2310066E14Rik (BC030451) Mouse Tagged ORF Clone

CAT#: MG210992

2310066E14Rik (tGFP-tagged) - Mouse RIKEN cDNA 2310066E14 gene (cDNA clone MGC:40645 IMAGE:5400266)

Product Images

Circular map for MG210992

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: 2310066E14Rik
• Synonyms: 2310066E14Rik; AI837433
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG210992 representing BC030451
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTACGGCGACAGGAGGAGCTCGAGAATGGGACGGCCTGGTCCCTGTCCTCTGAATCGTCAGATGATT
  CATCAAGCCCGCAGCTCTCTGGCACAGCTCGACACTCAACTCCCAAGCCTCTGGTGCAACAGCCTGAGCC
  CCTGCCTGTCCAAGTTGCCTTCCGAAGGCCAGAGAGCCTCACGTCTGGTTCCATGGATGAAGAGCCAGCC
  ATGACCCCATCCCTGGTCAATGGGCATGCCCCTTACAGCCGGACTCTCAGCCACATCAGCGAGGCCAGCG
  TGGATGCTGCCTTGACTGAGGCCGTGGAAGCTGTGGACTCACAAAGCCCAATCCCAGGGCCTAGCCCGCT
  TGTCTATCCAGATTCCACCCACGTGGAGCGTGTCAGTAGTGTTCTGCCTGTCCTCAACAATGGCCATTCT
  GCCACAAGTCCCGCCCTCAGTACAACTGGCCCCGCCCCCACATTTATAGACCCTGCCCCAACGACACAGC
  TAGACTTGGTTCACAAAACCACAGACTCCGCCCCTTCTGAGTTGCCCAGCATCACACATACTACGACAAG
  CTCTGCATATAGTGCTGTAAGCCTTGTCAACAGTGTTCCAAGCCTAACTTCCACCACTATAGGTTCTGCC
  CACACAACCACGCCCTCTCCCCTCACCTCCACAGGCTCTGTTCCCAATGCAACAGACTCAACCCAGGCTA
  CCCCAAGCCCTACTCACAGTACCCCAAGCCCTACCCATACTACTATACGCCTCACCCACACCACTGTAAG
  CCCTACACATAGCTCCCCGAGCCCCATACACACTACCCCGAGCCCCACCCATACCACTGTAAGTCCCACC
  TGCACTACTCCAAGCTCCGGCCACAGTACCACTAGTCCCACTCAAGAAGCCAAGATGTCAACTCATACCA
  CCGGTGCTGTGGGCCCGGTCCAGACCACTACAAGTCCCATTTCCACAACTGAAAGCCCCTCCCCTTCTAC
  AGACGTGGCTATAATCTCCAGCTCCTCTGCAGAATCCACCGGCCCAGGCACTGAACCTCTGCCCTGTAGT
  CACCCAGCCTCCCCTCCCTACACTAAAGCAGACCCCACAGCTTCCTGCACCTCTTACCAGAGTCTTGCTT
  CCTCTGGTTCAAAACCCCTCACAAGCCCTGCCCCGGATTCCCCAGAACAAATCCCTAAGAGCCCAAGTTC
  CTCTCCCTCATCTAGTGCCCCTGAACCCCAACATTCAGAACATAACCTGGCCGCAGTTGCTCAAGCCCCA
  GTCCCTGAGGCAACGGGAGGAGCTGGGGACAGAAGGCTTGAAGAAGCTCTTGGGACCCTAATGTCTGCCC
  TTGATGACTATCGTGGTCAGTTCCCTGAGCTGCAGGGCCTGGAGCAGGAGGTGACTCGGCTGGAGAGTCT
  GCTCATGCAGAGACAAGGCCTGACTCGAAGCCGGGCTTCCAGTCTTAGCATCACTGTGGAGCATGCCCTG
  GAGAGCTTCAGCTTCCTCAATGATGATGAAGATGAAGACAACGATGGTCCCGGGGACAGGCACACAAGCA
  GCCCAGAGGTTGTGGCAGAGGACAGACTAGACTCATCAAATGGCCAGTCTCTAAGCACAGGGTGTTCAGC
  CCTGGATGCTACCTTGGTCCAGCACCTGTACCACTGCAGCCGCCTCCTGCTGAAACTGGGCACATTTGGA
  CCCCTGCGCTGCCAGGAGGCCTGGGCCCTGGAACGGCTGCTGCGGGAAGCGCGTGTATTCCAGGAAGTGT
  GTGAACGCAGCAAGCTATGGGGGAACTCTGCCACATCTGCCCAGGAAGTGGTACAGTTCTCCGCCTCTCG
  GCCCGGCTTCCTGACCTTTTGGGACCAGTGTACAGAGGGACTCAGCCCCTTCATCTGCTCTGTGGAGCGG
  GTGCTCCTCACCTTCTGCAGCCAGTACGGTGCCCGTCTTTCCCTGCGCCAGCCAGGCTTAGCCGAGGCTG
  TGTGTGTGAAGTTCCTGGAAGATGCCCTGGGACAGAAGCTGCCCAGGAGGCCCCAACCAGGCCCGGGGGA
  GCAGTTCACCATCTTCCAATTCTGGAGTTATGTAGAAGCCTTGGACAGCCCCTCCATGGATGCCTGTGTG
  ACAGAGACTGCAGAGGAGGTGTTACTAGTACAGAACCTGAACTCTGATGACCAGGCCGTTGTGCTGAAAG
  CCCTAAGGTTAGCACCGGAGGGGCGCCTGCGAAAGGATGGGCTTCGGGCTCTTAGCTCCCTGCTGGTCCA
  CGGCAACAACAAAGTCATGGCTGCTGTCAGCACTCAGCTTCGGAGCCTGTCACTTGGTCCTGTCTTCCGG
  GAAAGGGCTCTCTTGTGCTTCCTGGACCAGCTGGAAGATGGGGACGTGCAGACTCGAGTGGCCGGATGCC
  TGGCTTTGGGCTGTATCAAGGCTCCTGAGGGCATTGAGCCCCTGGTGTACTTGTGCCAAACAGACACAGA
  AGCTGTGAGGGAAGCCGCCCGGCAGAGCCTGCAGCAGTGCGGGGAGGAAGGACAGTCTGCTCATCGACAG
  CTAGAGGAGTCCCTGGATGCTCTGCCCTGCATCTTTGGGCCCAGCAGCATGGCCAGCACAGCATTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG210992 representing BC030451
  Red=Cloning site Green=Tags(s)
  
  MLRRQEELENGTAWSLSSESSDDSSSPQLSGTARHSTPKPLVQQPEPLPVQVAFRRPESLTSGSMDEEPA
  MTPSLVNGHAPYSRTLSHISEASVDAALTEAVEAVDSQSPIPGPSPLVYPDSTHVERVSSVLPVLNNGHS
  ATSPALSTTGPAPTFIDPAPTTQLDLVHKTTDSAPSELPSITHTTTSSAYSAVSLVNSVPSLTSTTIGSA
  HTTTPSPLTSTGSVPNATDSTQATPSPTHSTPSPTHTTIRLTHTTVSPTHSSPSPIHTTPSPTHTTVSPT
  CTTPSSGHSTTSPTQEAKMSTHTTGAVGPVQTTTSPISTTESPSPSTDVAIISSSSAESTGPGTEPLPCS
  HPASPPYTKADPTASCTSYQSLASSGSKPLTSPAPDSPEQIPKSPSSSPSSSAPEPQHSEHNLAAVAQAP
  VPEATGGAGDRRLEEALGTLMSALDDYRGQFPELQGLEQEVTRLESLLMQRQGLTRSRASSLSITVEHAL
  ESFSFLNDDEDEDNDGPGDRHTSSPEVVAEDRLDSSNGQSLSTGCSALDATLVQHLYHCSRLLLKLGTFG
  PLRCQEAWALERLLREARVFQEVCERSKLWGNSATSAQEVVQFSASRPGFLTFWDQCTEGLSPFICSVER
  VLLTFCSQYGARLSLRQPGLAEAVCVKFLEDALGQKLPRRPQPGPGEQFTIFQFWSYVEALDSPSMDACV
  TETAEEVLLVQNLNSDDQAVVLKALRLAPEGRLRKDGLRALSSLLVHGNNKVMAAVSTQLRSLSLGPVFR
  ERALLCFLDQLEDGDVQTRVAGCLALGCIKAPEGIEPLVYLCQTDTEAVREAARQSLQQCGEEGQSAHRQ
  LEESLDALPCIFGPSSMASTAF
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC030451
• ORF Size: 2586 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC030451.1
• RefSeq Size: 3209 bp
• RefSeq ORF: 2588 bp
• Locus ID: 75687
• Cytogenetics: 8 D3
• Gene Summary: Downstream effector protein for Rho-type small GTPases that plays a role in cell polarity and directional migration. Acts as an adapter protein, linking active Rho proteins to STK24 and STK26 kinases, and hence positively regulates Golgi reorientation in polarized cell migration upon Rho activation. Involved in the subcellular relocation of STK26 from the Golgi to cytoplasm punctae in a Rho- and PDCD10-dependent manner upon serum stimulation.[UniProtKB/Swiss-Prot Function]