Pcdhb17 (NM_053142) Mouse Tagged ORF Clone

CAT#: MG210722

Pcdhb17 (tGFP-tagged) - Mouse protocadherin beta 17 (Pcdhb17)

Product Images

Circular map for MG210722

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Pcdhb17
• Synonyms: mKIAA1621; Pcdhb16; PcdhbQ
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG210722 representing NM_053142
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGACTGCATGGATGTGCAATCTGAGACAAAGGCAAGTCCTGGCTTTCTTTGTTTTGCTGCATGTGT
  CAGGGGCAGGGGCAGAACTGGGCCCCTATTCCATAGAGGAAGAAACAGAGAGGGGCTCCTTTGTGGCAAA
  TCTTGGGAAAGATCTGGGGGTGGACCTGGCAGAGATATCCAATCGCAGGGCTCGGATCATTTCCCAAGAA
  AACAAAGAGCATTTGCAGCTCAACCTTCAATCTGGAGATCTGCTCATAAATGAGAAACTGGACCGAGAGG
  AGCTATGTGGCCCCATTGAGCCGTGTGTTCTACACTTCCAAGTGTTAATGGAAAACCCTTTAGAGGTATT
  TCAGGCTGAACTGAGGGTGATGGACATAAATGACTATTCTCCGGTGTTCAGTGAAAGAGAAATGATACTG
  AGAATACTAGAAAACAGTGCTCTGGGAGATACATTTCCTTTAAATAATGCTCTGGACTCAGACGTAGCAA
  TCAACAATATCCAGACCTATAGGCTCAGCTCAAACTCTCATTTCCTGGTTGTAACCCGCAACCGCAGTGA
  TGGCAGGAAGTACCCAGAGCTGGTGCTGGAGAAAGAACTGGATCGAGAGGAGGAACCTGAGCTGAGGTTA
  ACTCTGACAGCTTTGGATGGTGGCGCTCCTCCCCGGTCTGGGACGGCACAGGTTCTCATTGAAGTAGTGG
  ACATCAACGATAATGCACCCAAGTTTCAGCAGCCAACATACCGAGTGCAAATTCCCGAGAACAGTCCCAC
  CGGTTCTCTGGTACTCACAGTCTCAGCCAATGACTTGGACAGTGGAGATTATGGAAAAGTATTGTATGCA
  CTTTCTCAGCCTTCAGAAGATATTAGCAAAACGTTAGAAGTAAACCCTGTAACCGGGGAGATTCGGCTAC
  GAAAAGAGGTGGATTTTGAAACTATTCCCTCTTATGAAGTGGATATCAAGGCCACGGATGGGGGAGGTCT
  CTCAGGAAAATGCACGCTGTTACTGAAGGTGGTGGACGTGAATGACAATGCCCCAGAAGTGATGCTATCT
  GCTCTTACCAGCCCAGTCCCAGAAAACTCCCCGGACGAGGTAGTTGCTGTTTTCAGTGTTAAAGATCCTG
  ATTCAGCGAACAATGGAAAAATGATTGCCTCCATCGAAGAAGATCTTCCCTTTCTTCTAAAATCTTCAGG
  GAAGAACTTTTACACTTTAGTGACCAAGAGAGCACTTGACAGGGAAGAAAGAGAGAAATACAACATCACC
  ATCACTGTCTCTGACCTGGGCACACCCAGGCTCACAACCCAGCACACCATAACAGTGCAGGTCTCTGACA
  CCAATGACAACGCCCCCGCCTTCAACCAAACCTCATACACCCTGTTCGTCCGCGAGAACAACAGCCCCGC
  CATGCACATAGGCACCATCAGTGCCACAGACTCTGACGCTGGCTCCAATTCCCACATCAGCTACTCGCTG
  TTGCCTTCCCACGACCCGCAGCTGGCCCTGGACTCGCTCATCTCCATCAACGCAGACAATGGGCAGCTGT
  TCGCGCTCAGGGCGCTGGACTATGAGGCCCTGCAGGCCTTTGAGTTCCACGTGGGTGCAATAGACCAAGG
  CTCACCTGCGCTCAGCAGCCAAGCGCTGGTGCGCGTGGTGGTGCTGGACGACAATGACAATGCGCCCTTC
  GTGCTCTACCCGATGCAGAACTCCTCTGCGCCTTGCACAGAACTGCTGCCCAGGGCGGCGGAACCCGGAT
  ACCTGGTCACCAAGGTGGTGGCTGTGGATCGCGACTCTGGCCAGAATGCCTGGCTGTCATTCCAACTTCT
  CAAGGCTACGGAGCCCGGGCTGTTCAGCGTGTGGGCGCACAATGGCGAGGTACGCACTACCAGACTGCTG
  AGCGAGCGCGATGTGCCCAAGCACAGGCTGGTCCTGCTGGTCAAGGACAATGGCGATCCTCCGCGCTCTG
  CCAGCGTCACGCTGCACGTGCTAGTGGTGGATGGCTTCTCTCAGCCCTACCTGCCTCTGCCAGAGGTGGC
  GCGCAATCCCGCGCACGATGAGGACGCTCTAACACTGTATCTGGTCATAGCCTTGGCATCTGTGTCTTCT
  CTCTTCCTCTTGTCTGTGCTGCTGTTTGTGGGGGTGAGGCTGTGCAGGAGGGCCAGGTCGGCCTCTCTGA
  GTGGTTATTCCGTGCCTGAGGGCCACATTCCTGGCCATCTGGTGGATGTCAGAGGTGTGGGGACCCTGTC
  CCAGAGCTACCAGTATGATGTATGTCTGATGGGCGATTCTTCTGGGACCAGTGAGTTTAACTTCTTAAAG
  CCAGTTTTCCCTAGCTCTCTAGACCAGTGCTCTGGTAAAGAAATAGAGGAAAATTCCTCACTCCACAATA
  GTTTTGGATTTCATCAT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG210722 representing NM_053142
  Red=Cloning site Green=Tags(s)
  
  METAWMCNLRQRQVLAFFVLLHVSGAGAELGPYSIEEETERGSFVANLGKDLGVDLAEISNRRARIISQE
  NKEHLQLNLQSGDLLINEKLDREELCGPIEPCVLHFQVLMENPLEVFQAELRVMDINDYSPVFSEREMIL
  RILENSALGDTFPLNNALDSDVAINNIQTYRLSSNSHFLVVTRNRSDGRKYPELVLEKELDREEEPELRL
  TLTALDGGAPPRSGTAQVLIEVVDINDNAPKFQQPTYRVQIPENSPTGSLVLTVSANDLDSGDYGKVLYA
  LSQPSEDISKTLEVNPVTGEIRLRKEVDFETIPSYEVDIKATDGGGLSGKCTLLLKVVDVNDNAPEVMLS
  ALTSPVPENSPDEVVAVFSVKDPDSANNGKMIASIEEDLPFLLKSSGKNFYTLVTKRALDREEREKYNIT
  ITVSDLGTPRLTTQHTITVQVSDTNDNAPAFNQTSYTLFVRENNSPAMHIGTISATDSDAGSNSHISYSL
  LPSHDPQLALDSLISINADNGQLFALRALDYEALQAFEFHVGAIDQGSPALSSQALVRVVVLDDNDNAPF
  VLYPMQNSSAPCTELLPRAAEPGYLVTKVVAVDRDSGQNAWLSFQLLKATEPGLFSVWAHNGEVRTTRLL
  SERDVPKHRLVLLVKDNGDPPRSASVTLHVLVVDGFSQPYLPLPEVARNPAHDEDALTLYLVIALASVSS
  LFLLSVLLFVGVRLCRRARSASLSGYSVPEGHIPGHLVDVRGVGTLSQSYQYDVCLMGDSSGTSEFNFLK
  PVFPSSLDQCSGKEIEENSSLHNSFGFHH
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_053142
• ORF Size: 2397 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_053142.3, NP_444372.2
• RefSeq Size: 3284 bp
• RefSeq ORF: 2400 bp
• Locus ID: 93888
• Cytogenetics: 18 B3