Ssrp1 (BC042502) Mouse Tagged ORF Clone

CAT#: MG210205

Ssrp1 (tGFP-tagged) - Mouse structure specific recognition protein 1 (cDNA clone MGC:30412 IMAGE:5005209)

Product Images

Circular map for MG210205

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Ssrp1
• Synonyms: C81323; Hmg1-rs1; Hmgi-rs3; Hmgox; T160
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG210205 representing BC042502
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGAGACATTGGAGTTCAACGACATCTTCCAGGAGGTGAAAGGGTCCATGAATGATGGGAGGCTTC
  GATTGAGCCGTCAGGGTATCATCTTTAAGAACAGCAAGACGGGCAAAGTGGACAACATCCAGGCTGGGGA
  GTTGACAGAAGGCATCTGGCGTCGGGTAGCATTAGGCCATGGGCTTAAACTGCTCACAAAGAATGGGCAT
  GTCTACAAGTACGATGGCTTCCGCGAATCGGAGTTTGAGAAACTCTCTGACTTCTTCAAAACTCACTATC
  GCCTTGAGCTAATGGAGAAGGATCTGTGTGTGAAGGGCTGGAACTGGGGGACAGTGAAGTTTGGAGGACA
  GCTGCTTTCTTTTGACATTGGTGATCAACCAGTCTTTGAGATACCCCTAAGCAATGTGTCCCAGTGTACC
  ACAGGCAAGAATGAGGTGACCCTGGAATTCCACCAGAATGACGATGCTGAAGTATCTCTCATGGAGGTGC
  GCTTCTATGTTCCTCCCACGCAGGAAGATGGTGTGGACCCTGTGGAGGCCTTTGCCCAGAATGTTCTGTC
  AAAGGCAGATGTGATCCAGGCCACCGGAGACGCCATCTGCATCTTCCGGGAGCTGCAGTGTTTGACTCCT
  CGCGGTCGATACGATATCCGGATCTACCCTACCTTTCTACACCTGCATGGCAAGACCTTTGACTACAAGA
  TCCCCTATACTACAGTTCTCCGTCTCTTCCTGCTACCACACAAGGATCAGAGACAGATGTTCTTTGTGAT
  CAGCTTGGATCCTCCCATCAAGCAGGGCCAAACTCGTTACCACTTCCTGATCCTCCTCTTCTCCAAGGAT
  GAGGACATCTCCTTGACTCTCAACATGAATGAGGAAGAAGTAGAAAAGCGCTTTGAGGGGCGACTCACCA
  AGAACATGTCAGGATCCCTCTATGAAATGGTCAGTCGGGTCATGAAAGCACTTGTCAACCGTAAAATCAC
  AGTCCCAGGCAACTTCCAAGGGCACTCAGGGGCCCAGTGTATTACCTGCTCCTATAAGGCCAGCTCAGGA
  CTCCTGTACCCACTGGAGCGGGGCTTCATCTACGTGCATAAGCCCCCTGTGCACATCCGCTTTGATGAGA
  TCTCTTTTGTCAACTTTGCCCGTGGCACCACGACCACTCGTTCCTTCGACTTTGAGATTGAGACCAAGCA
  AGGCACTCAGTATACCTTCAGCAGCATTGAAAGGGAGGAGTATGGAAAGCTTTTTGATTTTGTCAATGCG
  AAAAAGCTCAACATCAAGAACAGAGGACTGAAAGAGGGCATTAACCCAGGCTATGACGATTATGCTGACT
  CTGATGAAGACCAGCATGATGCCTATTTGGAGAGGATGAAGGAGGAGGGCAAGATCCGGGAGGAGAATGC
  CAATGACAGCAGCGACGACTCAGGAGAAGAGACTGATGAGTCCTTCAATCCTGGTGAAGAAGAAGAAGAT
  GTGGCAGAGGAGTTTGACAGCAATGCCTCTGCCAGCTCCTCCAGCAATGAGGGTGACAGTGACCGTGAAG
  AGAAGAAACGGGAACAGCTCAAAAGGGCTAAGATGGCCAAGGATCGAAAGAGCCGCAGGAAGTCTTCAGA
  GGCAAAGAAGGGTAAAGATCCAAACGCCCCAAAGAGGCCTATGTCTGCGTACATGCTGTGGCTTAATGCA
  AGCCGCGAGAAGATCAAGTCGGATCATCCTGGCATCAGTATCACAGATCTTTCCAAGAAGGCAGGGGAAA
  TCTGGAAGGGAATGTCCAAAGAGAAGAAAGAGGAGTGGGACCGCAAGGCTGAGGATGCTAGGAGGGAGTA
  TGAGAAAGCCATGAAAGAGTATGAAGGAGGCAGAGGGGACTCGTCTAAAAGGGACAAGTCTAAGAGGGAC
  AAGTCTAAGAAGAAAAAGAAAGTAAAAGCAAAGATGGAAAAAAAGTCCACTCCTTCCCGGGGCTCGTCAT
  CCAAGTCTTCATCCAGGCAGTTGAGTGACAGCTTCAAGAGCAAAGAGTTTGTGTCCAGTGATGAGAGCTC
  TTCAGGCGAGAACAAGAGCAAAAAGAAGAGGAGGCGGAGCGAGGACTCTGAAGAGGAGCTAGCCAGTACC
  CCCTCCAAGCTCAGAGGACTCTGCCTCGGGATC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG210205 representing BC042502
  Red=Cloning site Green=Tags(s)
  
  MAETLEFNDIFQEVKGSMNDGRLRLSRQGIIFKNSKTGKVDNIQAGELTEGIWRRVALGHGLKLLTKNGH
  VYKYDGFRESEFEKLSDFFKTHYRLELMEKDLCVKGWNWGTVKFGGQLLSFDIGDQPVFEIPLSNVSQCT
  TGKNEVTLEFHQNDDAEVSLMEVRFYVPPTQEDGVDPVEAFAQNVLSKADVIQATGDAICIFRELQCLTP
  RGRYDIRIYPTFLHLHGKTFDYKIPYTTVLRLFLLPHKDQRQMFFVISLDPPIKQGQTRYHFLILLFSKD
  EDISLTLNMNEEEVEKRFEGRLTKNMSGSLYEMVSRVMKALVNRKITVPGNFQGHSGAQCITCSYKASSG
  LLYPLERGFIYVHKPPVHIRFDEISFVNFARGTTTTRSFDFEIETKQGTQYTFSSIEREEYGKLFDFVNA
  KKLNIKNRGLKEGINPGYDDYADSDEDQHDAYLERMKEEGKIREENANDSSDDSGEETDESFNPGEEEED
  VAEEFDSNASASSSSNEGDSDREEKKREQLKRAKMAKDRKSRRKSSEAKKGKDPNAPKRPMSAYMLWLNA
  SREKIKSDHPGISITDLSKKAGEIWKGMSKEKKEEWDRKAEDARREYEKAMKEYEGGRGDSSKRDKSKRD
  KSKKKKKVKAKMEKKSTPSRGSSSKSSSRQLSDSFKSKEFVSSDESSSGENKSKKKRRRSEDSEEELAST
  PSKLRGLCLGI
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC042502
• ORF Size: 2133 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC042502.1
• RefSeq Size: 2792 bp
• RefSeq ORF: 2135 bp
• Locus ID: 20833
• Cytogenetics: 2 49.45 cM
• Gene Summary: Component of the FACT complex, a general chromatin factor that acts to reorganize nucleosomes. The FACT complex is involved in multiple processes that require DNA as a template such as mRNA elongation, DNA replication and DNA repair. During transcription elongation the FACT complex acts as a histone chaperone that both destabilizes and restores nucleosomal structure. It facilitates the passage of RNA polymerase II and transcription by promoting the dissociation of one histone H2A-H2B dimer from the nucleosome, then subsequently promotes the reestablishment of the nucleosome following the passage of RNA polymerase II. The FACT complex is probably also involved in phosphorylation of 'Ser-392' of p53/TP53 via its association with CK2 (casein kinase II). Binds specifically to double-stranded DNA. Also acts as a transcriptional coactivator for p63/TP63.[UniProtKB/Swiss-Prot Function]