Plcb3 (NM_008874) Mouse Tagged ORF Clone

CAT#: MG211834

Plcb3 (tGFP-tagged) - Mouse phospholipase C, beta 3 (Plcb3)

Product Images

Circular map for MG211834

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Plcb3
• Synonyms: mKIAA4098
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG211834 representing NM_008874
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGGCGCGAGGCCCGGCGTCCACGCGCTGCAGCTGGAGCCGCCCACCGTGGTGGAGACCCTGCGGC
  GCGGGAGTAAGTTCATCAAATGGGACGAGGAGGCCTCCAGTCGGAACCTGGTGACCCTGCGTGTGGACCC
  TAATGGCTTCTTCTTGTACTGGACAGGACCCAACATGGAGGTGGACACACTGGACATCAGCTCCATCAGG
  GACACAAGGACAGGTCGTTATGCCCGCCTGCCCAAGGACCCTAAGATTCGAGAAGTACTGGGCTTTGGAG
  GTCCTGACACCCGGCTGGAGGAGAAACTGATGACAGTGGTGGCCGGGCCAGATCCAGTAAATACCACATT
  CTTGAACTTCATGGCCGTGCAAGATGACACAGTCAAGGTCTGGTCAGAGGAGTTGTTTAAACTGGCCATG
  AACATATTGGCTCAGAACGCCTCCCGGAACACCTTCCTGCGGAAAGCATACACGAAGCTGAAGCTGCAGG
  TGAACCAGGATGGACGGATTCCGGTCAAGAACATTCTGAAGATGTTCTCGGCGGACAAGAAGCGGGTGGA
  GACGGCGCTGGAGTCCTGTGGCCTCAACTTCAACCGAGTTGAGTCCATTCGCCCTGATGAGTTTCCCTTG
  GAAATTTTTGAGCGGTTCTTGAATAAACTGTGTCTGCGCCCAGATATTGACAAGATCCTGCTGGAGATAG
  GTGCCAAGGGCAAGCCGTACCTCACTCTGGAGCAGCTCATGGACTTTATCAACCAGAAGCAGAGAGACCC
  GAGACTCAACGAAGTGCTGTACCCGCCACTTCGGTCCTCACAGGCTCGGCTGCTCATTGAGAAGTACGAG
  ACGAACAAACAGTTCCTGGAGCGCGACCAGATGTCTATGGAGGGCTTCAGCCGCTACCTGGGAGGGGAGG
  AGAATGGTATCCTGCCTCTGGAGGCCCTGGATCTGAGCATGGACATGACCCAGCCACTGAGCGCATATTT
  TATCAACTCCTCACACAACACCTATCTCACTGCGGGCCAGCTGGCTGGACCATCATCGGTGGAGATGTAC
  CGCCAGGCACTGCTGTGGGGCTGCCGCTGTGTGGAGCTGGATGTATGGAAGGGACGGCCACCGGAGGAAG
  AGCCTTTCATCACTCATGGTTTCACCATGACCACTGAGGTGCCATTGCGTGATGTGCTAGAGGCCATCGC
  TGAGGCAGCCTTCAAGACCTCGCCCTACCCTGTCATCCTCTCCTTTGAGAACCATGTCGACTCGGCAAAG
  CAGCAGGCCAAGATGGCTGAGTACTGCCGCTCTATCTTTGGGGATGCGCTGCTCATTGACCCTCTCGACA
  AATACCCGCTATCTGCGGGCATCCCCCTGCCTAGTCCACAGGACCTGATGGGCCGTATCCTGGTGAAGAA
  TAAGAAGCGACATCGGCCCAGCACAGGTGTCCCTGACAGCTCAGTGCGCAAGCGGCCTCTGGAACAGAGC
  AACTCGGCCTTGAGTGAGAGCTCGGCCGCCACAGAGCCCTCCTCACCTCAGCTTGGGTCCCCCAGCTCCG
  ACAGCTGCCCTGGCCTAAGCAATGGGGAGGAGGTGGGACTCGAGAAGACCAGCCTGGAGCCTCAGAAGTC
  TCTAGGTGAGGAGAGCCTCAGCCGGGAACCTAATGTGCCCATGCCTGACCGTGACCGAGAGGATGAGGAG
  GAAGATGAAGAAGAAGAGGAAACAACGGATCCAAAAAAGCCCACCACTGATGAGGGCACAGCCAGCAGTG
  AGGTCAATGCCACGGAGGAGATGTCAACGCTCGTCAACTACGTTGAGCCCGTCAAGTTCAAGTCCTTCGA
  GGCTGCTCGAAAAAGGAACAAGTGCTTCGAGATGTCATCCTTCGTGGAGACCAAGGCAATGGAGCAACTG
  ACCAAGAGTCCTATGGAGTTTGTGGAATACAACAAACAGCAGCTCAGCCGCATCTACCCTAAGGGCACAC
  GAGTGGACTCCTCCAACTACATGCCTCAGCTCTTCTGGAACGTGGGTTGCCAGCTTGTTGCCCTCAACTT
  CCAGACCTTGGATTTGCCAATGCAGCTCAACGCAGGCGTGTTTGAGTACAATGGGCGCAGCGGGTACTTG
  CTCAAGCCCGAGTTTATGCGGCGGCCGGACAAGTCTTTTGATCCCTTCACTGAGGTCATCGTAGATGGCA
  TAGTGGCCAACGCCTTGCGGGTCAAGGTGATTTCGGGGCAGTTCCTGTCTGACAAGAAGGTGGGCATCTA
  CGTCGAGGTGGACATGTTTGGCCTCCCAGTTGACACAAGACGCAAATATCGTACCCGGACATCCCAGGGG
  AACTCATTCAACCCTGTGTGGGACGAGGAACCCTTTGACTTCCCCAAGGTTGTGCTGCCCACGCTGGCCT
  CACTTCGCATCGCAGCCTTTGAGGAGGGTGGCAAATTTGTTGGGCACCGTATCCTGCCTGTCTCTGCTAT
  CCGCTCAGGATACCACTATGTCTGCCTGCGAAACGAGGCCAACCAACCTTTGTGCCTGCCTGCCCTGCTT
  ATCTACACTGAAGCTTCTGACTACATCCCAGATGACCACCAGGACTATGCGGAGGCCTTGATTAACCCCA
  TCAAGCACGTAAGCCTAATGGACCAGCGGGCCAAGCAACTAGCCGCTCTCATTGGGGAGAGTGAGGCTCA
  GGCCAGCACAGAGACATATCAGGAGACCCCGTGTCAACAGCCAGGGTCACAGCTCCCCTCCAACCCCACA
  CCTAACCCACTGGATGCCTCACCTCGCTGGCCCCCCGGTCCTACCACTTCCTCCACTAGCTCCTCCCTCA
  GTAGCCCAGGGCAGCGAGATGATTTGATTGCCAGCATCCTCTCTGAGGTGACCCCTACACCTCTAGAGGA
  GCTCAGAAGCCACAAGGCTATGGTGAAGCTACGGAGCCGGCAGGATCGAGACCTGCGGGAGTTGCATAAG
  AAGCACCAGCGCAAGGCTGTGGCCCTCACCCGCCGCCTTCTGGATGGCCTGGCCCAGGCCAGGGCTGAGG
  GGAAGTGCCGGCCCTCCCCCAGTGCCCTGGGCAAGGCCACCAACTCGGAGGACGTGAAGGAGGAAGAGGA
  GGCCAAACAGTATCGAGAGTTCCAGAACCGACAAGTACAGAGCCTGTTGGAGCTGAGGGAAGCCCAGGCA
  GATGTGGAGACCAAGCGGAAGCTGGAGCATCTACGACAGGCTCATCAGCGGCTCAAGGAGGTTGTCCTGG
  ATACACACACAACACAGTTCAAGAGGCTGAAGGAGCTGAATGAAAGGGAGAAGAAGGAACTCCAGAAGAT
  CCTGGACAGGAAGCGCAACAACAGCATCTCAGAGGCCAAGACAAGGGAGAAACACAAGAAGGAGGTGGAA
  CTGACAGAGATTAATCGGCGGCACATCACTGAGTCGGTAAACTCCATCAGACGGCTGGAAGAGGCCCAGA
  AGCAGCGCCATGAACGCCTGGTGGCAGGGCAACAGCAAGTCCTCCAGCAGCTAGAGGAAGAGGAACCCAA
  GCTGCTGGCCCAGCTGACCCAGGAGTGTCAGGAACAGCGAGAGAGGTTGCCCCAGGAGATCCGTCGGTGC
  CTGCTGGGCGAGACAGCAGAGGGACTGGGGGACGGCCCCCTGGTGGCCTGTGCCAGCAATGGTCATGCAC
  CTGGGAGTGGTGGGCACCTGTCCAGCGCTGACTCGGAGAGCCAGGAGGAGAACACCCAGCTT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG211834 representing NM_008874
  Red=Cloning site Green=Tags(s)
  
  MAGARPGVHALQLEPPTVVETLRRGSKFIKWDEEASSRNLVTLRVDPNGFFLYWTGPNMEVDTLDISSIR
  DTRTGRYARLPKDPKIREVLGFGGPDTRLEEKLMTVVAGPDPVNTTFLNFMAVQDDTVKVWSEELFKLAM
  NILAQNASRNTFLRKAYTKLKLQVNQDGRIPVKNILKMFSADKKRVETALESCGLNFNRVESIRPDEFPL
  EIFERFLNKLCLRPDIDKILLEIGAKGKPYLTLEQLMDFINQKQRDPRLNEVLYPPLRSSQARLLIEKYE
  TNKQFLERDQMSMEGFSRYLGGEENGILPLEALDLSMDMTQPLSAYFINSSHNTYLTAGQLAGPSSVEMY
  RQALLWGCRCVELDVWKGRPPEEEPFITHGFTMTTEVPLRDVLEAIAEAAFKTSPYPVILSFENHVDSAK
  QQAKMAEYCRSIFGDALLIDPLDKYPLSAGIPLPSPQDLMGRILVKNKKRHRPSTGVPDSSVRKRPLEQS
  NSALSESSAATEPSSPQLGSPSSDSCPGLSNGEEVGLEKTSLEPQKSLGEESLSREPNVPMPDRDREDEE
  EDEEEEETTDPKKPTTDEGTASSEVNATEEMSTLVNYVEPVKFKSFEAARKRNKCFEMSSFVETKAMEQL
  TKSPMEFVEYNKQQLSRIYPKGTRVDSSNYMPQLFWNVGCQLVALNFQTLDLPMQLNAGVFEYNGRSGYL
  LKPEFMRRPDKSFDPFTEVIVDGIVANALRVKVISGQFLSDKKVGIYVEVDMFGLPVDTRRKYRTRTSQG
  NSFNPVWDEEPFDFPKVVLPTLASLRIAAFEEGGKFVGHRILPVSAIRSGYHYVCLRNEANQPLCLPALL
  IYTEASDYIPDDHQDYAEALINPIKHVSLMDQRAKQLAALIGESEAQASTETYQETPCQQPGSQLPSNPT
  PNPLDASPRWPPGPTTSSTSSSLSSPGQRDDLIASILSEVTPTPLEELRSHKAMVKLRSRQDRDLRELHK
  KHQRKAVALTRRLLDGLAQARAEGKCRPSPSALGKATNSEDVKEEEEAKQYREFQNRQVQSLLELREAQA
  DVETKRKLEHLRQAHQRLKEVVLDTHTTQFKRLKELNEREKKELQKILDRKRNNSISEAKTREKHKKEVE
  LTEINRRHITESVNSIRRLEEAQKQRHERLVAGQQQVLQQLEEEEPKLLAQLTQECQEQRERLPQEIRRC
  LLGETAEGLGDGPLVACASNGHAPGSGGHLSSADSESQEENTQL
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_008874
• ORF Size: 3702 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_008874.2
• RefSeq Size: 4195 bp
• RefSeq ORF: 3705 bp
• Locus ID: 18797
• UniProt ID: P51432
• Cytogenetics: 19 5.1 cM
• Gene Summary: The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes.[UniProtKB/Swiss-Prot Function]