Wdr36 (BC094453) Mouse Tagged ORF Clone

CAT#: MG211095

Wdr36 (tGFP-tagged) - Mouse WD repeat domain 36 (cDNA clone MGC:103340 IMAGE:5068941)

Product Images

Circular map for MG211095

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Wdr36
• Synonyms: Ta-wdrp, MGC38421
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG211095 representing BC094453
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGAGATGGAGAGCGCCGTGGAAGGCCGCACGGCCAGCGTGCTGTTCGCGGGGTTCCGGGCCTTGG
  GGCTGTTCAGCAACGAGGTTCCACACGTGGTGCGGTACAGCGCGCTCAAGCGCCGCTTCTACGTGACGAC
  TTGCGTGGGCAAGAGCTTCCACACATATGACGTTCAGAAACTTAGTCTGGTTGCAGTAAGTAACTCTGTT
  CCACAGGATATCTGTTGCATGGCAGCCGATGGGAGGCTAGTCTTCGCTGCATATGGGAACGTTTTCTCTG
  CATTTGCCCGGAATAAAGAGATAGTACATACTTTTAAGGGCCATAAGGCAGAAATACATCTTTTGCAACC
  CTTTGGGGATCACGTTATCTCAGTGGATACTGACAGCGTTCTTATTATTTGGCACATTTATTCAGAAGAA
  GAATACCTTCAGCTGACTTTTGACAAGTCAGTTTTTAAAATCTCTACAATTTTGCATCCAAGCACCTATT
  TGAATAAAGTCCTACTGGGCAGTGAGCAGGGAAGCCTACAGTTATGGAATATAAAGTCCAATAAGCTTCT
  ATATACATTTCCAGGGTGGAAAGTTGGAGTGACAGCTCTTCAACAGGCACCGGCTGTGGATGTTGTTGCC
  ATTGGTCTTGTGTCTGGTCAAGTTGTCATTCACAACATTAAGTATGATGAAACATTAATGAAGTTCCATC
  AGGACTGGGGACCCATTACTTCCATTTCATTTCGCACAGATGGTCATCCAATAATGGCAGCTGGAAGCCC
  GTGTGGCCATATTGGACTCTGGGATCTGGAAGACAAAAAATTAATTAATCAAATGAGAAATGCACACTCT
  ACAGCAATATCTGGGCTGACATTTCTCCATAGAGAGCCGTTGCTTGTTACAAATGGCGCTGATAATGCTC
  TTAGGATATGGATCTTTGATGGCCCCGCGGGTGAAGGACGGCTGTTGAGATTCAGAATGGGGCATAGTGC
  TTCTCTTACCAAGATTAGATATTATGGACAAAATGGACAGCAGATTTTAAGTGCAAGTCAAGATGGGACT
  CTACAGTCATTTTCTACAGTTCATGAAAAATTCAACAAGAGCTTGGGTCATGGATTAGTAAACAAAAAGA
  TAGTTAAACGAAAAGGACTTCAGAACACCATGTCAGTAAGGCTCCCACCTATCACACAGTTTGCTGCTGA
  GGAAGCTCGTCAAAGTGACTGGGATGGTATCATTGCTTGCCATCAAGGGAAGCGGTCTTGCTCGACCTGG
  AATTACCAAAGGTCTACAATAGGTGCTTATTTTCTCAAGCCCAGAGGTGTAAAGACAAATAGCAACCTCA
  CTGCAACAGCAGTAGATATAACTTCCTGTGGAAACTTTGCAATAATTGGCCTCTCTTCAGGAGCTGTAGA
  TGTGTATAACATGCAGTCTGGTATACATCGAGGAAATTTTGGAGATGATAAAGCTCATACTGGCTCTGTT
  AGAGGTGTTGCGGTGGATGGATTAAACCAGCTGGTGGTTACAGCTGGAAGTGAACGGTTACTCAAATTCT
  GGAACTTTAAAAGCAAAGTTTTGATCCACTCTCTGGGCCTTGATTCGTCTCCAAATATGATGCTGCTACA
  CAGGGACAGCGGCATCCTGGGCCTTGCTATGGATGACTTCTCCATTGCTGTCCTCGACATAGAGACTAGG
  AAGATCGTCAGAGAGTTTTCTGGACACCACGGCCAAATAAATGACATGACGTTCAGTCCTGATGGCCGCT
  GGTTGATAAGCGCTGCGATGGATTGCTCTGTTAGGACTTGGGACCTTCCTTCTGGGTGCCTTATCGACTG
  CTTTTTGTTGGACTCAGCGCCTCTAAATGTTACTATGTCCCCAACTGGAGACTTTCTAGCAACTTCCCAT
  GTGGATCACCTTGGAATTTATCTATGGTCGAATATTTCCCTCTATTCAGTTGTCTCCCTGCGACCACTTC
  CTCCAGATTATGTCCCTTCCATAGTGATGCTTCCTGGTACTTGTCAAACCCAAGGTATGGAAGACCTGGA
  AGAAAAGACTGAGCCAAGTGATGAAATGATAGAGTATGAGTCGCCAGAACAGTTGAGTGAGCAACTGGTG
  ACTCTCTCCCTTCTCCCTGAGTCACGCTGGAAAAACCTTCTGAATCTTGATGTTATCAAGAAAAAGAATA
  AACCCAAAGAGCCGCCCAAGGTTCCCCAGTCAGCTCCGTTTTTCATCCCAACAGTTCCTGGGCTTGTCCC
  CAGGTTTGCTGTACCCGAACCCAGCAGTGACCCCCAGCAGTCTAAGGTGGTAAACCTTGGGATTTTGGCT
  CAAAAATCAAATTTCTACCTGAAACTTGAAGAAGGATTACTAAATAATCAGTATGAAGCTGCTCTTAACC
  TTCTGAAAGAATTAGGCCCATCTGGGATTGAAACAGAGCTGCGAAACCTGTCTCCTGATGACGGTGGGTC
  GGTAGAAGTCATGCGGAGCTTTTTAAACATGATTGGGGTGATGTTGGACAGAAAGCGTGATTTCGAGTTG
  GCCCAGGCATACCTTGCGTTGTTTTTGAAGTTACACCTCAGAACGCTTCCTTCGGAGCCAGTGCTTTTAG
  AGGAGCTGGTGAAGTTGTCATCACAGGTAGAAAAAGATTGGACTCACTTACAGTCCCTCTTCAATCAAAG
  CATGTGTGTCTTAAATTACATCAAAAGTGCTTTCTTG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG211095 representing BC094453
  Red=Cloning site Green=Tags(s)
  
  MAEMESAVEGRTASVLFAGFRALGLFSNEVPHVVRYSALKRRFYVTTCVGKSFHTYDVQKLSLVAVSNSV
  PQDICCMAADGRLVFAAYGNVFSAFARNKEIVHTFKGHKAEIHLLQPFGDHVISVDTDSVLIIWHIYSEE
  EYLQLTFDKSVFKISTILHPSTYLNKVLLGSEQGSLQLWNIKSNKLLYTFPGWKVGVTALQQAPAVDVVA
  IGLVSGQVVIHNIKYDETLMKFHQDWGPITSISFRTDGHPIMAAGSPCGHIGLWDLEDKKLINQMRNAHS
  TAISGLTFLHREPLLVTNGADNALRIWIFDGPAGEGRLLRFRMGHSASLTKIRYYGQNGQQILSASQDGT
  LQSFSTVHEKFNKSLGHGLVNKKIVKRKGLQNTMSVRLPPITQFAAEEARQSDWDGIIACHQGKRSCSTW
  NYQRSTIGAYFLKPRGVKTNSNLTATAVDITSCGNFAIIGLSSGAVDVYNMQSGIHRGNFGDDKAHTGSV
  RGVAVDGLNQLVVTAGSERLLKFWNFKSKVLIHSLGLDSSPNMMLLHRDSGILGLAMDDFSIAVLDIETR
  KIVREFSGHHGQINDMTFSPDGRWLISAAMDCSVRTWDLPSGCLIDCFLLDSAPLNVTMSPTGDFLATSH
  VDHLGIYLWSNISLYSVVSLRPLPPDYVPSIVMLPGTCQTQGMEDLEEKTEPSDEMIEYESPEQLSEQLV
  TLSLLPESRWKNLLNLDVIKKKNKPKEPPKVPQSAPFFIPTVPGLVPRFAVPEPSSDPQQSKVVNLGILA
  QKSNFYLKLEEGLLNNQYEAALNLLKELGPSGIETELRNLSPDDGGSVEVMRSFLNMIGVMLDRKRDFEL
  AQAYLALFLKLHLRTLPSEPVLLEELVKLSSQVEKDWTHLQSLFNQSMCVLNYIKSAFL
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC094453
• ORF Size: 2699 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC094453, AAH94453
• RefSeq Size: 2857 bp
• RefSeq ORF: 2699 bp
• Locus ID: 225348
• Cytogenetics: 18 B1