAATGTFGVRLDDCPPAHTNRYIPLIVDICCKLVEERGLEYTGIYRVPGNNAAISSMQE
  ELNKGMADIDIQDDKWRDLNVISSLLKSFFRKLPEPLFTNDKYADFIEANRKEDPLDRLRTLKRLIHDLP
  EHHFETLKFLSAHLKTVAENSEKNKMEPRNLAIVFGPTLVRTSEDNMTHMVTHMPDQYKIVETLIQHHDW
  FFTEEGAEEPLTAVQEENTVDSQPVPNIDHLLTNIGRTGVLPGDVSDSATSDSAKSKGSWGSGKDQYSRE
  LLVSSIFAAASRKRKKPKEKAQPSSSEDELDSVFFKKENTEQSHSEIKEESKRESETSGSKQRVVVAKES
  NTKKDSGTTKEEKKIPWEEPPPPHSSKRNRSPTLSCRLAMLKEGPRSLLTQKPHCEETGSDSGTLLSTSS
  QASLLRSSTKKSTSPETKHSEFLSIAGTTTSDYSTTSSTTYLTSLDSSRLSPEVQSVAESKGDEADDERS
  ELVSEGRPVETDSESEFPVFPTTLTSDRLFRGKFQEVARVSRRNSEGSEASCTEGSLTPSLDSRRQQFSS
  HRLIECDTLSRKKSARFKSDSGSPGDTRTEKETPALAKMFDVMKKGKSTGSLLTPSRSESEKQEATWKTK
  IADRLKLRPRAPADDMFGVGNQKPTAETAKRKNIKRRHTLGGHRDATEISVLSFWKAHEQSADKESELSA
  VNRLKPKCSAQDLSISDWLARERVRTSASDLSRGEGLEPQAESPSVLGTPISTHSPPSQQPEARVAATST
  LASTSQSPLFTPPQSPDQINRESFQNMSQNASSTANIHPHKQSESPDTKAETPP
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC076629
• ORF Size: 5834 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC076629, AAH76629
• RefSeq Size: 6944 bp
• RefSeq ORF: 5834 bp
• Locus ID: 71435
• Cytogenetics: 2 A3
• Gene Summary: Functions as a GTPase-activating protein (GAP) for RHOA and CDC42. Downstream partner of ARF1 which may control Golgi apparatus structure and function. Also required for CTNNA1 recruitment to adherens junctions (By similarity).[UniProtKB/Swiss-Prot Function]