Vps16 (NM_030559) Mouse Tagged ORF Clone

CAT#: MG210911

Vps16 (tGFP-tagged) - Mouse vacuolar protein sorting 16 (yeast) (Vps16)

Product Images

Circular map for MG210911

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Vps16
• Synonyms: 1810074M16Rik
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG210911 representing NM_030559
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACTGTTACACTGCGAACTGGAATCCACTCGGGGACTCTGCCTTTTACAGGAAGTATGAGCTGTACA
  GCATGGACTGGGACCTGAAGGAGGAGCTCAAGGATTGCCTGGTGGCTGCTGCACCCTATGGGGGTCCCAT
  TGCTCTACTGAGGAACTGTTGGAGAAAAGAGAAAGCTGCCAGCGTGCGGCCAGTACTGGAGATCTACTCT
  GCATCTGGCTTACCACTGGCTAGCCTGCTGTGGAAGAGTGGGCCTGTGGTGGCCCTCGGCTGGTCAGCTG
  AGGAGGAGCTGCTCTGTGTGCAAGAAGATGGTGCAGTGCTAGTTTATGGCCTTCATGGAGACTTCCGGAG
  GCACTTCAGCATGGGCAATGAAGTGCTTCAGAACCGTGTCCTGGATGCCCGGATTTTTCACACTGAGTTT
  GGTTCAGGGGTGGCCATCCTTACAGGAGCCTATCGCTTTACTCTGAGTGCCAATGTGGGTGACCTCAAAC
  TCCGTCGGATGCCGGAGGTGCCAGGTATGCAAAGTGCACCCTCATGTTGGACCACACTGTGCCACGACCG
  AGTACCACACATTCTTCTGGCTGTAGGACCCGATCTTTACCTTCTGGATCATGCTACCTGCTCTGCAGTG
  ACACCTGCTGGCTTAGCCCCAGGAGTGAGCAGCTTCCTACAGATGGCTGTATCCTTCACGTATCGTTACC
  TGGCACTCTTCACAGACACAGGCTACATCTGGATGGGGACAGCATCACTCAAGGAGAAGCTGTGTGAGTT
  CAACTGCAACATCCGGGCTCCCCCGAAGCAGATGGTCTGGTGTAGCCGTCCTCGGAGCAAGGAAAGGGCT
  GTTGTTGTGGCCTGGGAGAGGCGGCTGATGGTGGTGGGCAATGCGCCTGAAAGCATCCAGTTTGTGCTCG
  ATGAGGACTCCTACTTAGTGCCTGAGCTTGATGGGGTCCGCATCTTCTCCCGAAGCACCCATGAGTTCCT
  GCATGAAGTCCCAGTGGCCAGTGAAGAGATCTTCAAAATTGCCTCAATGGCCCCTGGAGCACTGCTGTTA
  GAGGCCCAGAAGGAATATGAGAAAGAGAGCCAGAAAGCAGATGAGTACCTGCGGGAGATCCAGGAACTGG
  GCCAGCTGATCCAGGCTGTGCAGCAGTGCATTGAGGCTGCAGGACATGAGCACCAGCCAGACATGCAAAA
  GAGTCTGCTCAGGGCGGCTTCATTTGGAAAGTGTTTCCTTGACAGATTTCCACCTGACAGCTTCGTGCAC
  ATGTGTCAGGACCTTCGAGTGCTCAATGCTATTAGAGACTACCACATTGGGATCCCTCTCACCTACACCC
  AATACAAGCAGCTCACCATCCAGGTGCTGCTAGACAGGCTTGTGTTGCGGAGGCTTTATCCCCTGGCTAT
  CCAGATATGCGAGTACCTGCGCCTTCCTGAAGTCCAGGGTGTCAGCAGGATCCTAGCCCACTGGGCCTGC
  TACAAGGTGCAGCAGAAAGATGTCTCAGATGAAGATGTAGCACGGGCTATTAATCAGAAGCTGGGTGACA
  CGCCTGGTGTTTCATACTCTGACATTGCTGCGAGGGCCTATGGCTGTGGCCGCACTGAACTGGCCATTAA
  GCTGCTGGAGTATGAGCCACGCTCAGGGGAGCAGGTACCTCTTCTCCTAAAGATGAAGAGGAGCAAACTG
  GCACTGAGCAAGGCTATTGAGAGTGGAGACACTGACCTGGTGTTTACAGTGTTGCTGCACCTAAAGAATG
  AACTCAACCGAGGAGACTTTTTCATGACTCTCCGGAACCAACCCATGGCCCTGAGTTTGTATCGACAGTT
  CTGTAAGCATCAGGAGCTAGACACTCTGAAGGACCTTTACAATCAGGACGACAATCACCAGGAGTTGGGC
  AGCTTCCACATCAGAGCCAGCTATGCCGCTGAAGAGCGTATTGAAGGGCGAGTGGCAGCTCTGCAGATGG
  CAGCTGATGCCTTCTACAAAGCCAAGAATGAATTTGCAGCCAAGGCCACAGAGGATCAAATGAGGCTCCT
  GCGGATACAGCGACGCCTAGAGGATGAGCTAGGGGGCAGGTTCCTAGACCTATCTCTACACGACACTGTC
  ACCACTCTTATCCTTGGAGGCCACAACAAGCGGGCAGAGCAGCTGGCACGTGACTTTCGCATCCCTGATA
  AGAGACTATGGTGGTTAAAGCTGGCTGCCCTGGCAGATTTGGAAGATTGGGAGGAGCTGGAGAAGTTTTC
  CAAAAGCAAGAAATCACCGATTGGTTACCTGCCCTTTGTAGAGATCTGCATGAAACAGCATAACAAACAT
  GAGGCCAAGAAGTATGCTTCCCGTGTGGGCCCTGAACAAAAGGTCAAGGCTTTGCTTCTTGTTGGGGATG
  TGGCTCAGGCTGCAGAGGTGGCTATTGAGCACCGGAATGAAACAGAGCTGAGCCTCGTTTTGTCCCACTG
  CACTGGAGCCACAGATGGGGCCATAGCTGACAAGATTCAGCGGGCCAGGGCACAAGCCCAGAAGAAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG210911 representing NM_030559
  Red=Cloning site Green=Tags(s)
  
  MDCYTANWNPLGDSAFYRKYELYSMDWDLKEELKDCLVAAAPYGGPIALLRNCWRKEKAASVRPVLEIYS
  ASGLPLASLLWKSGPVVALGWSAEEELLCVQEDGAVLVYGLHGDFRRHFSMGNEVLQNRVLDARIFHTEF
  GSGVAILTGAYRFTLSANVGDLKLRRMPEVPGMQSAPSCWTTLCHDRVPHILLAVGPDLYLLDHATCSAV
  TPAGLAPGVSSFLQMAVSFTYRYLALFTDTGYIWMGTASLKEKLCEFNCNIRAPPKQMVWCSRPRSKERA
  VVVAWERRLMVVGNAPESIQFVLDEDSYLVPELDGVRIFSRSTHEFLHEVPVASEEIFKIASMAPGALLL
  EAQKEYEKESQKADEYLREIQELGQLIQAVQQCIEAAGHEHQPDMQKSLLRAASFGKCFLDRFPPDSFVH
  MCQDLRVLNAIRDYHIGIPLTYTQYKQLTIQVLLDRLVLRRLYPLAIQICEYLRLPEVQGVSRILAHWAC
  YKVQQKDVSDEDVARAINQKLGDTPGVSYSDIAARAYGCGRTELAIKLLEYEPRSGEQVPLLLKMKRSKL
  ALSKAIESGDTDLVFTVLLHLKNELNRGDFFMTLRNQPMALSLYRQFCKHQELDTLKDLYNQDDNHQELG
  SFHIRASYAAEERIEGRVAALQMAADAFYKAKNEFAAKATEDQMRLLRIQRRLEDELGGRFLDLSLHDTV
  TTLILGGHNKRAEQLARDFRIPDKRLWWLKLAALADLEDWEELEKFSKSKKSPIGYLPFVEICMKQHNKH
  EAKKYASRVGPEQKVKALLLVGDVAQAAEVAIEHRNETELSLVLSHCTGATDGAIADKIQRARAQAQKK
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_030559
• ORF Size: 2517 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_030559.2, NP_085036.2
• RefSeq Size: 2617 bp
• RefSeq ORF: 2520 bp
• Locus ID: 80743
• Cytogenetics: 2 F1