Anapc5 (NM_021505) Mouse Tagged ORF Clone

CAT#: MG210396

Anapc5 (tGFP-tagged) - Mouse anaphase-promoting complex subunit 5 (Anapc5), transcript variant 1

Product Images

Circular map for MG210396

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Anapc5
• Synonyms: 2510006G12Rik; AA408751; AA536819; AA986414; Anpc5; APC5
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG210396 representing NM_021505
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATGACCAACGGGGTAGTGCACGCCAACTTGTTTGGCATCAAGGACTGGGTGACGCCCTATAAGATCG
  CCGTCCTGGTGCTGCTCAACGAGATGGGCCGCACGGGCGAGGGCGCCGTCAGCCTCGTGGAGCGGCGGAA
  GCTCAACCAGCTGCTCCTGCCCCTGCTGCAGGGCCCAGATATTACACTGTCAAAGCTGTACAAGCTAATT
  GAAGAATCGTGTCCTCAGCTGGCAAATTCAGTGCAGATCAGAATCAAGCTCATGGCTGAAGGCGAACTGA
  AGGATATGGAACAATTTTTTGATGACCTTTCAGATTCTTTTTCTGGAACTGAACCAGAGGTTCACAAAAC
  GAGTGTAGTAGGTCTGTTCCTGCGCCACATGATCTTGGCCTACAGTAAGCTTTCTTTCAGTCAAGTGTTT
  AAGCTGTACACTGCCCTCCAGCAGTACTTCCAGAACGGGGAGAAAAAGACGGTGGAAGATGCTGATATGG
  ACCGAGAGGATGGAGAGAAACAGATGGAGAAGGAAGAGCTCGACGTGTCCGTGAGAGAAGAGGAAGTATC
  TTGCAGTGGTCCTCTGTCCCAAAAACAAGCGGAATTTTTTCTCTCTCAGCAGGCCGCTTTGTTGAAGAAT
  GATGAGACTAAAGCCCTCACCCCAGCTTCCTTGCAGAAGGAATTGAACAACCTGTTGAAATTTAATCCTG
  ATTTTGCTGAAGCTCATTACCTCAGCTACTTAAACAACCTCCGTGTTCAAGATGTTTTCAGCTCAACACA
  CAGCCTCCTGCATTATTTTGACCGCCTGATTCTCACTGGAGCGGAGGGCAAAAGTAATGGGGAAGAGGGT
  TATGGCCGGAGCCTGAGATACGCTGCTCTCAACCTGGCTGCCCTGCACTGCCGCTTCGGTCACTATCAAC
  AGGCAGAGCTCGCCCTGCAGGAGGCAATTAGGATTGCCCAGGAGTCCAACGATCACGTGTGTCTGCAGCA
  CTGTTTGAGCTGGCTTTATGTGCTGGGGCAGAAGAGAGCCGATAGCTATGTTCTGCTGGAGCACTCTGTG
  AAGAAAGCAGTACATTTTGGGTTACCGTACCTCGCCTCCCTGGGAATACAGTCCCTTGTTCAACAGAGAG
  CTTTTGCTGGGAAGACGGCCAACAAACTGATGGATGCCCTAAAGGACTCTGACCTCCTGCACTGGAAACA
  CAGCCTGTCAGAACTTATCGATATCAGCATTGCACAGAAAACGGCCATCTGGAGGCTGTACGGCCGCAGC
  ACCATGGCACTGCAACAAGCCCAGATGTTGCTGAGCATGAACAGCCTGGAGTCGCTGAATGCGGGTGTGC
  AGCAGAACAATACTGAGTCCTTTGCCGTCGCTCTCTGCCATCTTGCAGAGCTCCATGCAGAACAGGGCTG
  TTTTGCGGCTGCTGGTGAAGTATTAAAGCACTTGAAGGACCGATTTCCACCCAACAGTCAGCACGCCCAG
  TTATGGATGCTGTGTGATCAAAAAATACAGTTTGACAGAGCAATGAATGATGGCAAATTCCATTTGGCTG
  ATTCACTTGTTACAGGAATCACAGCGCTTAATGGCATAGAAGGTGTATACAGGAAAGCAGTCGTACTGCA
  GGCTCAGAACCAAATGACAGAGGCACACAAGCTACTACAGAAGTTGCTGACGTACTGTCAGAAGCTAAAG
  AACACAGAAATGGTCATCAGTGTCCTCCTATCGGTGGCAGAGCTGTACTGGCGATCTTCGTCCCCGACCA
  TCGCCATGCCTGTGCTCCTGGAAGCTCTGGCCCTCTCCAAAGAATACCGATTGCAGTACTTGGCCTCCGA
  AACTGTGCTCAACTTGGCTTATGCCCAGCTCATCCTTGGAATCCCGGAACAGGCCTTAACCCTTCTCCAC
  ATGGCTATCGAGCCCATCCTAGCCGACGGGGCTGTCCTGGACAAAGGTCGTGCCATGTTCTTAGTGTCCA
  AGTGCCAAGTGGCTTCGGCAGCGTCCTATGACCCAGTGAAGAAAGCGGAAGCTCTGGAAGCTGCCATTCA
  GAACCTCAGTGAAGCCAAGAACTACTTTGCACAAGTCGACTGCAGAGAGCGCATCAGGGATGTTGCTTAC
  TTCCAGGCCAGGCTGTACCACGCTCTTGGCAAGACCCAGGAGAGGAACCATTGTGCCATGATCTTCCGGC
  AGCTGCACCAGGAGTTGCCCGCCCATGGGGTGCCCTTGATTAACCACCTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG210396 representing NM_021505
  Red=Cloning site Green=Tags(s)
  
  MMTNGVVHANLFGIKDWVTPYKIAVLVLLNEMGRTGEGAVSLVERRKLNQLLLPLLQGPDITLSKLYKLI
  EESCPQLANSVQIRIKLMAEGELKDMEQFFDDLSDSFSGTEPEVHKTSVVGLFLRHMILAYSKLSFSQVF
  KLYTALQQYFQNGEKKTVEDADMDREDGEKQMEKEELDVSVREEEVSCSGPLSQKQAEFFLSQQAALLKN
  DETKALTPASLQKELNNLLKFNPDFAEAHYLSYLNNLRVQDVFSSTHSLLHYFDRLILTGAEGKSNGEEG
  YGRSLRYAALNLAALHCRFGHYQQAELALQEAIRIAQESNDHVCLQHCLSWLYVLGQKRADSYVLLEHSV
  KKAVHFGLPYLASLGIQSLVQQRAFAGKTANKLMDALKDSDLLHWKHSLSELIDISIAQKTAIWRLYGRS
  TMALQQAQMLLSMNSLESLNAGVQQNNTESFAVALCHLAELHAEQGCFAAAGEVLKHLKDRFPPNSQHAQ
  LWMLCDQKIQFDRAMNDGKFHLADSLVTGITALNGIEGVYRKAVVLQAQNQMTEAHKLLQKLLTYCQKLK
  NTEMVISVLLSVAELYWRSSSPTIAMPVLLEALALSKEYRLQYLASETVLNLAYAQLILGIPEQALTLLH
  MAIEPILADGAVLDKGRAMFLVSKCQVASAASYDPVKKAEALEAAIQNLSEAKNYFAQVDCRERIRDVAY
  FQARLYHALGKTQERNHCAMIFRQLHQELPAHGVPLINHL
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_021505
• ORF Size: 2220 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_021505.3
• RefSeq Size: 2760 bp
• RefSeq ORF: 2223 bp
• Locus ID: 59008
• UniProt ID: Q8BTZ4
• Cytogenetics: 5 F
• Gene Summary: Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains (By similarity).[UniProtKB/Swiss-Prot Function]