Senp7 (BC058593) Mouse Tagged ORF Clone

CAT#: MG211497

Senp7 (tGFP-tagged) - Mouse SUMO1/sentrin specific peptidase 7 (cDNA clone MGC:67838 IMAGE:5364345)

Product Images

Circular map for MG211497

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Senp7
• Synonyms: mKIAA1707
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG211497 representing BC058593
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACAGGGCCAGGCCTGGGCGTCGGCGAGCTTCATCAGAGATTGTTACAGAGGGAAAACGGAAAAAGT
  CATCACCAGCTGATTTACAGAAGATAACAAAGTTGTTAACTGTAAAGTCAGAGGATGTTCTTGCTCAGTC
  ACCATTGTCCAAACTCAGAGGCTCAGAATGCTGGTGGACAAGAAGCCTAAGAAATAAAGTCATCTGTCTA
  GACCACAAAAAACCAAAAGCTGCCCGTGGGTGTCCTCCTAAGGGATTACCAAAAAGGCATCTCAGAGTTA
  TGTTGACGAATGTTCTATGGACGGACTTAGGACGAGAATTCAGAAAGACCCTGCCTAGAAAGGATGCTAA
  TTTATGTGCTCCCAGCAAGGTGCAATCAGACTCATTGCCTTCGACATCTGTTGACAGCATAGAGACATGT
  CAAAGATTAGATCCTCTTCACCAAAGCCTTAATTTATCCGAAAGGACACCCAGAGTTATACTGACGGATA
  TCCGGCAAACAGAATTAGGAAGAAAATATTTAAAGATCCCACCTGTAACTGAGGCCAGTTTGAGTGATAC
  AGCCAACCTGAAATCAGAGCAACTTTCTTCATCATCTGATGGCAGCTTAGAGTCTTGTCAGAGTGTAAAT
  CATCACAAGAGCTTTTTATCTGAAAGTGGTCCCAAACCAAGTAGGACAGGTGACGTTCCTGCAAAGGAGG
  CTGCATGTGGGGGACAGAAGCAGGGTGATGATGGAGGAGTCACTCCTGAGATGGCTGCTCCTCATCCTAA
  AGACTTTAATACTGGAAATAAAGGTTGTGATTATCTTGAAGAGGGGACCAGCAACAAGAATACTTCATAT
  TCTTATTCGGAAATGGACCATACTCCAGTTTCCAGGAAGAGAAAGAAAAGGGGTAGAAGTAATTTTCATG
  ATTCTCATAATTCTAAGACTTCTTTGGATAAGCCAACAGAACATACAAAAGAAGAAGAAAATGACTCTTC
  AGTCTCCCGTAAGCTTGAAGAGTCCGGTGAAGACTCTCACCAGGACCCAGCACCACCCGAAGGACTCGCG
  CCAGAATCTTTAGAGAGCGAGGCCACTAACCTGCGCTCATTCGCTGCTGGTCAGGAGCCAGATGCTAGCG
  CTGCCTCTGGAAGGGCCTCTTCACCCAACAAAAGCTTGGAGAGCTCTGCTTCTAGTGAAGTGTCGGAGAA
  TTCTTCCGTGGCTGTGAAGGGTGAGGCGTTGACTCTAAAAGAAGCATCCCCTCCGGGTGGAAGCAGTGAA
  GAGAGCCAGTTGTTGATCTCAGCTGAGCCAATTGTAGTTTCCAGTGATGAAGAAGGACCGGTTGAACATA
  AGAATTCAGTAATTCTAAAATTACAGCCTCCTCATGAGATTATGAGTGAAAATCAGGGCACTTCTGACCC
  ACAGTTGTCAGAGCTGACACTCGGTGCATGTGAATCTGTACAGGTAACATCTGAATTATTTCCATACAAT
  CCTGATGTAGAAAACATTTCCTGTATTAAATCTAATAGTGAAATGGATCTGAAATTGGATTTCATATTTA
  CATGTGTGTATATTGGTAAAATAAAAGGAACTCCAAAAGGTTGTGTCACGTTCACAAAGAAGTATATTAA
  AATCCCGTTTCAAGTGTCCACAAATGAGATTTCCCTGACAGTGGACACCGCACGTTTAAAGAGGTTCGGG
  TTATGGGAAAGCAAGGATGAGGACCACAGCAAGAGGAGCCACGCTATCCTTTTCCTCTGGCTTTCCTCAG
  ACTACCTTCAAGACATTCAGACCCAGCTAGAAAACCCCATGCTAAGCCAGCAATCAAAAGCCAATGAATT
  TATTTTCCTTGAGTTAAACAGTTCCATTTCACAAAGAGAAGAATTGAAATTGAAAGATATTATGATGGAA
  ATCAGTACAAAAAATGGAAATTTACACCTGTCTTGTCCATTGCCCTGGGTCCAGGCACTTCCTTTGTTTC
  AGGATCTCTCTCCACAAGAAATCTCTTTTCTTCACTATTACTATGCTTCAGCTTCTGCTCTCCCCACTGC
  TGCTGGGGCAGACATGAAGAAGAAGTCAGTGTCTCAGCCCTCAAATTCAGACACCATCAAGCCTACTTAC
  ACCTTCCTACATAAACAAAGTAGTGGTTGCTACTCGCTCTCTATTACTTCAAGTCCAGAGGAGGAATGGC
  AGGAAGTCAGGAACACGGGACCCGTTCAGAAGTTGATTGTTTATCCTCCACCACCTACTAAGGGAGGCTT
  GGGAGTAACTAATGAAGATCTAGAGTGTTTAGAAGAAGGAGAATTTCTTAACGATGTAATCATTGACTTT
  TACCTTAAGTATCTTCTATTGGAGAAGGCATCAGATGAACTTGTTGAACGAAGTCACATTTTTAGTAGCT
  TTTTCTATAAATGCTTGACAAGAAAGGAAAATAATTTAACAGAAGATAATCCAGATCTATCAGTGGCTCA
  GAGGAGACACCGAAGAGTGAGAACATGGACTCGTCATATAAACATTTTTAATAAAGACTACATCTTTGTA
  CCTGTTAATGAATCGTCTCACTGGTATCTTGCAGTCATTTGTTTCCCATGGCTAGAAGAGGCTGTGTATG
  AAGATTGTCCACAAACTGTGTCCCAACAGTTCCAGGGTCAGCAGTCTCAGCATGACCACAAAATGACAGA
  TAATGATCCACATACCACTTCAACTGTGTCCACGAGTGCAGAGGATTCCCAAAGTACCGAGGTGAATATG
  TCAGTACCAAAGAAAATGTGTAAGCGGCCATGTATTCTCATACTAGACTCCCTGAAAGCTGCTTCTATAC
  AAAACACAGTTCAGAACTTACGAGAGTATTTGGAGGTAGAGTGGGAGGTGAAACGAAAAACGCACCGTGA
  ATTCAGTAAAACCAATATGGTCGACCTGTGCCCCAAAGTCCCTAAGCAGGATAACAGCAGTGACTGTGGA
  GTCTATTTGTTGCAGTATGTGGAGAGCTTTTTCCAGGATCCCATTGTTAACTTTGAACTTCCAATTCACT
  TGGAGAAATGGTTTCCTCGCCATGTAATAAAGACCAAACGGGAAGATATTCGAGAGCTCATTTTGAAACT
  TCACTTACAGCAACAGAAGGGCGGCAGCTGC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG211497 representing BC058593
  Red=Cloning site Green=Tags(s)
  
  MDRARPGRRRASSEIVTEGKRKKSSPADLQKITKLLTVKSEDVLAQSPLSKLRGSECWWTRSLRNKVICL
  DHKKPKAARGCPPKGLPKRHLRVMLTNVLWTDLGREFRKTLPRKDANLCAPSKVQSDSLPSTSVDSIETC
  QRLDPLHQSLNLSERTPRVILTDIRQTELGRKYLKIPPVTEASLSDTANLKSEQLSSSSDGSLESCQSVN
  HHKSFLSESGPKPSRTGDVPAKEAACGGQKQGDDGGVTPEMAAPHPKDFNTGNKGCDYLEEGTSNKNTSY
  SYSEMDHTPVSRKRKKRGRSNFHDSHNSKTSLDKPTEHTKEEENDSSVSRKLEESGEDSHQDPAPPEGLA
  PESLESEATNLRSFAAGQEPDASAASGRASSPNKSLESSASSEVSENSSVAVKGEALTLKEASPPGGSSE
  ESQLLISAEPIVVSSDEEGPVEHKNSVILKLQPPHEIMSENQGTSDPQLSELTLGACESVQVTSELFPYN
  PDVENISCIKSNSEMDLKLDFIFTCVYIGKIKGTPKGCVTFTKKYIKIPFQVSTNEISLTVDTARLKRFG
  LWESKDEDHSKRSHAILFLWLSSDYLQDIQTQLENPMLSQQSKANEFIFLELNSSISQREELKLKDIMME
  ISTKNGNLHLSCPLPWVQALPLFQDLSPQEISFLHYYYASASALPTAAGADMKKKSVSQPSNSDTIKPTY
  TFLHKQSSGCYSLSITSSPEEEWQEVRNTGPVQKLIVYPPPPTKGGLGVTNEDLECLEEGEFLNDVIIDF
  YLKYLLLEKASDELVERSHIFSSFFYKCLTRKENNLTEDNPDLSVAQRRHRRVRTWTRHINIFNKDYIFV
  PVNESSHWYLAVICFPWLEEAVYEDCPQTVSQQFQGQQSQHDHKMTDNDPHTTSTVSTSAEDSQSTEVNM
  SVPKKMCKRPCILILDSLKAASIQNTVQNLREYLEVEWEVKRKTHREFSKTNMVDLCPKVPKQDNSSDCG
  VYLLQYVESFFQDPIVNFELPIHLEKWFPRHVIKTKREDIRELILKLHLQQQKGGSC
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC058593
• ORF Size: 3113 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC058593, AAH58593
• RefSeq Size: 4788 bp
• RefSeq ORF: 3113 bp
• Locus ID: 66315
• Cytogenetics: 16 C1.1
• Gene Summary: This gene encodes a SUMO deconjugating enzyme of the Sentrin/SUMO-specific protease (SENP) family. The encoded protein is a protease that exhibits deSUMOylating activity towards proteins involved in chromatin remodeling and promotes chromatin relaxation for DNA repair or transcription. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Jan 2015]