Dpp9 (NM_172624) Mouse Tagged ORF Clone

CAT#: MG210990

Dpp9 (tGFP-tagged) - Mouse dipeptidylpeptidase 9 (Dpp9)

Product Images

Circular map for MG210990

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Dpp9
• Synonyms: 6430584G11Rik; A330078I11; DPP IX; DPRP2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG210990 representing NM_172624
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGCAGCGGGGTCTCCCCAGTTGAGCAGGTGGCCGCAGGGGACATGGATGACACGGCAGCACGCTTCT
  GTGTGCAGAAGCACTCGTGGGATGGGCTGCGTAGCATTATCCACGGCAGTCGCAAGTCCTCGGGCCTCAT
  TGTCAGCAAGGCCCCCCACGACTTCCAGTTTGTGCAGAAGCCTGACGAGTCTGGCCCCCACTCTCACCGT
  CTCTATTACCTCGGAATGCCTTACGGCAGCCGTGAGAACTCCCTCCTCTACTCCGAGATCCCCAAGAAAG
  TGCGGAAGGAGGCCCTGCTGCTGCTGTCCTGGAAGCAGATGCTGGACCACTTCCAGGCCACACCCCACCA
  TGGTGTCTACTCCCGAGAGGAGGAGCTACTGCGGGAGCGCAAGCGCCTGGGCGTCTTCGGAATCACCTCT
  TATGACTTCCACAGTGAGAGCGGCCTCTTCCTCTTCCAGGCCAGCAATAGCCTGTTCCACTGCAGGGATG
  GTGGCAAGAATGGCTTTATGGTGTCCCCGATGAAGCCACTGGAGATCAAGACTCAGTGTTCTGGGCCACG
  CATGGACCCCAAAATCTGCCCCGCAGACCCTGCCTTCTTTTCCTTCATCAACAACAGTGATCTGTGGGTG
  GCAAACATCGAGACTGGGGAGGAACGGCGGCTCACCTTCTGTCACCAGGGTTCAGCTGGTGTCCTGGACA
  ATCCCAAATCAGCAGGCGTGGCCACCTTTGTCATCCAGGAGGAGTTCGACCGCTTCACTGGGTGCTGGTG
  GTGCCCCACGGCCTCTTGGGAAGGCTCCGAAGGTCTCAAGACGCTGCGCATCCTATATGAGGAAGTGGAC
  GAGTCTGAAGTGGAGGTCATTCATGTGCCCTCCCCCGCCCTGGAGGAGAGGAAGACGGACTCCTACCGCT
  ACCCCAGGACAGGCAGCAAGAACCCCAAGATTGCCCTGAAGCTGGCTGAGCTCCAGACGGACCATCAGGG
  CAAAATCGTGTCAAGCTGCGAGAAGGAACTGGTACAGCCATTCAGCTCCCTTTTCCCCAAAGTGGAGTAC
  ATCGCCCGGGCTGGCTGGACACGGGACGGCAAATATGCCTGGGCCATGTTCCTGGACCGTCCCCAGCAAC
  GGCTTCAGCTTGTCCTCCTGCCCCCTGCTCTCTTCATCCCGGCCGTTGAGAGTGAGGCCCAGCGGCAGGC
  AGCTGCCAGAGCCGTCCCCAAGAATGTGCAGCCCTTTGTCATCTATGAAGAAGTCACCAATGTCTGGATC
  AACGTCCACGACATCTTCCACCCGTTTCCTCAGGCTGAGGGCCAGCAGGACTTTTGTTTCCTTCGTGCCA
  ACGAATGCAAGACTGGCTTCTGCCACCTGTACAGGGTCACAGTGGAACTTAAAACCAAGGACTATGACTG
  GACGGAACCCCTCAGCCCTACAGAAGATGAGTTTAAGTGCCCCATCAAGGAGGAGGTCGCCCTGACCAGT
  GGCGAGTGGGAGGTCTTGTCGAGGCATGGCTCCAAGATCTGGGTCAACGAGCAGACGAAGCTGGTGTACT
  TTCAAGGTACAAAGGACACACCGCTGGAACATCACCTCTATGTGGTCAGCTACGAGTCAGCAGGCGAGAT
  CGTGCGGCTCACCACGCTCGGCTTCTCCCACAGCTGCTCCATGAGCCAGAGCTTCGACATGTTCGTGAGT
  CACTACAGCAGTGTGAGCACGCCACCCTGTGTACATGTGTACAAGCTGAGCGGCCCCGATGATGACCCAC
  TGCACAAGCAACCACGCTTCTGGGCCAGCATGATGGAGGCAGCCAATTGCCCCCCAGACTATGTGCCCCC
  TGAGATCTTCCACTTCCACACCCGTGCAGACGTGCAGCTCTACGGCATGATCTACAAGCCACACACCCTG
  CAACCTGGGAGGAAGCACCCCACTGTGCTCTTTGTCTATGGGGGCCCACAGGTGCAGTTGGTGAACAACT
  CCTTTAAGGGCATCAAATACCTGCGGCTAAATACACTGGCATCCTTGGGCTATGCTGTGGTGGTGATCGA
  TGGTCGGGGCTCCTGTCAGCGGGGCCTGCACTTCGAGGGGGCCCTGAAAAATCAAATGGGCCAGGTGGAG
  ATTGAGGACCAGGTGGAAGGCTTGCAGTACGTGGCTGAGAAGTATGGCTTCATTGACTTGAGCCGAGTCG
  CCATCCATGGCTGGTCCTACGGCGGCTTCCTCTCACTCATGGGGCTCATCCACAAGCCACAAGTGTTCAA
  GGTAGCCATTGCGGGCGCTCCTGTCACTGTGTGGATGGCCTATGACACAGGGTACACGGAACGATACATG
  GATGTCCCCGAAAATAACCAGCAAGGCTATGAGGCAGGGTCTGTAGCCCTGCATGTGGAGAAGCTGCCCA
  ATGAGCCTAACCGCCTGCTTATCCTCCACGGCTTCCTGGACGAGAACGTTCACTTCTTCCACACAAATTT
  CCTGGTGTCCCAGCTGATCCGAGCAGGAAAGCCATACCAGCTTCAGATCTACCCAAACGAGAGACATAGC
  ATCCGCTGCCGCGAGTCCGGAGAGCATTACGAGGTGACGCTGCTGCACTTTCTGCAGGAACACCTG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG210990 representing NM_172624
  Red=Cloning site Green=Tags(s)
  
  MCSGVSPVEQVAAGDMDDTAARFCVQKHSWDGLRSIIHGSRKSSGLIVSKAPHDFQFVQKPDESGPHSHR
  LYYLGMPYGSRENSLLYSEIPKKVRKEALLLLSWKQMLDHFQATPHHGVYSREEELLRERKRLGVFGITS
  YDFHSESGLFLFQASNSLFHCRDGGKNGFMVSPMKPLEIKTQCSGPRMDPKICPADPAFFSFINNSDLWV
  ANIETGEERRLTFCHQGSAGVLDNPKSAGVATFVIQEEFDRFTGCWWCPTASWEGSEGLKTLRILYEEVD
  ESEVEVIHVPSPALEERKTDSYRYPRTGSKNPKIALKLAELQTDHQGKIVSSCEKELVQPFSSLFPKVEY
  IARAGWTRDGKYAWAMFLDRPQQRLQLVLLPPALFIPAVESEAQRQAAARAVPKNVQPFVIYEEVTNVWI
  NVHDIFHPFPQAEGQQDFCFLRANECKTGFCHLYRVTVELKTKDYDWTEPLSPTEDEFKCPIKEEVALTS
  GEWEVLSRHGSKIWVNEQTKLVYFQGTKDTPLEHHLYVVSYESAGEIVRLTTLGFSHSCSMSQSFDMFVS
  HYSSVSTPPCVHVYKLSGPDDDPLHKQPRFWASMMEAANCPPDYVPPEIFHFHTRADVQLYGMIYKPHTL
  QPGRKHPTVLFVYGGPQVQLVNNSFKGIKYLRLNTLASLGYAVVVIDGRGSCQRGLHFEGALKNQMGQVE
  IEDQVEGLQYVAEKYGFIDLSRVAIHGWSYGGFLSLMGLIHKPQVFKVAIAGAPVTVWMAYDTGYTERYM
  DVPENNQQGYEAGSVALHVEKLPNEPNRLLILHGFLDENVHFFHTNFLVSQLIRAGKPYQLQIYPNERHS
  IRCRESGEHYEVTLLHFLQEHL
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_172624
• ORF Size: 2586 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_172624.3
• RefSeq Size: 3457 bp
• RefSeq ORF: 2589 bp
• Locus ID: 224897
• UniProt ID: Q8BVG4
• Cytogenetics: 17 D
• Gene Summary: Dipeptidyl peptidase that cleaves off N-terminal dipeptides from proteins having a Pro or Ala residue at position 2.[UniProtKB/Swiss-Prot Function]