Sema6d (BC060680) Mouse Tagged ORF Clone

CAT#: MG211412

Sema6d (tGFP-tagged) - Mouse sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (cDNA clone MGC:66587

Product Images

Circular map for MG211412

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Sema6d
• Synonyms: NM_199238, Sema6D-1, Sema6D-2, Sema6D-4, Sema6D-5, Sema6D-6, mKIAA1479, D330011G23
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG211412 representing BC060680
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGTTCCTTCTGCTTTGGTTCTGCGTGCTGTTCCTTCTGGTCTCCAGGTTACGGGCGGTCAGCTTCC
  CAGAAGACGATGAGCCCCTCAACACGGTTGACTATCACTATTCAAGGCAATATCCGGTTTTTAGAGGACG
  CCCTTCAGGCAACGAATCGCAGCACAGGCTGGACTTTCAGCTGATGTTGAAAATTCGAGACACACTTTAT
  ATTGCTGGCAGGGATCAAGTCTATACAGTGAACTTAAATGAAATCCCCCAAACAGAGGTGATACCAAGCA
  AGAAGCTGACGTGGAGGTCCAGACAGCAGGATCGAGAAAATTGTGCTATGAAAGGCAAGCATAAAGATGA
  ATGCCACAACTTCATCAAAGTCTTTGTCCCAAGAAATGATGAGATGGTTTTTGTCTGTGGTACCAATGCT
  TTCAACCCGATGTGCAGATACTATAGGTTGAGAACGTTAGAGTATGATGGGGAAGAAATTAGTGGCCTGG
  CACGATGCCCGTTTGATGCCCGACAAACCAATGTCGCCCTCTTTGCTGATGGAAAACTCTATTCTGCCAC
  AGTGGCTGATTTCCTGGCCAGTGATGCTGTCATTTACAGAAGCATGGGAGATGGATCTGCCCTTCGCACA
  ATAAAATACGATTCCAAGTGGATCAAAGAACCACACTTCCTTCATGCCATAGAATATGGAAACTATGTCT
  ATTTCTTCTTCAGAGAAATCGCCGTGGAACATAATAACTTAGGCAAGGCTGTGTATTCCCGCGTGGCTCG
  CATTTGTAAAAACGACATGGGTGGCTCACAGCGGGTCCTGGAGAAACACTGGACTTCCTTCCTTAAGGCT
  CGGCTGAACTGCTCCGTTCCTGGAGATTCCTTTTTCTACTTCGACGTCCTGCAGTCTATAACAGACATAA
  TCCAAATCAATGGCATCCCCACTGTGGTTGGGGTCTTCACCACACAGCTCAACAGCATTCCTGGTTCTGC
  AGTCTGTGCCTTTAGCATGGACGACATTGAGAAAGTGTTCAAAGGGCGGTTCAAAGAGCAGAAAACCCCA
  GACTCTGTTTGGACAGCAGTTCCCGAAGACAAAGTACCAAAACCAAGGCCTGGCTGTTGTGCCAAACACG
  GCCTCGCAGAAGCTTACAAGACCTCCATCGACTTTCCAGATGACACCCTGGCTTTCATCAAGTCCCACCC
  GCTGATGGACTCTGCCGTCCCACCCATTGCCGATGAGCCCTGGTTCACAAAGACACGGGTCAGGTACAGG
  TTGACAGCCATCGAAGTGGACCGTTCAGCAGGGCCATACCAAAACTACACAGTCATCTTTGTTGGCTCTG
  AAGCTGGCGTGGTACTTAAAGTTTTGGCAAAGACCAGTCCTTTCTCTCTGAATGACAGTGTATTACTCGA
  AGAGATTGAAGCTTATAACCCAGCCAAGTGCAGCGCCGAGAGTGAGGAGGACAGAAAGGTGGTCTCATTA
  CAGCTGGACAAGGATCACCATGCTTTATACGTGGCCTTCTCTAGCTGCGTGGTCCGCATCCCCCTCAGCC
  GCTGTGAGCGCTACGGATCGTGTAAAAAGTCTTGCATTGCATCACGTGACCCGTACTGTGGTTGGTTAAG
  CCAGGGAGTTTGTGAGAGAGTGACCCTAGGGATGCTCCCTGGAGGATATGAGCAGGACACGGAGTACGGC
  AACACAGCCCACCTAGGGGACTGCCACGGTGTACGGTGGGAAGTCCAGTCTGGAGAATCCAATCAGATGG
  TCCACATGAATGTCCTCATCACCTGCGTGTTTGCCGCTTTTGTCTTGGGCGCGTTCATCGCAGGAGTGGC
  CGTGTACTGCTACCGTGACATGTTCGTTCGGAAGAACAGAAAGATCCATAAAGACGCAGAATCCGCCCAG
  TCGTGCACAGACTCCAGCGGAAGCTTCGCCAAGCTGAACGGCCTCTTTGACAGCCCCGTCAAGGAATACC
  AGCAGAACATTGATTCTCCCAAACTCTACAGCAACCTGCTGACCAGTCGGAAGGAACTGCCACCAAACAC
  GGATACAAAGTCCATGGCCGTGGACCACAGAGGCCAGCCTCCCGAGCTGGCTGCTCTCCCCACGCCGGAA
  TCCACACCTGTCCTCCACCAGAAGACCCTGCAGGCCATGAAGAGCCACTCTGAGAAGGCCCACAGCCACG
  GTGCTTCAAGGAAAGAACACCCCCAGTTTTTTCCTTCTAGTCCTCCACCCCATTCCCCATTGAGTCACGG
  GCATATCCCCAGTGCCATCGTTCTTCCAAACGCCACTCACGACTACAATACATCCTTCTCCAACTCGAAT
  GCCCACAAAGCCGAAAAGAAGCTTCAGAGCATGGATCACCCTCTTACGAAGTCATCCAGTAAGCGGGAGC
  ACCGGCGGTCTGTGGATTCCAGGAATACTCTCAATGATCTCCTGAAGCATCTAAATGACCCAAACAGTAA
  CCCCAAAGCCATCCTGGGAGAGATCCATATGGCTCATCAAACCCTCATGCTGGACCCGGTGGGACCAATG
  GCTGAGGTCCCACCCAAGGTCCCTAACCGGGAGGCATCTCTATACTCCCCTCCCTCCACACTCCCCAGAA
  ATAGTCCAACCAAGAGAGTAGATGTCCCCACCACTCCTGGGGTGCCAATGACTTCTCTGGAAAGACAAAG
  GGGTTATCACAAAAATTCCTCCCAGAGGCACTCTATATCTGCCGTGCCTAAAAACTTAAACTCACCAAAT
  GGTGTTTTGTTATCTAGACAGCCGAGTATGAACCGTGGAGGCTATATGCCCACCCCAACAGGGGCGAAGG
  TGGACTATATTCAGGGGACACCGGTGAGTGTTCATCTGCAGCCCTCCCTCTCCAGACAGAGCAGCTATAC
  CAGTAATGGCACCCTCCCCAGGACGGGACTAAAGAGGACACCATCCTTAAAACCTGATGTGCCACCAAAG
  CCTTCCTTTGTTCCGCAAACCACATCTGTCAGACCACTGAACAAGTACACGTAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG211412 representing BC060680
  Red=Cloning site Green=Tags(s)
  
  MGFLLLWFCVLFLLVSRLRAVSFPEDDEPLNTVDYHYSRQYPVFRGRPSGNESQHRLDFQLMLKIRDTLY
  IAGRDQVYTVNLNEIPQTEVIPSKKLTWRSRQQDRENCAMKGKHKDECHNFIKVFVPRNDEMVFVCGTNA
  FNPMCRYYRLRTLEYDGEEISGLARCPFDARQTNVALFADGKLYSATVADFLASDAVIYRSMGDGSALRT
  IKYDSKWIKEPHFLHAIEYGNYVYFFFREIAVEHNNLGKAVYSRVARICKNDMGGSQRVLEKHWTSFLKA
  RLNCSVPGDSFFYFDVLQSITDIIQINGIPTVVGVFTTQLNSIPGSAVCAFSMDDIEKVFKGRFKEQKTP
  DSVWTAVPEDKVPKPRPGCCAKHGLAEAYKTSIDFPDDTLAFIKSHPLMDSAVPPIADEPWFTKTRVRYR
  LTAIEVDRSAGPYQNYTVIFVGSEAGVVLKVLAKTSPFSLNDSVLLEEIEAYNPAKCSAESEEDRKVVSL
  QLDKDHHALYVAFSSCVVRIPLSRCERYGSCKKSCIASRDPYCGWLSQGVCERVTLGMLPGGYEQDTEYG
  NTAHLGDCHGVRWEVQSGESNQMVHMNVLITCVFAAFVLGAFIAGVAVYCYRDMFVRKNRKIHKDAESAQ
  SCTDSSGSFAKLNGLFDSPVKEYQQNIDSPKLYSNLLTSRKELPPNTDTKSMAVDHRGQPPELAALPTPE
  STPVLHQKTLQAMKSHSEKAHSHGASRKEHPQFFPSSPPPHSPLSHGHIPSAIVLPNATHDYNTSFSNSN
  AHKAEKKLQSMDHPLTKSSSKREHRRSVDSRNTLNDLLKHLNDPNSNPKAILGEIHMAHQTLMLDPVGPM
  AEVPPKVPNREASLYSPPSTLPRNSPTKRVDVPTTPGVPMTSLERQRGYHKNSSQRHSISAVPKNLNSPN
  GVLLSRQPSMNRGGYMPTPTGAKVDYIQGTPVSVHLQPSLSRQSSYTSNGTLPRTGLKRTPSLKPDVPPK
  PSFVPQTTSVRPLNKYTY
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC060680
• ORF Size: 2996 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC060680, AAH60680
• RefSeq Size: 5596 bp
• RefSeq ORF: 2996 bp
• Locus ID: 214968
• Cytogenetics: 2 F1
• Gene Summary: Shows growth cone collapsing activity on dorsal root ganglion (DRG) neurons in vitro. May be a stop signal for the DRG neurons in their target areas, and possibly also for other neurons. May also be involved in the maintenance and remodeling of neuronal connections (By similarity).[UniProtKB/Swiss-Prot Function]