Dpp3 (NM_133803) Mouse Tagged ORF Clone

CAT#: MG210374

Dpp3 (tGFP-tagged) - Mouse dipeptidylpeptidase 3 (Dpp3)

Product Images

Circular map for MG210374

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Dpp3
• Synonyms: 4930533O14Rik; C86324; DPP III
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG210374 representing NM_133803
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGATACCCAGTACATCCTGCCCAATGACATTGGTGTGTCTAGCCTGAACTGCAGGGAGGCATTCC
  GCCTGCTGTCACCCACAGAGCGCCTCTATGCCCATCACCTGTCTAGAGCTGCTTGGTATGGGGGCCTGGC
  AGTGCTGCTCCAGACATCCCCTGAAGCCCCCTACATCTATGCCCTGCTTAGCCGCCTCTTCCGTGCCCAG
  GACCCTGACCAGCTGCGCCAACATGCCCTGGCTGAGGGTCTTACTGAAGAAGAGTACCAGGCATTCCTGG
  TCTACGCTGCAGGGGTCTACTCCAACATGGGCAACTACAAGTCCTTTGGTGACACCAAGTTTGTTCCTAA
  CCTGCCCAAGGACAAGCTGGGACGTGTGATCCTGGGGAGTAAGGCTGCCCAGCAGCGGCCAGAAGAAGTC
  AGGGACCTTTGGCAGACCTGTGGGGACCTTATGTTCTCCCTGGAACCGAGGCTTCGACATCTTGGGCTGG
  GGAAGGAGGGAGTCACCACCTATTTCTCTGGGGATTGTACCATGGAAGATGCTAAGCTGGCCCAGGACTT
  TCTGGACTCTCAGAACCTCAGTGCATACAACACTCGGCTCTTCAAGGTCGTTGACCAGGAAGGGAAGTCC
  CACTATGAGGTGCGGCTGGCTTCAGTCCTCAACACAGACCCTGCTCTCGACTCTGAATTGACCTCCAAGC
  TGAAGAGATATGAGTTCCAGGGGAACCATTTCCAGGTCACCCGGGGGGACTATGCCCCCATCCTCCAGAA
  GGTGGTAGAACACTTGGAGAAAGCTAAGGCTTATGCAGCCAACAGCCACCAGGAGCAAATGCTGGCTCAG
  TACGTGGAGAGCTTCACCCAGGGCTCCATTGAGGCCCATAAGAGGGGCTCCCGCTTCTGGATTCAGGACA
  AAGGCCCCATTGTAGAGAGTTACATTGGCTTCATTGAGAGCTACCGTGACCCCTTTGGCTCCCGAGGAGA
  GTTTGAAGGCTTCGTGGCCATGGTAAACAAAGCCATGAGTGCCAAGTTCGAGCGGCTGGTCGCGAGTGCT
  GAGCAGCTGCTGAAGGAGCTGCCCTGGCCTCTTGCCTTCGAGAAGGACAAGTTCCTCACCCCTGACTTCA
  CCTCCCTGGACGTCCTCACCTTTGCTGGCTCTGGCATTCCTGCGGGCATCAACATCCCCAACTACGATGA
  CCTGAGACAGACAGAAGGCTTTAAGAATGTATCGCTGGGGAATGTGCTGGCTGTGGCCTATGCCGCAAAG
  CGGGAGAAGCTCACGTTCTTGGAGGAGGAAGACAAGGACCTGTATATCCGCTGGAAGGGGCCGTCCTTTG
  ATGTGCAGGTGGGGTTACACGAACTGTTGGGCCATGGCAGCGGCAAGCTCTTTGTACAGGATGAGAAAGG
  TGCTTTCAACTTTGACAAGGAGACTGTGATCAACCCAGAGACTGGGGAACAGATCCAGAGCTGGTACCGG
  AGTGGAGAGACGTGGGATAGCAAATTCAGCACCATTGCCTCCAGCTACGAAGAGTGCCGGGCCGAGAGTG
  TGGGCCTCTACCTCTGTCTCAACCCCCAAGTGCTGGAGATCTTTGGCTTTGAGGGAGCTGATGCAGAAGA
  TGTAATCTACGTGAACTGGCTCAACATGGTTCGGGCTGGCTTGTTGGCTTTGGAGTTCTACACCCCAGAG
  GCAGCCAACTGGCGACAGGCCCACATGCAGGCCCGGTTTGTGATCCTGCGGGTTTTGCTGGAAGCTGGCG
  AAGGACTCGTTACCGTCACTCCCACCACAGGCTCTGATGGGCGCCCTGATGCCCGGGTCCGCCTGGACCG
  CAGCAAGATCCGGTCAGTGGGCAGACCTGCCTTGGAGCGGTTCCTGCGGAGACTCCAGGTGCTGAAGTCC
  ACGGGGGACGTGGTCGCGGGGCGGGCCCTGTACGAGGGCTATGCAGCAGTCACTGACGCACCTCCAGAGT
  GCTTCCTTACCCTAAGGGATACGGTACTGCTGCGCAAGGAGTCCCGGAAGCTTATTGTTCAACCCAACAC
  TCGCCTTGAAGGCTCAGAAGTACAACTTGTGGAGTATGAGGCCTCAGCCGCTGGCCTCATCCGCTCCTTC
  TGTGAGCGGTTTCCCGAGGATGGGCCCGAGTTGGAGGAGGTTCTTATCCAGCTGGCTGCAGCAGATGCCC
  GATTCTGGAGGAACCAGGCCCAGGAGGCCCCACCTGGCCAGGCT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG210374 representing NM_133803
  Red=Cloning site Green=Tags(s)
  
  MADTQYILPNDIGVSSLNCREAFRLLSPTERLYAHHLSRAAWYGGLAVLLQTSPEAPYIYALLSRLFRAQ
  DPDQLRQHALAEGLTEEEYQAFLVYAAGVYSNMGNYKSFGDTKFVPNLPKDKLGRVILGSKAAQQRPEEV
  RDLWQTCGDLMFSLEPRLRHLGLGKEGVTTYFSGDCTMEDAKLAQDFLDSQNLSAYNTRLFKVVDQEGKS
  HYEVRLASVLNTDPALDSELTSKLKRYEFQGNHFQVTRGDYAPILQKVVEHLEKAKAYAANSHQEQMLAQ
  YVESFTQGSIEAHKRGSRFWIQDKGPIVESYIGFIESYRDPFGSRGEFEGFVAMVNKAMSAKFERLVASA
  EQLLKELPWPLAFEKDKFLTPDFTSLDVLTFAGSGIPAGINIPNYDDLRQTEGFKNVSLGNVLAVAYAAK
  REKLTFLEEEDKDLYIRWKGPSFDVQVGLHELLGHGSGKLFVQDEKGAFNFDKETVINPETGEQIQSWYR
  SGETWDSKFSTIASSYEECRAESVGLYLCLNPQVLEIFGFEGADAEDVIYVNWLNMVRAGLLALEFYTPE
  AANWRQAHMQARFVILRVLLEAGEGLVTVTPTTGSDGRPDARVRLDRSKIRSVGRPALERFLRRLQVLKS
  TGDVVAGRALYEGYAAVTDAPPECFLTLRDTVLLRKESRKLIVQPNTRLEGSEVQLVEYEASAAGLIRSF
  CERFPEDGPELEEVLIQLAAADARFWRNQAQEAPPGQA
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_133803
• ORF Size: 2214 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_133803.1, NP_598564.1
• RefSeq Size: 2654 bp
• RefSeq ORF: 2217 bp
• Locus ID: 75221
• UniProt ID: Q99KK7
• Cytogenetics: 19 A
• Gene Summary: Cleaves and degrades bioactive peptides, including angiotensin, Leu-enkephalin and Met-enkephalin. Also cleaves Arg-Arg-beta-naphthylamide (in vitro).[UniProtKB/Swiss-Prot Function]