Slc12a6 (BC062099) Mouse Tagged ORF Clone

CAT#: MG211642

Slc12a6 (tGFP-tagged) - Mouse solute carrier family 12, member 6 (cDNA clone MGC:69652 IMAGE:6844732)

Product Images

Circular map for MG211642

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Slc12a6
• Synonyms: 9530023I19Rik; gaxp; KCC3
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG211642 representing BC062099
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCATCCACCAGAAGCCACCACCAAGATGTCCTCAGTTCGGTTCATGGTGACACCAACTAAGATTGATG
  ACATTCCAGGTTTGTCAGACACCAGCCCGGACCTCAGCTCTCGGTCTAGTTCTCGAGTAAGATTTAGCTC
  CCGAGAAAGTGTGCCAGAAACAAGCCGTAGTGAGCCTATGAGCGAACTGTCTGGGGCTACTACTTCTCTG
  GCAACTGTTGCCCTAGATCCTTCCAGTGACCGGACTTCTAATCCCCAGGATGTTACGGAGGATGACGGCC
  ATAAAAAAGCCCGAAATGCTTATCTCAATAATTCCAACTATGAAGAAGGAGACGAATATTTTGATAAAAA
  TTTGGCACTCTTTGAGGAAGAAATGGACACCAGACCAAAGGTGTCTTCTCTCCTCAACCGCATGGCCAAC
  TATACAAATCTGACACAAGGAGCAAAGGAACATGAAGAGGCAGAGAACATCACTGAAGGGAAAAAGAAGC
  CTACCAAGAGCCCCCAAATGGGTACTTTCATGGGTGTCTACCTCCCATGTCTACAGAACATCTTTGGAGT
  GATCCTCTTCCTGCGTCTTACCTGGGTAGTGGGAACAGCTGGAATCCTTCAGGCCTTTGCAATTGTCCTC
  ATCTGCTGCTGCTGTACAATGTTAACTGCCATCTCCATGAGCGCCATCGCCACTAACGGAGTGGTGCCAG
  CTGGGGGCTCATACTTCATGATTTCCAGAGCCCTGGGCCCAGAGTTTGGCGGGGCTGTAGGCCTCTGCTT
  TTATCTTGGCACCACATTTGCAGCAGCCATGTATATTCTTGGTGCCATTGAAATCTTTCTGGTATACATT
  GTCCCCCGAGCTGCCATCTTTCGGAGTGACGATGCACTCAAGGAGTCAGCAGCTATGCTGAACAACATGC
  GCGTCTATGGTACAGCCTTCTTGGTCCTCATGGTCTTGGTGGTATTCATCGGCGTACGCTATGTGAATAA
  GTTTGCCTCACTCTTCCTGGCCTGTGTAATTGTGTCGATCTTGGCTATCTATGCTGGTGCCATCAAGTCT
  TCCTTTGCTCCACCACACTTCCCGGTCTGTATGCTGGGCAACCGTACCCTGTCATCAAGACACCTTGACA
  TTTGCTCTAAGACCAAGGAGGTTGACAACATGACAGTACCATCAAAGTTATGGGGATTCTTCTGCAACTC
  GAGTCAGTTCTTTAATGCCACCTGTGATGAGTACTTTGTTCACAATAACGTCATCTCAATCCAAGGCATT
  CCAGGGTTGGCTAGTGGTATCATTACTGAAAATCTTTGGAGTAATTATTTACCAAAGGGTGAGATAATTG
  AAAAGCCATCAGCCAAGTCATCTGATGTCTTAGGCAACTTAAACCATGAATATGTTCTTGCTGATATCAC
  CACCTCCTTCACTCTGCTGGTGGGGATCTTCTTTCCCTCGGTCACAGGTATCATGGCTGGGTCAAACAGA
  TCTGGAGATCTGAAAGATGCCCAGAAGTCTATTCCCATTGGGACCATCCTTGCCATCCTGACCACATCCT
  TTGTGTATTTAAGCAATGTTGTCCTTTTTGGTGCATGTATTGAAGGAGTCGTTCTCAGAGACAAATTTGG
  GGATGCTGTAAAAGGGAATTTGGTTGTAGGCACCTTATCCTGGCCATCCCCGTGGGTGATCGTTATTGGC
  TCCTTCTTTTCAACATGTGGGGCTGGGCTGCAGAGCCTCACAGGTGCGCCTCGGCTGCTGCAGGCTATCG
  CCAAGGATAACATCATACCTTTCCTTAGGGTTTTTGGTCACAGCAAAGCTAATGGGGAACCTACCTGGGC
  TTTACTTCTAACTGCTGCCATAGCAGAGCTGGGAATTCTCATCGCCTCCCTGGATCTCGTGGCCCCAATT
  CTTTCCATGTTTTTTCTCATGTGTTACCTCTTTGTGAACTTGGCTTGTGCCTTGCAAACATTGCTGCGAA
  CCCCCAACTGGAGGCCTCGATTCCGCTATTATCACTGGGCCCTCTCTTTCATGGGAATGAGTATCTGTCT
  AGCTCTGATGTTCATTTCTTCTTGGTATTATGCCATTGTAGCTATGGTAATAGCTGGCATGATCTACAAG
  TACATTGAATATCAAGGGGCTGAGAAAGAATGGGGGGATGGTATCCGTGGGCTGTCGCTCAGTGCAGCCC
  GCTTCGCTTTGCTCCGTCTAGAGGAAGGACCTCCTCACACTAAAAACTGGAGGCCTCAGCTGCTCGTCCT
  ACTGAAGCTGGATGAAGATTTACACGTCAAGCACCCTCGCCTCCTCACCTTTGCCTCCCAGCTCAAGGCA
  GGAAAGGGACTCACGATTGTGGGCTCTGTCATCGTGGGGAACTTCTTGGAGAACTATGGTGACGCGCTCG
  CGGCAGAGCAGACCATTAAGCACCTAATGGAGGCAGAAAAGGTAAAAGGATTCTGCCAATTGGTGGTGGC
  TGCCAAGCTGAAAGAGGGCATTTCACACCTCATCCAGTCCTGTGGCCTCGGAGGCATGAAACACAACACA
  GTGGTGATGGGCTGGCCCAATGGCTGGCGTCAGAGTGAAGATGCTCGCGCTTGGAAGACTTTCATTGGCA
  CAGTACGAGTGACAACTGCTGCCCATCTAGCCCTGCTGGTGGCTAAAAATGTCTCCTTCTTTCCCAGCAA
  TGTGGAGCAGTTTTCTGAGGGCAACATTGATGTGTGGTGGATTGTGCATGATGGGGGCATGCTCATGCTA
  TTACCGTTCCTGCTGAAACAGCACAAGGTTTGGCGGAAATGCAGCATACGGATCTTCACAGTAGCCCAAC
  TAGAAGACAACAGTATCCAGATGAAGAAGGATCTGGCCACCTTTCTGTACCACCTGCGCATTGAGGCAGA
  AGTGGAAGTGGTGGAGATGCACGACAGTGACATATCTGCCTATACATATGAGCGCACCCTGATGATGGAG
  CAGAGGTCCCAGATGCTTCGGCATATGCGGCTGTCCAAAACAGAGCGAGACAGGGAGGCACAGCTGGTGA
  AAGATCGAAACTCAATGCTACGCTTGACCAGCATTGGCTCTGATGAGGACGAAGAGACAGAAACGTACCA
  GGAGAAGGTGCACATGACTTGGACCAAGGATAAATACATGGCATCCCGGGGGCAAAAGGTCAAGTCAATG
  GAAGGATTCCAGGACCTACTTAATATGCGTCCGGACCAGTCCAACGTGAGACGGATGCATACAGCAGTGA
  AGCTCAATGAAGTTATAGTCAACAAGTCTCATGAAGCAAAGCTGGTTTTGTTGAATATGCCAGGACCACC
  CCGGAACCCTGAAGGTGATGAAAACTGC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG211642 representing BC062099
  Red=Cloning site Green=Tags(s)
  
  MHPPEATTKMSSVRFMVTPTKIDDIPGLSDTSPDLSSRSSSRVRFSSRESVPETSRSEPMSELSGATTSL
  ATVALDPSSDRTSNPQDVTEDDGHKKARNAYLNNSNYEEGDEYFDKNLALFEEEMDTRPKVSSLLNRMAN
  YTNLTQGAKEHEEAENITEGKKKPTKSPQMGTFMGVYLPCLQNIFGVILFLRLTWVVGTAGILQAFAIVL
  ICCCCTMLTAISMSAIATNGVVPAGGSYFMISRALGPEFGGAVGLCFYLGTTFAAAMYILGAIEIFLVYI
  VPRAAIFRSDDALKESAAMLNNMRVYGTAFLVLMVLVVFIGVRYVNKFASLFLACVIVSILAIYAGAIKS
  SFAPPHFPVCMLGNRTLSSRHLDICSKTKEVDNMTVPSKLWGFFCNSSQFFNATCDEYFVHNNVISIQGI
  PGLASGIITENLWSNYLPKGEIIEKPSAKSSDVLGNLNHEYVLADITTSFTLLVGIFFPSVTGIMAGSNR
  SGDLKDAQKSIPIGTILAILTTSFVYLSNVVLFGACIEGVVLRDKFGDAVKGNLVVGTLSWPSPWVIVIG
  SFFSTCGAGLQSLTGAPRLLQAIAKDNIIPFLRVFGHSKANGEPTWALLLTAAIAELGILIASLDLVAPI
  LSMFFLMCYLFVNLACALQTLLRTPNWRPRFRYYHWALSFMGMSICLALMFISSWYYAIVAMVIAGMIYK
  YIEYQGAEKEWGDGIRGLSLSAARFALLRLEEGPPHTKNWRPQLLVLLKLDEDLHVKHPRLLTFASQLKA
  GKGLTIVGSVIVGNFLENYGDALAAEQTIKHLMEAEKVKGFCQLVVAAKLKEGISHLIQSCGLGGMKHNT
  VVMGWPNGWRQSEDARAWKTFIGTVRVTTAAHLALLVAKNVSFFPSNVEQFSEGNIDVWWIVHDGGMLML
  LPFLLKQHKVWRKCSIRIFTVAQLEDNSIQMKKDLATFLYHLRIEAEVEVVEMHDSDISAYTYERTLMME
  QRSQMLRHMRLSKTERDREAQLVKDRNSMLRLTSIGSDEDEETETYQEKVHMTWTKDKYMASRGQKVKSM
  EGFQDLLNMRPDQSNVRRMHTAVKLNEVIVNKSHEAKLVLLNMPGPPRNPEGDENC
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC062099
• ORF Size: 3318 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC062099.1
• RefSeq Size: 4439 bp
• RefSeq ORF: 3320 bp
• Locus ID: 107723
• Cytogenetics: 2 E3
• Gene Summary: Mediates electroneutral potassium-chloride cotransport. May be activated by cell swelling. May contribute to cell volume homeostasis in single cells.[UniProtKB/Swiss-Prot Function]