Rgl2 (NM_009059) Mouse Tagged ORF Clone

CAT#: MG210600

Rgl2 (tGFP-tagged) - Mouse ral guanine nucleotide dissociation stimulator-like 2 (Rgl2)

Product Images

Circular map for MG210600

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Rgl2
• Synonyms: KE1.5; Rab2l; Rgt2; Rlf
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG210600 representing NM_009059
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTCCCGCGGCCCCTGCGGCTGCTTTTGGACACGACCCCCCCCGGGGGAGTCGTGCTGAGCAGCTTCC
  GGAGCCGGGACCCCGAAGAGGGTGGGGACCCAGGTGGCCGGGCCGTGGGCGGGGGGCAGGAGGAAGAGGA
  TGAGGAAGAAGAAGAGGCTTCTGTGTCAGTCTGGGACGAGGAGGAGGATGGTGCGACCTTTACTGTCACA
  AGCCGCCAGTACAGGCCTCTTGACCCCTTGGCTCCCTTGCCTCCACCTCGCTCCTCCCGACGGCTCCGCG
  CTGGCACTCTGGAGGCCCTGGTCAGACACCTCTTGGATGCCAGGACAGCAGGGGCTGACATGATGTTCAC
  TCCGGCCTTGCTGGCCACCCACCGGGCCTTCACCTCCACTCCTGCCCTGTTTGGGCTTGTGGCTGACAGG
  CTGGAAGCCCTTGAATCTCATCCTCCCGGTGAGCTAGAGAGGACCACAGGGGTAGCCATCTCTGTACTTT
  CAACTTGGCTGGCCTCTCACCCTGAGGATTTTGGCTCTGAGGTCAAGGGTCAACTTGACCGGCTTGAGAG
  CTTCTTGCTTCGGACAGGGTATGCAGCACGGGAGGGTGTTGTGGGGGGCAGTGCTGACCTCATCCGAAAC
  CTCCGGGCCCGGGTGGACCCCCGGGCCCCCGACCTTCCTAAGCCCCTGGCCCTTCCTGGCGATTCCCCTG
  CTGACCCCACGGATGTCCTGGTGTTCCTCGCTGACCACTTGGCCGAACAGCTGACCCTGCTAGATGCGGA
  ACTGTTTCTTAATCTGATCCCCTCTCAGTGTTTGGGAGGCCTCTGGGGTCACAGAGACCGGCCAGGACAT
  TCTCACCTCTGCCCGTCTGTCCGAGCTACCGTCACACAGTTCAACAAGGTGGCGGGGGCGGTAGTTAGCT
  CTGTCTTGGGGGCCACCTCAATTGGAGAGGGGCCAAGAGAGGTGACTGTGAGACCACTGCGGCCCCCACA
  GAGGGCCCGGCTCCTAGAGAAGTGGATCCGTGTGGCCGAGGAGTGCCGCCTGCTTCGGAACTTCTCCTCA
  GTGTATGCTGTTGTGTCCGCTCTGCAGTCCAGCCCTATCCACAGGCTTCGGGCAGCCTGGGGGGAGACAA
  CCAGGGACAGCCTCCGAGTCTTTTCCAGCCTGTGCCAGATTTTCTCAGAGGAGGATAATTATTCCCAGAG
  CAGAGAGCTCCTCATGCAGGAAGTGAAGCCGCAGCCCCCTGTGGAGCCACACTCCAAGAAGGCCCCAAGG
  TCTGGCTTCAGGGGTGGGGGTGTGGTTCCCTACCTGGGAACCTTCCTGAAGGACCTCGTGATGCTGGATG
  CGGCCTCCAAGGATGAGCTGGAGAATGGCTACATCAATTTTGACAAGCGGAGGAAGGAGTTTGCTATCCT
  TTCGGAGCTGTTGCGCCTCCAGAAAGAATGTCGTGGCTACGACCTCCGACCTAACTCTGATATCCAGCAA
  TGGCTCCAGGGCCTCCAGCCTTTAACTGAAGCTCAGAGTCACCGTGTATCCTGTGAAGTGGAGCCACCAG
  GGACCAGTGACTCCCCCGCTGCAAGGACACCTCGGCCAACACTAGTGATCACACAGTGGACGGAAGTTCT
  GGGCTCTGTTGGAGGCCCCACTCCGCTTGTGTCCTGGGATCGGCCCAGTGTCGGGGGAGATGAGGTGCCT
  GGAACCCCAGCACCTCTGCTGACTCGCCTCGCCCAGCACATGAAGTGGCCATCAGTCTCATCTCTGGACT
  CTGCCCTGGAAAGCAGCCCCTCCTTGCACAGCCCTGCTGACCCTGGCCACCTCTCTCCTCCAGCCTCCTC
  CCCTAGGCCTTCCCGGGGTCACCGTCGCTCAGCCTCCTGTGGGTCTCCGTTGAGTGGAAACACAGGAGAA
  GGGACCTCTAGGAGTGCTGGATGTGGGGGCGGGGTATCTGGGCCAGGGTCCTCTGATTGCCGAATCATCC
  GAGTCCAGATGGAGCTGGGGGAGGATGGCAGCGTCTACAAGAGCATCCTGGTGACAAGCCAGGACAAAGC
  TCCAAGTGTTATTAGTCGTGTCCTTAAGAAAAACAATCGTGATTCTGCTGTGGCTTCAGAGTTCGAGCTG
  GTGCAGCTGCTACCTGGGGATCGAGAGCTGACCATCCCACACTCAGCTAACGTCTTCTATGCCATGGATG
  GTGCATCTCATGACTTCCTCCTGCGGCAGCGCAGAAGACCCTCTGCTGCCACCCCGGGTTCCCACAGCGG
  CCCCTCTGCCTCAGGAACTCCTCCGAGCGAGGGGGGAGGGGGCTCCTTTCCCAGGATCAAGGCCACGGGG
  AGGAAGATTGCACGGGCACTGTTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG210600 representing NM_009059
  Red=Cloning site Green=Tags(s)
  
  MLPRPLRLLLDTTPPGGVVLSSFRSRDPEEGGDPGGRAVGGGQEEEDEEEEEASVSVWDEEEDGATFTVT
  SRQYRPLDPLAPLPPPRSSRRLRAGTLEALVRHLLDARTAGADMMFTPALLATHRAFTSTPALFGLVADR
  LEALESHPPGELERTTGVAISVLSTWLASHPEDFGSEVKGQLDRLESFLLRTGYAAREGVVGGSADLIRN
  LRARVDPRAPDLPKPLALPGDSPADPTDVLVFLADHLAEQLTLLDAELFLNLIPSQCLGGLWGHRDRPGH
  SHLCPSVRATVTQFNKVAGAVVSSVLGATSIGEGPREVTVRPLRPPQRARLLEKWIRVAEECRLLRNFSS
  VYAVVSALQSSPIHRLRAAWGETTRDSLRVFSSLCQIFSEEDNYSQSRELLMQEVKPQPPVEPHSKKAPR
  SGFRGGGVVPYLGTFLKDLVMLDAASKDELENGYINFDKRRKEFAILSELLRLQKECRGYDLRPNSDIQQ
  WLQGLQPLTEAQSHRVSCEVEPPGTSDSPAARTPRPTLVITQWTEVLGSVGGPTPLVSWDRPSVGGDEVP
  GTPAPLLTRLAQHMKWPSVSSLDSALESSPSLHSPADPGHLSPPASSPRPSRGHRRSASCGSPLSGNTGE
  GTSRSAGCGGGVSGPGSSDCRIIRVQMELGEDGSVYKSILVTSQDKAPSVISRVLKKNNRDSAVASEFEL
  VQLLPGDRELTIPHSANVFYAMDGASHDFLLRQRRRPSAATPGSHSGPSASGTPPSEGGGGSFPRIKATG
  RKIARALF
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_009059
• ORF Size: 2334 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_009059.2, NP_033085.2
• RefSeq Size: 2967 bp
• RefSeq ORF: 2337 bp
• Locus ID: 19732
• UniProt ID: Q61193
• Cytogenetics: 17 B1
• Gene Summary: Probable guanine nucleotide exchange factor. Putative effector of Ras and/or Rap. Associates with the GTP-bound form of Rap 1A and H-Ras in vitro.[UniProtKB/Swiss-Prot Function]