Vav1 (BC020487) Mouse Tagged ORF Clone

CAT#: MG210763

Vav1 (tGFP-tagged) - Mouse vav 1 oncogene (cDNA clone MGC:11710 IMAGE:3965451)

Product Images

Circular map for MG210763

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Vav1
• Synonyms: Vav; vav-T
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG210763 representing BC020487
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCTCTGGCGACAGTGCACCCACTGGCTGATCCAGTGTCGGGTGCTGCCTCCCAGCCACCGTGTGA
  CCTGGGAGGGGGCCCAGGTGTGTGAGCTGGCACAGGCACTGCGGGACGGTGTCCTCTTGTGCCAATTGCT
  TAACAACCTGCTTCCCCAGGCCATTAATCTTCGCGAGGTTAACTTGCGGCCCCAGATGTCCCAGTTCCTT
  TGTCTTAAGAACATTCGAACCTTCCTGTCTACTTGCTGTGAGAAGTTCGGCCTCAAGCGCAGTGAACTCT
  TTGAGGCTTTTGACCTCTTCGATGTGCAGGACTTTGGAAAGGTCATCTACACCCTGTCTGCTCTGTCATG
  GACACCCATTGCCCAGAACAAAGGAATCATGCCCTTCCCAACAGAGGACAGCGCTCTGAACGACGAAGAT
  ATTTACAGTGGCCTTTCAGACCAGATTGATGACACCGCAGAGGAAGACGAGGACCTTTATGACTGCGTGG
  AAAATGAGGAGGCAGAGGGGGACGAGATCTACGAGGACCTAATGCGCTTGGAGTCGGTGCCTACGCCACC
  CAAGATGACAGAGTATGATAAGCGCTGCTGCTGCCTGCGGGAGATCCAGCAGACGGAGGAGAAGTATACA
  GACACACTGGGCTCCATCCAGCAGCACTTCATGAAGCCTCTGCAGCGATTCCTTAAGCCTCAAGACATGG
  AGACCATCTTTGTCAACATTGAGGAGCTGTTCTCTGTGCATACCCACTTCTTAAAGGAACTGAAGGATGC
  CCTGGCTGGCCCGGGAGCAACAACACTGTATCAGGTCTTCATCAAGTACAAGGAGAGGTTCCTGGTTTAT
  GGCCGTTATTGCAGTCAGGTGGAGTCAGCCAGCAAGCACTTGGATCAAGTGGCCACAGCACGGGAGGATG
  TGCAGATGAAGCTGGAGGAATGTTCTCAAAGAGCTAACAATGGCCGATTCACCCTACGGGATCTGCTGAT
  GGTACCTATGCAGCGGGTGCTGAAGTACCACCTCCTTCTCCAGGAGCTAGTGAAACACACACAGGATGCT
  ACAGAGAAGGAGAACCTGCGGTTGGCCCTGGACGCCATGAGGGACCTGGCACAGTGCGTGAACGAGGTCA
  AGAGGGACAATGAAACCCTACGGCAGATCACAAACTTTCAGCTGTCCATTGAGAACCTGGACCAGTCTCT
  GGCTAACTATGGCCGGCCCAAGATTGACGGTGAGCTCAAGATTACCTCAGTGGAGCGTCGCTCAAAGACA
  GACAGGTATGCCTTCCTGCTGGACAAAGCACTGCTCATCTGTAAACGCCGCGGGGACTCTTACGACCTCA
  AAGCCTCGGTGAACTTGCACAGCTTCCAAGTTCGAGATGACTCCTCCGGGGAGCGAGACAACAAGAAGTG
  GAGCCATATGTTCCTTCTGATTGAGGATCAAGGCGCCCAGGGCTATGAGCTGTTCTTCAAGACTCGGGAG
  CTGAAGAAGAAGTGGATGGAACAGTTCGAAATGGCCATCTCCAACATTTACCCAGAGAATGCTACAGCCA
  ATGGGCATGATTTTCAGATGTTCTCCTTTGAGGAGACCACTTCCTGCAAGGCCTGCCAGATGTTACTCAG
  AGGCACATTCTACCAGGGATATCGCTGTTACAGGTGCCGGGCACCTGCACACAAGGAGTGTCTGGGGAGA
  GTGCCTCCCTGTGGTCGCCATGGGCAAGATTTCGCAGGAACCATGAAGAAGGACAAGCTCCATCGAAGGG
  CCCAGGACAAGAAAAGGAATGAATTGGGTCTGCCTAAGATGGAAGTGTTTCAGGAATACTATGGGATCCC
  TCCTCCACCTGGAGCCTTTGGGCCATTTTTACGGCTCAACCCTGGGGACATTGTGGAGCTCACTAAGGCA
  GAGGCTGAGCACAACTGGTGGGAGGGAAGGAATACTGCTACAAATGAAGTCGGCTGGTTTCCCTGTAACA
  GAGTGCATCCCTATGTCCACGGCCCTCCTCAGGACCTGTCTGTGCATCTCTGGTATAACGTGGAGGTCAA
  GCATATTAAAATCATGACGTCAGAGGGGTTGTACCGGATCACAGAGAAGAAGGCTTTCCGGGGCCTTCTG
  GAACTGGTAGAGTTTTATCAGCAGAATTCCCTCAAAGATTGCTTCAAGTCGTTGGACACCACCTTGCAGT
  TTCCTTATAAGGAACCTGAGAGGAGAGCCATCAGCAAGCCACCAGCTGGAAGCACCAAGTATTTTGGCAC
  TGCCAAAGCCCGCTACGACTTCTGTGCCCGGGACAGGTCGGAACTGTCCCTTAAGGAGGGTGATATCATC
  AAGATCCTCAATAAGAAGGGACAGCAAGGCTGGTGGCGTGGGGAGATCTACGGCCGGATCGGCTGGTTCC
  CTTCTAACTATGTGGAGGAAGACTATTCCGAATATTGC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG210763 representing BC020487
  Red=Cloning site Green=Tags(s)
  
  MELWRQCTHWLIQCRVLPPSHRVTWEGAQVCELAQALRDGVLLCQLLNNLLPQAINLREVNLRPQMSQFL
  CLKNIRTFLSTCCEKFGLKRSELFEAFDLFDVQDFGKVIYTLSALSWTPIAQNKGIMPFPTEDSALNDED
  IYSGLSDQIDDTAEEDEDLYDCVENEEAEGDEIYEDLMRLESVPTPPKMTEYDKRCCCLREIQQTEEKYT
  DTLGSIQQHFMKPLQRFLKPQDMETIFVNIEELFSVHTHFLKELKDALAGPGATTLYQVFIKYKERFLVY
  GRYCSQVESASKHLDQVATAREDVQMKLEECSQRANNGRFTLRDLLMVPMQRVLKYHLLLQELVKHTQDA
  TEKENLRLALDAMRDLAQCVNEVKRDNETLRQITNFQLSIENLDQSLANYGRPKIDGELKITSVERRSKT
  DRYAFLLDKALLICKRRGDSYDLKASVNLHSFQVRDDSSGERDNKKWSHMFLLIEDQGAQGYELFFKTRE
  LKKKWMEQFEMAISNIYPENATANGHDFQMFSFEETTSCKACQMLLRGTFYQGYRCYRCRAPAHKECLGR
  VPPCGRHGQDFAGTMKKDKLHRRAQDKKRNELGLPKMEVFQEYYGIPPPPGAFGPFLRLNPGDIVELTKA
  EAEHNWWEGRNTATNEVGWFPCNRVHPYVHGPPQDLSVHLWYNVEVKHIKIMTSEGLYRITEKKAFRGLL
  ELVEFYQQNSLKDCFKSLDTTLQFPYKEPERRAISKPPAGSTKYFGTAKARYDFCARDRSELSLKEGDII
  KILNKKGQQGWWRGEIYGRIGWFPSNYVEEDYSEYC
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC020487
• ORF Size: 2420 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC020487, AAH20487
• RefSeq Size: 2773 bp
• RefSeq ORF: 2420 bp
• Locus ID: 22324
• Cytogenetics: 17 29.76 cM
• Gene Summary: Couples tyrosine kinase signals with the activation of the Rho/Rac GTPases, thus leading to cell differentiation and/or proliferation.[UniProtKB/Swiss-Prot Function]