Pigo (NM_020035) Mouse Tagged ORF Clone

CAT#: MG211637

Pigo (tGFP-tagged) - Mouse phosphatidylinositol glycan anchor biosynthesis, class O (Pigo)

Product Images

Circular map for MG211637

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Pigo
• Synonyms: PIG-O
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG211637 representing NM_020035
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACATTCTCTTCTCTCCCAGGATGCGGAAGGTGTCTGTGCTCCTCTTCTTAGCCTGGGTCTGTTTTC
  TTTTCTACGCTGGCATTGCACTCTTCACCAGTGGCTTCCTTCTCACTCGTCTGGAGCTCACCAATCAAAG
  CAGCTGCCAAGAGCTCCCAGGCCCTGGTCCCTTGCCGTGGGGAAGTCATGGAAAGCCTGGGGCTTGCTGG
  ATGCCTTCCCGCTTCTCCAGGGTGGTTTTGGTACTGATAGACGCTCTGCGGTTTGACTTTGCCCAGCCTC
  AGCGCTCGCATGTGCCTGGGGAACCTCCTGTCTCCGTGCCTTTTCTGGGAAAATTAGGTTCCTTGCAGAG
  GATCCTAGAGAGTCAACCCCACCATGGCCGGCTGTACCGATCTCAGGTGGACCCCCCCACGACCACCATG
  CAGCGTCTAAAGGCTCTCACCACTGGCTCGCTGCCGACTTTCATTGATGCTGGCAGTAACTTTGCCAGCC
  ATGCTATAGTGGAAGATAATGTCATCCAACAGCTCAACAGTGCAGGAAGGCGTGTAGTCTTCATGGGAGA
  TGATACCTGGAGAGACCTCTTCCCCGGAGCCTTTTCTCAAGCTTTCTTCTTCTCATCCTTCAATGTCAGA
  GACCTGCACACAGTGGATAACGGCATCTTGGAACACCTCTATCCTACCCTGGACGGCGGTTCGTGGGATG
  TGCTGATCGCTCACTTCCTGGGTGTGGATCACTGTGGTCACAAGCACGGCCCTCACCACCCTGAAATGGC
  CAAGAAACTTAGCCAGATGGACCAGGTGATACAGGGACTCATAGAACGCCTGGAGAATGACACACTGCTG
  GTGGTAGCCGGTGACCATGGGATGACCATGAATGGGGACCATGGAGGGGACAGTGAGCTGGAGGTGTCAG
  CTGCACTTTTCCTGTACAGCCCCACAGCTCTCTTCCCTAGTGTCCCACCCGAGGAGCCGGAGGTTATTCC
  TCAGGTCAGCCTTGTGCCCACGCTGGCACTGCTGCTAGGCCTGCCCATCCCGTTTGGGAACACTGGGGAA
  GTGATGGCAGAGCTATTCTCAGGTGGCAGTGACTCCTCCCATCCTCACTCCTCTGCGCTAGCCCAAGTCT
  CGGCTCTCCACATCAATGCCCAGCAGGTGTCCCGGTTTCTTCATACCTACTCGGCTGCGACTCAGGATCT
  CCAAGTTAAAGAACTTCATCGGCTGCAGACGCTCTTCTCCAAGGCCTCTGCGCGTTACCAGCACTTTCTG
  CGGGATCCCCAAGAGGCTGAGGCAGCGCTGAGCACATTGACTGCTGAGTTCCAGCAGTTCCTGCGGGGAG
  CTCGGGCCCTGTGCATCGAGTCTTGGGCCCGCTTCTCTCTGGTCCGAATGGCAGGGGGCGCTGCTCTTTT
  GGCTGCTGCCTGCCTTCTCTGCCTGCTTGCTTCCCAGTTGGCAGTCGCCCCAGGCTTTCTCTTCCGCCCA
  CTACTGCTAATACCTGTAGCCTGGGGCCTAACTTGGACCATCCTGTATGCTGGAGTCTCGGTAACTACTG
  GGTCGAAGATAGACTTAGTGGTTCTGGGGGCTGTGGCTGCAGCCGGTTCACTCCTCCCTTTTCTGTGGAA
  AGCTTGGGTTAGCAGGGGGTCTAAGAGGCCACTGGCTCCCCTACTTCCTGTCCCTAGACCTGTCCTAATA
  TTACTGCTCATTCGCTTGGCCACCTTCTTCTCGGATAGCTTTGTTGTTGCTGAGGCCAGGGCCACCCCCT
  TCCTTCTGGGCTCTCTCGTTTTCTTCCTGGTTGCTCAGCTCCACTGGGAGGGTCAGCTACTGCCACCCAA
  ACCACTCACGATGTCCCGCCTTGGCTCTTCTGCCCCAACAGCGCCCCCACGGCACAGTGGCGCGCATGCC
  CTGTGGCTTGGAATTGGGTTGCTTTTATTTACAAGGCTCGCTGGGCTTTTTCACCGCTGCCCGGAAGAGA
  CACCTGCCTGTCGCTCCTCTCCCTGGCTGAGTCCTCTGGCATCTATGGTGGGAGGTCGAGCCAAGAATTT
  GTGGTACGGAGCTTGCGTGGGGGCACTGGTGGCCCTGTTAGTCGTCGTGCGCTTGTGGCTTCGCCGCTAT
  GGTAATCTCAAGAGTCCTGAGCCCCCTGTGCTCTTTGTGCGCTGGGGGATGCCTCTCATGGTACTGGGCA
  CGGCTGCCTACTGGGCACTGGCATCAGGTGCAGAGGAAGCACCACCACGCCTCCGGGCCCTGGTCGCCGG
  GGCATCAGCTGTGCTGCCTCGGGCTGTGATGGGGCTGGCTGCCTTGGGGCTCGTGCTGCTGCTCTGGAGG
  CCTGTGACAGTGCTGGTGAAAGCTGGGGCTGCTACTTCAAGGACCAGGACTATCCTCACTCCCTTCTCAG
  GTCCCCCTACTTCTCAGGCTGACCTCGACTATGTAGTCCCACAAATCTACCGCCACATGCAGGAGGAGTT
  CCAGGGCCGGCTCGAGAGGACTAAAGCTCAGGGTCCCATAACCGTGGCTGCCTATCAGTTGGGAAGCGTG
  TACTCGGCTGCTATGGTCACGGCCCTCCTCCTCTTGGCCTTCCCACTTATGCTGTTGCATGTAGAGCGGG
  TCAGCCTGGTGTTCCTGCTCCTGTTTCTGCAGAGCTTCCTTCTCCTGCATCTGCTGGCCGCTGGGACACC
  AGTCGCCACCCCTGGCCCTTTTACTGTGCTGTGGCAGGCAGTCTCAGCTTGGGTCCTTCTGGCTACCCAG
  ACCTTCTACTCCACAGGCCACCAGCCTGTCTTTTCAGCCATCCATTGGCACGCAGCCTTTGTGGGCTTCC
  CAGACGGCCATGGCTCTTCTACCTGGTTGCCTGCCTTACTAGTGGGAGCTAACACCTTTGCCTCCCACCT
  CCTCTTCGCAGTAGGCTGCCCACTGCTCCTGCTCTGGCCCTTCCTGTGTGAGCGTCAAGGGCCAAAGAGA
  AGGCAGCCCCTCCCGGGAAGTGAGTCTGAAGCCAGAGTCCGGCCTGAGGAAGAGGAGGAGCTGCAGGAGC
  CACTGATGGAGATGCGCCTCCGGGATGCTCCGAATCACTTCAATGCAGCCCTGTTGCAGCTGGGCCTCAA
  GTACCTCTTTATCCTTGGAGCTCAGATTCTAGCTTGTGCCTTGGCAGCTTCCATCCTCCGCAGGCACCTC
  ATGGTCTGGAAGGTGTTTGCTCCCAAGTTCATTTTTGAAGCCGTAGGTTTCATCGTGAGCAGTGTGGGAC
  TTCTCCTGGGCATAGCATTGGTGATGCGAGTGGATGTCGCTGTGAGTTCCTGGTTCAAAAAACTAGTTCT
  GGCCCAGCAGAGG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG211637 representing NM_020035
  Red=Cloning site Green=Tags(s)
  
  MNILFSPRMRKVSVLLFLAWVCFLFYAGIALFTSGFLLTRLELTNQSSCQELPGPGPLPWGSHGKPGACW
  MPSRFSRVVLVLIDALRFDFAQPQRSHVPGEPPVSVPFLGKLGSLQRILESQPHHGRLYRSQVDPPTTTM
  QRLKALTTGSLPTFIDAGSNFASHAIVEDNVIQQLNSAGRRVVFMGDDTWRDLFPGAFSQAFFFSSFNVR
  DLHTVDNGILEHLYPTLDGGSWDVLIAHFLGVDHCGHKHGPHHPEMAKKLSQMDQVIQGLIERLENDTLL
  VVAGDHGMTMNGDHGGDSELEVSAALFLYSPTALFPSVPPEEPEVIPQVSLVPTLALLLGLPIPFGNTGE
  VMAELFSGGSDSSHPHSSALAQVSALHINAQQVSRFLHTYSAATQDLQVKELHRLQTLFSKASARYQHFL
  RDPQEAEAALSTLTAEFQQFLRGARALCIESWARFSLVRMAGGAALLAAACLLCLLASQLAVAPGFLFRP
  LLLIPVAWGLTWTILYAGVSVTTGSKIDLVVLGAVAAAGSLLPFLWKAWVSRGSKRPLAPLLPVPRPVLI
  LLLIRLATFFSDSFVVAEARATPFLLGSLVFFLVAQLHWEGQLLPPKPLTMSRLGSSAPTAPPRHSGAHA
  LWLGIGLLLFTRLAGLFHRCPEETPACRSSPWLSPLASMVGGRAKNLWYGACVGALVALLVVVRLWLRRY
  GNLKSPEPPVLFVRWGMPLMVLGTAAYWALASGAEEAPPRLRALVAGASAVLPRAVMGLAALGLVLLLWR
  PVTVLVKAGAATSRTRTILTPFSGPPTSQADLDYVVPQIYRHMQEEFQGRLERTKAQGPITVAAYQLGSV
  YSAAMVTALLLLAFPLMLLHVERVSLVFLLLFLQSFLLLHLLAAGTPVATPGPFTVLWQAVSAWVLLATQ
  TFYSTGHQPVFSAIHWHAAFVGFPDGHGSSTWLPALLVGANTFASHLLFAVGCPLLLLWPFLCERQGPKR
  RQPLPGSESEARVRPEEEEELQEPLMEMRLRDAPNHFNAALLQLGLKYLFILGAQILACALAASILRRHL
  MVWKVFAPKFIFEAVGFIVSSVGLLLGIALVMRVDVAVSSWFKKLVLAQQR
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_020035
• ORF Size: 3303 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_020035.3, NP_064419.2
• RefSeq Size: 4261 bp
• RefSeq ORF: 3306 bp
• Locus ID: 56703
• Cytogenetics: 4 A5