Zfp184 (NM_183014) Mouse Tagged ORF Clone

CAT#: MG210372

Zfp184 (tGFP-tagged) - Mouse zinc finger protein 184 (Kruppel-like) (Zfp184)

Product Images

Circular map for MG210372

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Zfp184
• Synonyms: 4930500C15Rik
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG210372 representing NM_183014
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGGTCTGTCCTTTGCAGACTCTGCCAGTCTCCACGAGGGACGTCCCCTACTCCTACCATCTAGTT
  TTCGGGAATCAGTGACCTTCAAGGATGTGGTCGTGAACTTCACTCAGGAAGAATGGAAACACCTGGATCC
  TATACAGAGAGATTTGTTCAGAGATGTGACACTGGAAAATTATACACATCTGGTCTCTATAGGGCTCCAG
  GTTTCCAAACCTGATATGATTTCCCAACTAGAGCAAGGGACAGAGCCATGGACTGAGGATTCGTGCATTC
  CTGTCGGTCCCTTGGAAGACTGGAAGAAAAGAGCTGGAAATAGTGTGTCATCCCTAGAACTTGACATTTC
  TGAAGAGCATCTATTCTCAGAGACAGTAGTAACCAACAGCAAAAGGGATGATGGGAGTTTAGAGAAGCTT
  CAAGCAAACCAACAGATGCTGCCAAGGGAAGTCCAAATAACGGAGAAAACAGCACCCACTTGTGAGAGCA
  ACCTCAGTGTGAGCTCAAGCTTCATAACACAAACGGAAGTAGCTCTCGATCAGCCTTCCACTAAAACAAG
  AGCCAAGCAGAATTCCCACCCAGTTAAAAAGGAGAAGCTGTGTAAGTGCAACGAATGTGGTAAAGCTTTT
  ACTTACTGTTCTGCTCTCATTCGCCACCAAAGAACGCATACTGGAGAGAAACCCTATAAATGTAATGAAT
  GTAACAAAGCTTTCAGCAGAAGTGAAAACCTTATAAACCATCAGAGAATCCATACGGGAGATAAACCGTA
  CAAATGTGACCAGTGTGGAAAGGGTTTCATTGAGGGTCCATCTCTCACTCAACATCAAAGAATTCACACT
  GGAGAAAAGCCCTATAAATGTGATGAATGTGGGAAGGCCTTTAGTCAGAGGACCCATCTTGTTCAGCATC
  AGAGAATTCACACTGGTGAGAAGCCATATACTTGTACCGAGTGTGGGAAATCCTTCAGCCAGAGAGGTCA
  CTTTATGGAGCACCAGAAAATTCATACAGGAGAAAAACCTTTCAAGTGTGAGGAGTGTGAGAAAACCTTC
  ACCAGGAGCACACACCTCACTCAACATCAGAAAATTCACACTGGGGAGAAAACTTACAAGTGTAATGAAT
  GTGGGAAGGCCTTCAACGGCCCATCAACGTTTATCCGTCACCATATGATTCATACTGGAGAAAAGCCTTA
  TGAGTGTAATGAATGTGGCAAAGCCTTCAGCCAGCACTCCAACCTGACTCAGCATCAGAAAACACACACT
  GGGGAGAAACCCTATGACTGTGCAGAATGTGGGAAAGCCTTTAGTTACTGGTCATCCCTAGCTCAGCACC
  TGAAAATTCATACAGGAGAGAAGCCCTACAAGTGTAGCGACTGCGGGAAGGCCTTCAGTTACTGCTCCTC
  CCTTACTCAGCATCGGAGGATTCACACCCGAGAGAAGCCCTTTGAGTGCAGTGAGTGTGGAAAGGCTTTC
  AGCTATCTGTCAAACCTTAATCAGCATCAGAAAACTCACACCCAGGAGAAAGCCTATGAATGTAAGGAAT
  GTGGGAAAGCCTTTATTCGGAGTTCATCTCTTGCTAAGCATGAAAGAATTCATACTGGAGAGAAGCCTTA
  TCAATGTCATGAATGTGGAAAAACCTTCAGCTATGGCTCATCTCTTATTCAGCATAAGAAAATCCATACT
  GGTGAAAGACCTTACAAGTGTAATGAATGTGGGCGAGCCTTCAACCAGAAAATACACCTTACACAACACA
  AGAGAATTCATACAGGAGCAAAGCCGTATGCCTGCCCCAAGTGCGGGAAGACCTTTCGACACTGTTCATC
  TCTTGCTCAACATCAAAAAACTCACACAGAAGAAAAACCCTACCAGTGTAACAAATGTGAAAAAACCTTC
  AGCCAGAACTCCCGTCTGACTCAGCATCAGCGAATTCATACGGGAGAGAAGCCTTATAAGTGCAGTGAAT
  GTGACAAATGCTTTACCGGGAGCGTGCACCTGACTGAACATCGGAGTACTCACACTGGAGAGAAACCTTA
  CAACTCTGAGTGCCCACAGACTTTCAGCCAGAGCACATACCTCACTCAGCATCAGAAGATTCATTCAGGA
  GAGAAGCTTTTGGGGTGTGAGGACTGTGAAAAAGCCTTCCAGTGTCACTCTGCTCTCACCAAACACCAGA
  GATTGCACCCTGCTGTGGCTGCTGTAGGAACATCATTAACA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG210372 representing NM_183014
  Red=Cloning site Green=Tags(s)
  
  MAGLSFADSASLHEGRPLLLPSSFRESVTFKDVVVNFTQEEWKHLDPIQRDLFRDVTLENYTHLVSIGLQ
  VSKPDMISQLEQGTEPWTEDSCIPVGPLEDWKKRAGNSVSSLELDISEEHLFSETVVTNSKRDDGSLEKL
  QANQQMLPREVQITEKTAPTCESNLSVSSSFITQTEVALDQPSTKTRAKQNSHPVKKEKLCKCNECGKAF
  TYCSALIRHQRTHTGEKPYKCNECNKAFSRSENLINHQRIHTGDKPYKCDQCGKGFIEGPSLTQHQRIHT
  GEKPYKCDECGKAFSQRTHLVQHQRIHTGEKPYTCTECGKSFSQRGHFMEHQKIHTGEKPFKCEECEKTF
  TRSTHLTQHQKIHTGEKTYKCNECGKAFNGPSTFIRHHMIHTGEKPYECNECGKAFSQHSNLTQHQKTHT
  GEKPYDCAECGKAFSYWSSLAQHLKIHTGEKPYKCSDCGKAFSYCSSLTQHRRIHTREKPFECSECGKAF
  SYLSNLNQHQKTHTQEKAYECKECGKAFIRSSSLAKHERIHTGEKPYQCHECGKTFSYGSSLIQHKKIHT
  GERPYKCNECGRAFNQKIHLTQHKRIHTGAKPYACPKCGKTFRHCSSLAQHQKTHTEEKPYQCNKCEKTF
  SQNSRLTQHQRIHTGEKPYKCSECDKCFTGSVHLTEHRSTHTGEKPYNSECPQTFSQSTYLTQHQKIHSG
  EKLLGCEDCEKAFQCHSALTKHQRLHPAVAAVGTSLT
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_183014
• ORF Size: 2211 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_183014.1, NP_898835.1
• RefSeq Size: 2730 bp
• RefSeq ORF: 2214 bp
• Locus ID: 193452
• UniProt ID: Q7TSH9
• Cytogenetics: 13 A3.1
• Gene Summary: May be involved in transcriptional regulation.[UniProtKB/Swiss-Prot Function]