Dopey2 (BC057081) Mouse Tagged ORF Clone

CAT#: MG211830

Dopey2 (tGFP-tagged) - Mouse dopey family member 2 (cDNA clone MGC:67481 IMAGE:5704600)

Product Images

Circular map for MG211830

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Dopey2
• Synonyms: mKIAA0933, C21orf5
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG211830 representing BC057081
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGACAGTCCCTCCCATTGCTCCTGGGATACTGAAAGTCATTGCCGAGAAAACAGACTTCTATCAGA
  GGGTGGCTCGTGTGCTCTGGAACCAGCTGAACAAGGAGACCCGTGAACATCACATCACCTGTGTAGAGCT
  GTTCTACCGCCTGCACTGCCTGGCCCCGACTGCCAACATCTGTGAGGATATCATCTGCCATGCCCTCCTG
  GACCCTGACAAGGGAACAAGGTTGGAAGCTCTGTTTAGGTTTTCTGTGATCTGGCACCTGACACGAGAAA
  TCCAAGGTAGTCGTGTGACGTCCCACAATCGCTCCTTTGACAGGTCCTTGTTTGTCGTCCTGGATAGCCT
  GGCTTGCACAGACGGTGCTATCAGTGCAGCAGCCCAGGGCTGGCTGGTGCGAGCGCTCTCCCTCGGGGAT
  GTGGCCCGCATCCTCGAACCCATGCTCCTGCTGTTGCTCCAGCCCAAAACCCAGAGGACGTCCATCCAGT
  GTCTCAAGCAGGAGAACTCAGCCGAGGATTTACACCGTTGGTTTAACAGGAAGAAGCCCACGTGCAAAGA
  GGCGTGTGGTGAATCTGAGCCGCAGGAGGGTGCCCCTGAGGAACGCCTGCCTCGAGGACAGTTCACCACC
  GTGGATCGAGAAGCCATCTGGGCTGAAGTAGAGAAGGAACCAGAGAAATGCCCACCAAGAAGTGATCTAA
  GTGAGGAAGACCTGCCCTACTATGTGGACCTTCCAGACAGGATGACCTCTGGTGGCGGGGATAGCAGTGA
  GCACACAGAGTCTGCAGACACGAGCTCTGGCCACACTGACAGTGAGAACACATCCACTTTCTCATCTCCC
  TCCCACGATCTGCAGGACCTGAGCCACGAGGAAAACTGCTGTGCTCCGATCCCCATCGGGGGCAGGGCAT
  ACTCCAAGCGAGCGGCCTTGCTGGCAGCCTTCCAGCCAGAAAGCCCCAGGTCCAATGCCAGGTTAAGCCT
  GGTGCGAGCAGACTCAGACAAGACACAGGCTTCCGAGTCGTTTTCCAGTGACGAGGAGGCTGACGTGGAG
  CTCCAAGCCATCACCACATCGAGGCTGTTGAAGCAGCAGAGGGAGAAGCAGGAGACAGTCGAGGCCTTGT
  TCAAACACATCTTGCTCTACCTGCAGCCCTACGACTCCCAGAGGGTCCTGTATGCCTTCTCCGTACTGGA
  GGCCGTGCTTAAGACCAATCCCAAGGAGTTCATTGAGGCTGTGTCCAGGACGGGCATAGACACCAGCTCC
  ACCGCGCACCTCAACCTCATCTCCAACCTCCTGGCACGCCACCAGGAGGCCCTCATCGGCCAGAGCTTTT
  ATGGCAAGCTGCAGACCCAGGCCCCCAATGTCTGCCCCCACTCCCTGCTCATTGAACTCCTTACCTACCT
  CTGCCTGAGCTTCCTCCGCTCCTACTACCCCTGCTACCTGAAGGTCTGCCACAGGGACATCCTTGGCAAC
  CGCGACGTGCAGGTCAAGAGCGTGGAGGTCCTCATTAGGATTACAGCACAGCTGGTCTCCATGGCCAAGT
  CGGCGGAAGGGAAGAACACGGAGTTCATCCACAGCCTGCTGCAGAGGTGCAAGGTCCAGGAGTTCGTCCT
  GCTCTCGCTGTCCGCGTCCATGTACACCAGCCAGAAGCGCTACGGCCTGGCCACCGCCGACCGGGGCGGC
  AGGCTCCTCGCGGAGGACAGCCTCTTTGAGGAGAGCCTCATCAATCTGGGGCAGGACCAGATCTGGAGCG
  AGCACCCACTGCAGATCGAGCTGCTCAAGCTGCTGCAGGCCCTCATCGTCCTGGAGCACCACCTGGGCCA
  GGGCCAGGAGGAGGCGGAGACTCAGCCGGCCCTATCCCGAGAGTGGCAGAGGGCTCTCAACTTCCAGCAG
  GCCATCGGTGCCATGCAGTACGTGCAGCCCCACCCCCTCACCTCCCAGGGCCTTCTGGTCTCAGCTGTGG
  TGAGAGGGCTGCAGCCCGCCTATGGCTACGGCATGCACCCCGCCTGGGTGAGCCTGGTCACGCATTCCTT
  GCCATACTTTGGAAAGTCTCTGGGCTGGACAGTGACACCCTTTGTTATCCAGATTTGCAAAAATTTGGAC
  GATCTGGTCAAGCAATATGAAAGTGAATCTGTCAAGTTCACCATCAGTACAACCTCAAAGAAGGAAAACA
  TTTCTCCTGACTACCCGCTCACTCTTCTGGAAGGTCTTACGACCATTAGCCATTTTTGTCTTTTGGAACA
  GCCTACCCAACATAAAAAGACTGCTGCCGTAAGCGATCCCATCAACCTGAGAAATGCCAAGAACGCCATT
  TTGGAAGAGCTACCTCGAATAGTGAACACCATGGCCCTTATCTGGAATGTTCTCAGAAAGGAGGAAACTC
  AGAAGAGACCAGTGGATCTCCTGGGAGCCACCAAAGGGTCATCCTCTGTTTACTTTAAAACCACCAAGAC
  CATCAGACAAAAAATTTTAGACTTGCTGAACCCCTTGACAGGCCATCTTGGGGTCCAGCTGATAGCTGCG
  GTTGCCACCGTGTGGAACAGAAAGCAGGCACGGCGCCACAGCAAGACCAAGATGGTCCCCGTGGCAAACA
  CGTCCCAGCACACCCTTGTCGACCTGGTCTGTGCTCTCAGCACCCTGCAGACAGACTCAGTGCTGCAGCT
  GGTGAAGGAGGTGGTGAAGAGGCCAGCGCAGATCAAAGGGGATGAGAAGTCTCCCCTGGTGGACATTCCT
  GTGTTGCAGTTCTGCTATGCTTTCATCCAAAGACTGTCCATACCAGACCTGCAGGAGGCCTTCCCTTCCC
  TCCTGGGCGTCCTGAAGGAGGCTGCGCAGCTGAACCTGGCTCCCCCTGGATACTTTCTGCTTCTCAGCAT
  GTTGAATGACTTTGTGACAAGAACACCCAACCTGGAAAGCAAGAAGGACCAAAAAGACCTGCAGGAAATC
  ACTCAGAGAATCCTGGAAGCTGTAGGTACCATCGCTGGCTCCTCCTTGGAACAAACAAGCTGGCTCAGCA
  GAAATCTGGAGGTGAAAGCCCAACCTCAGGTCTCTCTGGAAGAGTCAGATGCCGAGGACGACGTGCACAG
  TGCTGCTGAAGCCTCCACCATGGTATCTGCTTCTGCCCCCTCCGTTTACAGTGTGCAAGCGCTCTCTCTC
  TTGGCAGAGGTTCTGGCTTCTCTCCTGGACATGGTTTACCGCAGCGACGAGAAAGAGAAAGCTGTACCAC
  TAATCTCCCGCTTGCTATACTACGTGTTTCCTTACCTGCGCAATCACAGTGCGTACAATGCTCCCAGCTT
  CCGAGCCGGCGCCCAGCTGCTGAGCTCCCTGAGTGGCTATGCCTACACCAAGCGGGCGTGGAAGAAGGAA
  GTGCTGGAGCTGTTCTTGGACCCCGCTTTCTTTCAGATGGATACGTCTTGCGTCCACTGGAAGTCCGTCA
  TTGACCATCTCCTGACTCACGAGAAAACCATGTTTAAGGATTTAATGAACATGCAGAGCAGTTCCTTAAA
  ACTCTTCTCCAGCTTTGAGCAGAAGGCCATGCTGCTGAAGCGCCAGGCTTTTGCCGTCTTCAGTGGCGAG
  CTTGACCAGTACCACCTCTACCTTCCCCTGATACAAGATGTTTCTTTTTTTCAGAGTTCTGCTGCTAAGA
  ATCTCTCCCCAGCACCTGACCTCGCTGTGGCCCATCATGGTGTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG211830 representing BC057081
  Red=Cloning site Green=Tags(s)
  
  MVTVPPIAPGILKVIAEKTDFYQRVARVLWNQLNKETREHHITCVELFYRLHCLAPTANICEDIICHALL
  DPDKGTRLEALFRFSVIWHLTREIQGSRVTSHNRSFDRSLFVVLDSLACTDGAISAAAQGWLVRALSLGD
  VARILEPMLLLLLQPKTQRTSIQCLKQENSAEDLHRWFNRKKPTCKEACGESEPQEGAPEERLPRGQFTT
  VDREAIWAEVEKEPEKCPPRSDLSEEDLPYYVDLPDRMTSGGGDSSEHTESADTSSGHTDSENTSTFSSP
  SHDLQDLSHEENCCAPIPIGGRAYSKRAALLAAFQPESPRSNARLSLVRADSDKTQASESFSSDEEADVE
  LQAITTSRLLKQQREKQETVEALFKHILLYLQPYDSQRVLYAFSVLEAVLKTNPKEFIEAVSRTGIDTSS
  TAHLNLISNLLARHQEALIGQSFYGKLQTQAPNVCPHSLLIELLTYLCLSFLRSYYPCYLKVCHRDILGN
  RDVQVKSVEVLIRITAQLVSMAKSAEGKNTEFIHSLLQRCKVQEFVLLSLSASMYTSQKRYGLATADRGG
  RLLAEDSLFEESLINLGQDQIWSEHPLQIELLKLLQALIVLEHHLGQGQEEAETQPALSREWQRALNFQQ
  AIGAMQYVQPHPLTSQGLLVSAVVRGLQPAYGYGMHPAWVSLVTHSLPYFGKSLGWTVTPFVIQICKNLD
  DLVKQYESESVKFTISTTSKKENISPDYPLTLLEGLTTISHFCLLEQPTQHKKTAAVSDPINLRNAKNAI
  LEELPRIVNTMALIWNVLRKEETQKRPVDLLGATKGSSSVYFKTTKTIRQKILDLLNPLTGHLGVQLIAA
  VATVWNRKQARRHSKTKMVPVANTSQHTLVDLVCALSTLQTDSVLQLVKEVVKRPAQIKGDEKSPLVDIP
  VLQFCYAFIQRLSIPDLQEAFPSLLGVLKEAAQLNLAPPGYFLLLSMLNDFVTRTPNLESKKDQKDLQEI
  TQRILEAVGTIAGSSLEQTSWLSRNLEVKAQPQVSLEESDAEDDVHSAAEASTMVSASAPSVYSVQALSL
  LAEVLASLLDMVYRSDEKEKAVPLISRLLYYVFPYLRNHSAYNAPSFRAGAQLLSSLSGYAYTKRAWKKE
  VLELFLDPAFFQMDTSCVHWKSVIDHLLTHEKTMFKDLMNMQSSSLKLFSSFEQKAMLLKRQAFAVFSGE
  LDQYHLYLPLIQDVSFFQSSAAKNLSPAPDLAVAHHGV
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC057081
• ORF Size: 3684 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC057081.1
• RefSeq Size: 4743 bp
• RefSeq ORF: 3686 bp
• Locus ID: 70028
• Cytogenetics: 16 C4
• Gene Summary: May be involved in protein traffic between late Golgi and early endosomes.[UniProtKB/Swiss-Prot Function]