Mycbpap (NM_170671) Mouse Tagged ORF Clone

CAT#: MG210214

Mycbpap (tGFP-tagged) - Mouse Mycbp associated protein (Mycbpap)

Product Images

Circular map for MG210214

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Mycbpap
• Synonyms: 4932408B01Rik; AMAP-1; AW125474
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG210214 representing NM_170671
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATGAAGAAGATTACTGAAAGGCAGTCCCCGCTCAAGTTGCTCGAAAAGAAGCGGGCGAAGGCACCTG
  AACAGCCCACTCCCCCCATTCAGGAAGAGCCTGAGCCTGTCAGCAATGTCTTACAAGGAGATGACATTCT
  TGCCTTGGCCATTAAAAAGGAAGACTTGAGGAAGCAACATGTTCCTCAGTTTGAAGAAACAGGAGAAAAG
  CCTGTGGTCACACAGAAATTTATCATCCGTAAGCTCAAACCGAAAGACTCTAGCAAAAGGGTCTACCACT
  TGGTAGCACATCCTGCTACTCCAGATGCCGCCACCAAGCCTCTGGACTACTCCGGTCCTCATGACAGCTT
  CTTAAGCAGTGGCCAGATCCTCCCTCACCAGATCTTGGGAAGTCTCCAGGACTTTAAAAGAATCGCGGTT
  GCTCGAGGGAACACCCAGCTGGCTAAGCTGATTCACATCCAGCCCTGCCTGATGACCCTCATCTCAGCCA
  AAGAAGAGCCAAAGCCAAAGCCCCCCAAGGAGGAGGAGAGACCTTCTCCATGGGCCCCACCTCCTCAGCA
  CAACTTCCTAAAGAACTGGAGACGGCACATTGCCTTGCGGAAGAAGCAACAGGAAGCTCTCAGCAAGCAC
  CTGAAGAAGCCAGCCAGTGAGCTGTTGATGCATACCGGGGAGAGCTATAGGAAGATCCAGGAGGAGCGCG
  AGGTCATTGACCGTGCACTTCCCACCCAGCATGACGGAAAAGCAACCAGTTGGTTCTGGAGTCCACTGGA
  ATACCTGGGTGATGAGATGACAGGTCTTCTCATGACCAAAAAGAAAACTCAGCGTGGTCTGGTGGAACCC
  ATCACACACATCAGGAAGCCCCTCTCCATCCAAGTGGAGACTGGATTACCAGCCCAGAAGGATGCTTGGT
  ACCGCTACACCTGGGACCGGAGTCTGTTTCTGATCTACCGACGCAAGGAGCTGCAGAGTATCATGGCAGA
  GCTGGATTTCAGCCAGCAGGATATTGATGGCCTGGAGGTGGTGGGCCATGGGAAGCCCTTCTCATCTGTC
  ACAGTGGAAGAACATTTGCCACCTGAAAAGATCCAGAAGAGCTCCTCGGAAGACACGGTATTTCTAGACT
  CCTTGACCAACCTCTCCGACATGGTCCCCATGCCTATTCTCGGCCCTTCGCTGCTGTTCTGTGGGAAGCC
  AGCTTGCTGGGTCAGAGGCAGTAACCCAGAGGACAAGAAAAACATCGGAATTGGTGTCCGCCTGACATTT
  GAAACCCTTGAAGGGGAGAGAACGTCCTCAGAACTGACTGTGGTCAATAACGGCACGGTGGCCATTTGGT
  ACAACTGGCGAAGGCGGCCCCACCAGGATTTTTTCCAAGACCTAAAGCAAAACAAGACGCAGAGGTTTTA
  CTTTAACAACCGGGAAGGTGTGATTCTGCCCGGGGAAACCAAACACTTTACCTTCTTCTTCAAGTCTCTG
  AATGCTGGCATCTTCAGGGAATCTTGGGAGTTTGGGACCCACCCTACACTCCTGGGAGGTGCTGTCCTTC
  AGGTCACCCTCCATGCCATCTCACTGACCCAGGACATTTTTATGGATGAGAGGAAGTTGTTGGAGACAAA
  GCTGGCTGCCCACGAGGCCATCACCATTGCCCAGAGCGTGCTGCAGGACTTACTGAGGGGCATCTCCACG
  CCAGAGCGCACACCATCCCCGGTGGACGCGTATCTCACCGAGGAGGACTTGTTCAACTACAGGAATCCTA
  GGTTGCATTACCAGCATCAAGTGGTGCAAAACTTGCACCAGCTGTGGCAACAGTACAGGAAGGCCAAGGC
  AACCCAGAAGGAAACCCCCAGCCTCAGAACTCCAGTGCCGTTACTGTTGGTGGAGAAGGCCTCAGGTTCG
  ATAAGCCCCAGGAACCTGGTCTCAGAGTACTCGCAGCTGTCACCGCATCAGGAGATGGACACCGCCAGGA
  AGACTCGGGACTTCTTCCTGTCTCTGAAGTCTAGCATAGGAAAGAAGAGTGTGGCACGGAAGAGCATTAT
  GGAGGAACTGCTGGTGGAGGAGGGTCCGGACAGGGAGACCACCCAGCGCCCCTGGGCTCTGAAAAGCATT
  TCCCCGCCTAAGTGGAACCTGTGTCTAGAGGACTTCAGACAGGCAGTGATGACCTTCCCCGAGGAGCTCC
  AGAGAGAGGATGCCCTCATCCAGCTCAATAAGGCGGCAATGGAGCTGTGTCAGGAGCAGAAGCCACTGCA
  GTCGGACCTCCTATACCAGATGTGTCTGCAGCTGTGGCGAGACGTGATTGACAGCCTGGTAAGCCAGTCC
  CTGTGGCTAAGAAATCTTCTGGGCCTGCCTGAGAAGGAGACGGTTTATTTGGATCTTCCAGATGAACAAG
  GTCAAAAATCCCCTCCAGTCACAGAGTCGAAGGTGACAAGTGGGAAAGCTGGGAAGGAGGATCGGAGAGG
  GGGCGCACAGGAGAAGAAACAACTGGGTACCAAAGACAAAGATGATAAAAGGGGTTCCAAGACACCAGGG
  AAAGAGGACCGCCCGAATAGCAAAAAACTGAAGCCAAAAGATGACAAGAAAGTGGTAAAGTCTGCAAGCC
  GGGACAGGCTGCTCTCAGAAGATCCTCCCCCTGACAGCACGGCACCCTCCCAGGAGCCCATAGACCCCCT
  GGTCATGGAGAAATATACCCAGCGGTTGCACGCTGAGGTCTATGCGTTGCTGGACAACCTGGTGACAGAT
  GTGATGGTCCTGGCTGATGAGCTCAGCTCTACAAAGAATGTTGAAGAGTCTTTACGTTTTTGCAGC
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG210214 representing NM_170671
  Red=Cloning site Green=Tags(s)
  
  MMKKITERQSPLKLLEKKRAKAPEQPTPPIQEEPEPVSNVLQGDDILALAIKKEDLRKQHVPQFEETGEK
  PVVTQKFIIRKLKPKDSSKRVYHLVAHPATPDAATKPLDYSGPHDSFLSSGQILPHQILGSLQDFKRIAV
  ARGNTQLAKLIHIQPCLMTLISAKEEPKPKPPKEEERPSPWAPPPQHNFLKNWRRHIALRKKQQEALSKH
  LKKPASELLMHTGESYRKIQEEREVIDRALPTQHDGKATSWFWSPLEYLGDEMTGLLMTKKKTQRGLVEP
  ITHIRKPLSIQVETGLPAQKDAWYRYTWDRSLFLIYRRKELQSIMAELDFSQQDIDGLEVVGHGKPFSSV
  TVEEHLPPEKIQKSSSEDTVFLDSLTNLSDMVPMPILGPSLLFCGKPACWVRGSNPEDKKNIGIGVRLTF
  ETLEGERTSSELTVVNNGTVAIWYNWRRRPHQDFFQDLKQNKTQRFYFNNREGVILPGETKHFTFFFKSL
  NAGIFRESWEFGTHPTLLGGAVLQVTLHAISLTQDIFMDERKLLETKLAAHEAITIAQSVLQDLLRGIST
  PERTPSPVDAYLTEEDLFNYRNPRLHYQHQVVQNLHQLWQQYRKAKATQKETPSLRTPVPLLLVEKASGS
  ISPRNLVSEYSQLSPHQEMDTARKTRDFFLSLKSSIGKKSVARKSIMEELLVEEGPDRETTQRPWALKSI
  SPPKWNLCLEDFRQAVMTFPEELQREDALIQLNKAAMELCQEQKPLQSDLLYQMCLQLWRDVIDSLVSQS
  LWLRNLLGLPEKETVYLDLPDEQGQKSPPVTESKVTSGKAGKEDRRGGAQEKKQLGTKDKDDKRGSKTPG
  KEDRPNSKKLKPKDDKKVVKSASRDRLLSEDPPPDSTAPSQEPIDPLVMEKYTQRLHAEVYALLDNLVTD
  VMVLADELSSTKNVEESLRFCS
  
  SGPTRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_170671
• ORF Size: 2796 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_170671.2, NP_733771.2
• RefSeq Size: 2944 bp
• RefSeq ORF: 2799 bp
• Locus ID: 104601
• UniProt ID: Q5SUV2
• Cytogenetics: 11 D
• Gene Summary: May play a role in spermatogenesis. May be involved in synaptic processes (By similarity).[UniProtKB/Swiss-Prot Function]