Ift122 (NM_031177) Mouse Tagged ORF Clone

CAT#: MG211768

Ift122 (tGFP-tagged) - Mouse intraflagellar transport 122 homolog (Chlamydomonas) (Ift122)

Product Images

Circular map for MG211768

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Ift122
• Synonyms: C86139; sopb; Wdr10
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG211768 representing NM_031177
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGGGCCGTGTTGACGTGGAGAGACAAAGCGGAGCAGTGTATCTACGACCTCGCATTTAAGCCTGATG
  GAACACAACTGATTCTGGCTGCAGGAAACAGATTGCTGGTTTATGACACCTCTGATGGCACCTTACTTCA
  GCCCCTGAAGGGACACAAAGACACCGTGTACTGTGTGGCGTATGCAAAGGATGGCAAGCGCTTTGCATCT
  GGATCTGCTGACAAGAGCATCATTATCTGGACCTCAAAACTTGAAGGCATTCTGAAGTACACGCACAACG
  ACTCTATTCAGTGTGTCTCCTACAATCCTGTTACCCACCAGCTGGCATCTTGTTCCTCCAGTGACTTTGG
  GTTATGGTCTCCTGAGCAGAAGTCTGTGTCCAAGCACAAATCCAGCAGCAAGATCACGTGCTGCAGCTGG
  ACAAACGATGGTCAGTACCTGGCTCTGGGCATGGCCAATGGCATCATCAGTATACGGAACAAGAATGGTG
  AAGAGAAAGTGAAGATCGAGCGCCCTGGGGGCTCCCTCTCCCCAATATGGTCCATCTGCTGGAACCCTTC
  AAGAGAGGAACATAATGACATCCTGGCTGTAGCTGACTGGGGACAGAAACTTTCCTTCTACCAGCTGAGT
  GGAAAACAGATTGGGAAGGATCGGCCACTGAACTTTGACCCCTGTTGCATCAGCTACTTCACTAAAGGAG
  AGTACATTTTGGTGGGAGGTTCAGACAAGCAGGTATCTCTTTTCACCAAGGATGGAGTGCGACTGGGGAC
  CGTTGGGGAGCAGAACTCCTGGGTGTGGACCTGTAGAGTGAAACCTGACTCCAATTATGTGGTGGTCGGC
  TGCCAGGATGGCACCATCTCCTTCTACCAGCTCATCTTCAGCACAGTCCATGGGCTCTACAAAGATCGGT
  ATGCCTACAGGGACAGCATGACAGACGTCATCGTGCAGCACCTCATCACAGAGCAGAAAGTTAGGATTAA
  GTGCAGAGAGCTTGTCAAGAAAATTGCCATCTATAAAAATCGATTAGCTATCCAGCTGCCAGAGAAGATC
  CTCATCTACGAGCTGTACTCTGAAGACTCGACAGACATGCACTACCGGGTAAAGGAGAAAATTGTCAAGA
  AGTTTGAGTGCAACCTGCTGGTGGTGTGTGCTGACCACATCATCCTGTGCCAGGAGAAACGGCTACAGTG
  CCTGTCCTTCAGTGGAGTGAAGGAAAGGGAATGGCAGATGGAATCTCTCATCCGCTACATCAAGGTGATC
  GGTGGCCCTGCTGGAAGAGAAGGTCTGTTGGTAGGCCTGAAAAACGGCCAGATCCTGAAGATCTTTGTGG
  ACAATCTTTTTGCTATTGTCCTGCTGAAGCAGGCCACAGCCGTGCGCTGCCTGGACATGAGTGCCTCCCG
  GAACAAGCTGGCTGTGGTGGATGAGAATGACACGTGCCTGGTGTACGACATCCACACGAAGGAACTGCTT
  TTCCAGGAACCGAATGCTAACAGCGTGGCCTGGAACACGCAGTGTGAGGACATGCTCTGCTTCTCTGGAG
  GAGGCTACCTCAACATCAAGGCCAGCACCTTCCCAGTGCACCAGCAGAAGCTGCAGGGCTTTGTGGTTGG
  CTACAACGGCTCCAAGATCTTCTGCCTTCATGTCTTCTCTATGTCTGCCGTGGAGGTGCCACAGTCCGCT
  CCAATGTACCAGTACCTCGATAGGAAGATGTTCAAAGAAGCATATCAGATCGCTTGCTTGGGAGTGACAG
  ACGCTGATTGGCGTGAACTGGCCATGGAAGCTCTAGAAGGACTAGAGTTTGAGACGGCAAGGAAGGCCTT
  CACCAGAGTCCAGGACCTTCGATACCTAGAACTGATCAGCAGTATTGAGGAGAGGAAGAAGAGGGGAGAG
  ACCAACAATGACCTGTTTCTGGCAGATGTGTTTTCCTACCAGGGGAAGTTTCATGAAGCCGCCAAACTAT
  ACAAGAGGAGCGGGCATGAGAACCTCGCTCTCGACATGTATACTGACCTCTGCATGTTTGAGTACGCCAA
  GGATTTCCTTGGATCTGGAGACCCTAAAGAAACGAAGATGCTCATCACCAAGCAGGCCGACTGGGCCAGA
  AACATCAATGAGCCCAAAGCTGCAGTGGAGATGTACATCTCAGCAGGGGAGCACGCCAAGGCCATAGAGA
  TCAGCGGCAGCCATGGCTGGGTGGACATGTTGATAGACATTGCTCGGAAGCTGGACAAGGCTGAACGGGA
  GCCCCTGCTCATGTGTGCTTGCTACTTTAAGAAGCTGGACAGTCCTGGCTATGCTGCTGAGACCTACCTG
  AAGATCGGGGACCTGAAGTCTCTGGTGCAGCTGTATGTGGACACCAAGCGCTGGGATGAGGCCTTTGCGT
  TAGGTGAGAAGCACCCGGAGTTCAAGGATGACGTCTACGTGCCCTACGCGCAGTGGCTGGCGGAGAACGA
  CCGCTTTGAGGAAGCCCAGAAAGCCTTCCACAAGGCCGGGAGGCAGGGAGAAGCAGTCCGGGTGCTGGAG
  CAGCTCACGCACAATGCTGTGGTGGAGAGCAGGTTTAACGACGCTGCCTATTATTACTGGATGCTGTCCA
  TGCAGTGCCTCGACATGGCTCAAGATCCTGCCCAGAAGGACGCGATGCTGGACAAGTTTCACCACTTCCA
  ACACCTGGCTGAGCTGTACCATGGCTATCAAACCATTCACCGATACACGGAGGAGCCATTCAGCTTTGAC
  CTCCCTGAAACCCTCTTCAACATCTCCAAGTTCTTGCTGCACAGCCTGACCAAGGCCACCCCCTTGGGTA
  TCTCCAAAGTGAATACACTCTTCACACTGGCTAAGCAGAGCAAGGCTCTGGGTGCTTACAAGCTGGCCCG
  GCATGCCTATGACAAGCTACGGGGGCTGCAGATCCCCGCCAGGATCCAGAAGTCCATTGAGCTGGGCACC
  CTGACCATCCGCTCCAAGCCCTTCCATGACAGTGAGGAGCTGGTACCCTTGTGCTACCGCTGTTCTACCA
  ACAACCCGCTGCTGAACAACCTTGGCAACGTCTGCATCAACTGCCGCCAGCCCTTCATCTTCTCCGCCTC
  TTCCTATGAGGTGCTGCACCTGGTGGAATTCTACCTGGAAGAGGGGATCACTGACGAAGAAGCTGTTGCC
  CTCATCGATCTGGAGGCACCAAGACACAAGAGGGAGGGCAAGTGGCGAGAGACCTCAAGCAACAATTCCC
  AGACGTTGAAGCTGGACGAGACCATGGACTCCATAGGAGAAGATGACCCATTTACAGCCAAGCTGAGCTT
  TGAGCAAGGTAGCTCTGAGTTTGTGCCTGTGGTGGTGAACCGGTCAGTACTACGCTCCATGAGCCGAAGA
  GATGTTCTCATCAAGCGCTGGCCCCCGCCGCTGCAATGGCAATATTTCCGCTCGCTGTTGCCTGATGCCT
  CCATCACCATGTGCCCCTCCTGCTTTCAGATGTTCCATTCAGAGGACTACGAACTGCTTGTGCTCCAGCA
  CGCCTGCTGCCCTTACTGCCGAAGGCGTATCGACGACACTGGCCCG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG211768 representing NM_031177
  Red=Cloning site Green=Tags(s)
  
  MRAVLTWRDKAEQCIYDLAFKPDGTQLILAAGNRLLVYDTSDGTLLQPLKGHKDTVYCVAYAKDGKRFAS
  GSADKSIIIWTSKLEGILKYTHNDSIQCVSYNPVTHQLASCSSSDFGLWSPEQKSVSKHKSSSKITCCSW
  TNDGQYLALGMANGIISIRNKNGEEKVKIERPGGSLSPIWSICWNPSREEHNDILAVADWGQKLSFYQLS
  GKQIGKDRPLNFDPCCISYFTKGEYILVGGSDKQVSLFTKDGVRLGTVGEQNSWVWTCRVKPDSNYVVVG
  CQDGTISFYQLIFSTVHGLYKDRYAYRDSMTDVIVQHLITEQKVRIKCRELVKKIAIYKNRLAIQLPEKI
  LIYELYSEDSTDMHYRVKEKIVKKFECNLLVVCADHIILCQEKRLQCLSFSGVKEREWQMESLIRYIKVI
  GGPAGREGLLVGLKNGQILKIFVDNLFAIVLLKQATAVRCLDMSASRNKLAVVDENDTCLVYDIHTKELL
  FQEPNANSVAWNTQCEDMLCFSGGGYLNIKASTFPVHQQKLQGFVVGYNGSKIFCLHVFSMSAVEVPQSA
  PMYQYLDRKMFKEAYQIACLGVTDADWRELAMEALEGLEFETARKAFTRVQDLRYLELISSIEERKKRGE
  TNNDLFLADVFSYQGKFHEAAKLYKRSGHENLALDMYTDLCMFEYAKDFLGSGDPKETKMLITKQADWAR
  NINEPKAAVEMYISAGEHAKAIEISGSHGWVDMLIDIARKLDKAEREPLLMCACYFKKLDSPGYAAETYL
  KIGDLKSLVQLYVDTKRWDEAFALGEKHPEFKDDVYVPYAQWLAENDRFEEAQKAFHKAGRQGEAVRVLE
  QLTHNAVVESRFNDAAYYYWMLSMQCLDMAQDPAQKDAMLDKFHHFQHLAELYHGYQTIHRYTEEPFSFD
  LPETLFNISKFLLHSLTKATPLGISKVNTLFTLAKQSKALGAYKLARHAYDKLRGLQIPARIQKSIELGT
  LTIRSKPFHDSEELVPLCYRCSTNNPLLNNLGNVCINCRQPFIFSASSYEVLHLVEFYLEEGITDEEAVA
  LIDLEAPRHKREGKWRETSSNNSQTLKLDETMDSIGEDDPFTAKLSFEQGSSEFVPVVVNRSVLRSMSRR
  DVLIKRWPPPLQWQYFRSLLPDASITMCPSCFQMFHSEDYELLVLQHACCPYCRRRIDDTGP
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_031177
• ORF Size: 4080 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_031177.1
• RefSeq Size: 4059 bp
• RefSeq ORF: 3552 bp
• Locus ID: 81896
• UniProt ID: Q6NWV3
• Cytogenetics: 6 E3
• Gene Summary: As a component of the IFT complex A (IFT-A), a complex required for retrograde ciliary transport and entry into cilia of G protein-coupled receptors (GPCRs), it is required in ciliogenesis and ciliary protein trafficking (By similarity). Involved in cilia formation during neuronal patterning. Acts as a negative regulator of Shh signaling. Required to recruit TULP3 to primary cilia (PubMed:19000668, PubMed:21209331).[UniProtKB/Swiss-Prot Function]