Pcdh1 (NM_029357) Mouse Tagged ORF Clone

CAT#: MG211504

Pcdh1 (tGFP-tagged) - Mouse protocadherin 1 (Pcdh1)

Product Images

Circular map for MG211504

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Pcdh1
• Synonyms: 2010005A06Rik; AI585920
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG211504 representing NM_029357
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGCCTCTGAGGCCCAGCCCAGGGCCTGGGGGGCAGCGGTTGCTGCTGCCCCCCTTACTGCTGGCAC
  TGCTGCTCCTATTGGCTCCATCTGCCAGCCATACCACCCAGGTAGTATACAAGGTGCCAGAGGAACAGCC
  GCCCAACACCCTTATTGGGAGCCTTGCTGCCGACTACGGTTTACCAGACGTGGGTCATCTGTATAAACTA
  GAGGTAGGTGCTCCGTATCTTCGAGTGGATGGCAAGACTGGAGACATTTTCACCACAGAGACCTCCATTG
  ATCGAGAGGGGCTCCGTGAATGCCAGAACCAGATCCCTGGGAATCCCTGTATCCTGGAGTTTGAGGTGTC
  AATCACGGACCTCGTGCAGAACGGCAGCCCCCGGCTGCTAGAGGGCCAGATAGAGGTGCAAGACATCAAT
  GACAACACGCCGAACTTTGCCTCGCCAGTCATTACCCTGGCCATCCCTGAGAACACCAACATCGGCTCGC
  TCTTCCCCATCCCACTGGCCACAGACCGTGACGCCGGCCCCAATGGCGTAGCATCCTATGAGCTGCAGGC
  TGGGCCTGAGGCCCAGGAGCTATTTGGACTACAGGTAGCCGAGGACCAGGAGGAGAAGCAGCCACAGCTC
  ATTGTCATGGGCAACCTGGACCGTGAACGCTGGGACTCCTACGACCTCACCATCAAGGTACAGGATGGAG
  GCAATCCTCCACGTGCCAGCAGCGCCCTGCTTCGTGTCACTGTGCTTGATACAAACGACAACGCCCCCAA
  ATTCGAGCGGCCTACCTACGAAGCCGAGCTGTCTGAGAACAGTCCCATTGGCCACTCGGTCATCCAGGTG
  AAAGCTAACGACTCAGACCAAGGTGCCAATGCGGAGATTGACTACACGTTCCACCAGGCACCTGAGGTTG
  TCAGGCGCCTTTTGCGACTAGACCGAAACACTGGACTTATCACCGTCCAGGGCCCCGTGGACCGAGAGGA
  TTTAAGCACTCTGCGTTTCTCAGTGCTCGCCAAGGACCGAGGCGCCACCCCCAAGAGTGCCCGTGCCCAG
  GTAGTTGTGACTGTGAAAGACATGAACGACAATGCCCCGACCATCGAGATCCGAGGCATAGGGCTGGTGA
  CTCATCAAGACGGAATGGCTAACATATCAGAGGACGTGGCTGAGGAGACCGCCGTGGCCTTGGTGCAGGT
  GTCTGATCGAGACGAGGGAGAGAACGCAGCCGTCACCTGTGTCGTAGCAGGCGATGTGCCCTTCCAGCTA
  CGCCAGGCCAGCGAGACAGGCAGCGACAGCAAGAAAAAGTATTTCCTGCAGACCACCACCCCTCTCGACT
  ATGAGAAAGTCAAAGACTATACCATCGAGATCGTGGCTGTGGATTCCGGCAACCCTCCACTCTCTAGCAC
  CAACTCCCTCAAGGTGCAGGTGGTAGACGTCAATGACAACGCCCCCGTCTTCACCCAGAGCATCACTGAA
  GTTGCCTTCCCAGAAAACAACAAGCCTGGTGAAGTGGTGGCCGAGGTCACGGCCAGTGATGCTGACTCTG
  GCTCTAACGCAGAGCTAGTATACTCGCTGGAACCTGAGCCAGCTGCCCAAGGGCTCTTCACTATCTCACC
  CGAGAATGGAGAGATCCGGGTGAAGACATCACTGGATCGGGAACAGAGAGACAGCTATGAGCTGAAGGTG
  GTTGCGGCCGACAGGGGCAGTCCCAGCCTTCAGGGCACAGCCACAGTCCTCGTCAATGTGCTCGACTGTA
  ATGACAATGACCCCAAATTTATGCTGAGTGGCTACAACTTCTCAGTGATGGAGAATATGCCAGCACTGAG
  TCCAGTGGGTATGGTGACTGTCATTGACGGAGACAAAGGGGAGAATGCCCGGGTGCAGCTCTCGGTAGAG
  CAGGACAATGGTGACTTTGTTATCCAGAATGGCACAGGCACCATCTTATCCAGTTTGAGCTTCGATCGGG
  AGCAACAGAGTACCTACACCTTCCAACTGAAGGCCGTAGATGGCGGAGTCCCACCTCGTTCGGCTTATGT
  TGGTGTCACCATCAATGTGCTGGATGAGAATGACAATGCACCCTTTATCACCGCCCCCTCCAACACATCT
  CACCGACTGCTGACCCCGCAGACACGTCTTGGTGAGACAGTCAGCCAGGTGACAGCGGAGGACATTGACT
  CTGGTATCAATGCTGAGCTGACCTATAGCATCGCTGGCGGCAACCCTTATGGGCTCTTCCAGATTGGATC
  ACATTCGGGAGCCATCACGCTGGAAAAAGAAATTGAACGGCGCCACCACGGGTTACACCGCCTCGTGGTG
  AAGGTCAGTGACCGTGGCAAGCCCCCTCGCTACGGCACAGCCTTAGTCCACCTTTACGTCAACGAGACCT
  TAGCCAACCGCACGCTGCTAGAGACCCTCCTCGTCCATAGCCTGGACACGCCCCTGGATATTGACATCGC
  TGGGGATCCGGAATACGAGCGTTCCAAGCAGCGTGGCAACATTCTCTTTGGGGTTGTAGCCGGTGTGGTG
  GCCGTCGCCTTACTCATAGCCCTGGCAGTACTAGTGCGCTACTGCCGGCAGCGGGAGGCCAAGAGCGGCT
  ACCAGGCTGGTAAGAAGGAGACTAAGGACCTGTATGCCCCCAAGCCTAGCGGCAAAGCTGCTAAGGGAAG
  CAAGAGCAAAGGGAAGAAAAGCAAGTCACCGAAACCTGTGAAGCCAGTGGAGGATGAAGATGACACCGGG
  CTGCAGAAGTCCCTCAAGTTCAACCTAATGAGCGATGCCCCTGGAGACAGTCCCCGAATCCACCTGCCCC
  TCAACTACCCACCAGGCAGCCCCGACTTGGGCCGCCATTACCGCTCCAACTCCCCACTGCCTTCTATCCA
  GCTGCAACCCCAGTCACCCTCGGCCTCCAAGAAGCACCAGGTGGTGCAGGACCTGCCGCCTGCAAACACA
  TTTGTGGGCACTGGGGACACCACGTCCACGGGCTCCGAGCAGTACTCCGACTACAGCTATCGCACCAACC
  CCCCCAAATACCCCAGCAAGCAGGTAGGCCAGCCCTTTCGGCTTAGCACGCCCCAGCCCCCACCCCACCC
  CTACCACGGGGCCATCTGGACCGAGGTGTGGGAA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG211504 representing NM_029357
  Red=Cloning site Green=Tags(s)
  
  MGPLRPSPGPGGQRLLLPPLLLALLLLLAPSASHTTQVVYKVPEEQPPNTLIGSLAADYGLPDVGHLYKL
  EVGAPYLRVDGKTGDIFTTETSIDREGLRECQNQIPGNPCILEFEVSITDLVQNGSPRLLEGQIEVQDIN
  DNTPNFASPVITLAIPENTNIGSLFPIPLATDRDAGPNGVASYELQAGPEAQELFGLQVAEDQEEKQPQL
  IVMGNLDRERWDSYDLTIKVQDGGNPPRASSALLRVTVLDTNDNAPKFERPTYEAELSENSPIGHSVIQV
  KANDSDQGANAEIDYTFHQAPEVVRRLLRLDRNTGLITVQGPVDREDLSTLRFSVLAKDRGATPKSARAQ
  VVVTVKDMNDNAPTIEIRGIGLVTHQDGMANISEDVAEETAVALVQVSDRDEGENAAVTCVVAGDVPFQL
  RQASETGSDSKKKYFLQTTTPLDYEKVKDYTIEIVAVDSGNPPLSSTNSLKVQVVDVNDNAPVFTQSITE
  VAFPENNKPGEVVAEVTASDADSGSNAELVYSLEPEPAAQGLFTISPENGEIRVKTSLDREQRDSYELKV
  VAADRGSPSLQGTATVLVNVLDCNDNDPKFMLSGYNFSVMENMPALSPVGMVTVIDGDKGENARVQLSVE
  QDNGDFVIQNGTGTILSSLSFDREQQSTYTFQLKAVDGGVPPRSAYVGVTINVLDENDNAPFITAPSNTS
  HRLLTPQTRLGETVSQVTAEDIDSGINAELTYSIAGGNPYGLFQIGSHSGAITLEKEIERRHHGLHRLVV
  KVSDRGKPPRYGTALVHLYVNETLANRTLLETLLVHSLDTPLDIDIAGDPEYERSKQRGNILFGVVAGVV
  AVALLIALAVLVRYCRQREAKSGYQAGKKETKDLYAPKPSGKAAKGSKSKGKKSKSPKPVKPVEDEDDTG
  LQKSLKFNLMSDAPGDSPRIHLPLNYPPGSPDLGRHYRSNSPLPSIQLQPQSPSASKKHQVVQDLPPANT
  FVGTGDTTSTGSEQYSDYSYRTNPPKYPSKQVGQPFRLSTPQPPPHPYHGAIWTEVWE
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_029357
• ORF Size: 3114 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_029357.2
• RefSeq Size: 3902 bp
• RefSeq ORF: 3117 bp
• Locus ID: 75599
• Cytogenetics: 18 20.08 cM