Maml3 (NM_001004176) Mouse Tagged ORF Clone

CAT#: MG209988

Maml3 (tGFP-tagged) - Mouse mastermind like 3 (Drosophila) (Maml3)

Product Images

Circular map for MG209988

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Maml3
• Synonyms: AV234550; BC049812; Mam-2; mKIAA1816
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG209988 representing NM_001004176
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCCCAGCAGAGCAGCTCAAACAGATGGCTGCCCAGCAACAACAGAGGGCAAAACTCATGCAGCAGA
  AGCAACAGCAGCAGCAGCAACAGCAGCAGCAGCAGCAGCAACAACAACAACAACAGCAGCACTCAAATCA
  GACTTCAAGTTGGTCTCCCTTAGGGCCTCCGTCAAGTCCATATGGAACAGCTTTTGCTTCAGAAAAACCA
  AATAGCCCAATGATGTACCCCCAAGCCTTTAACAACCAAAGCACTATAGTGCCTGCAATGGCAAACAGCC
  TGCAGAAGACGACCATGAACAACTACCTCCCTTCCAATCACATGAATATGATCAGTCAGCAGCCAAATAA
  CTTGGGTACAAACTCCTTAAACAAACAGCACAATATTCTCACCTATGGCAACACTAAGCCTCTGACCCAT
  TTTAATGCGGACTTGAGTCCGAGAATGACGCCCCCTATGGCCAACCCCAACAAAACCCCCTTGATGCCCT
  ACATCCAGCAGCCTCAGCAGTCGCAGCAGCCCCAGCCGCAGCCGCCCCAGCAGCAGCCACCGCCTCCCCC
  GCAGCTGCAGGCTCCCAGGGCGCACCTGAGCGAGGACCAGAAACGCATGCTTCTCATTAAGCAGAAAGGA
  GTGATGAACCCACCCATGGCCTATGCTGCGCTTCCTGCCCACGGTCAGGAGCAGCACGCAGTCGGTATTC
  CCCGGACCACAGGCCCTATGCAGAACTCTGTGCCCTCGGGGTCGGGCAGCATGGTCTCCGGAGCCAGTCC
  TGGGGGCCTTGGATTTCTAGGCAGCCAGCCACAGGCAGCCATCATGAAGCAGATGCTAATGGACCAACGG
  GCCCAGCTGATGGAGCACCAGAAGCAACAGTTCCTTCGGGAGCAGAGGCAGCAGCAGCAACAGCAGCAGC
  AGCAGCAACAGCAGCAGCAGCAGCAGCAGCAGATCCTGGCTGAGCAGCAGTTACAGCAGCCGCACCTGCC
  CCGTCAGCACCTCCAGCAACAGCGGAACCCATACCCAGTGCAGCAGGTCAACCAGTTTCAAGGTTCACCC
  CAGGACATAGCAGCTGTGAGAAACCAGGTGGCTCTCCAGAGCATGCGAGCATCGAGGTTGATTGCACAGA
  ATGCGGGCATGATGGGAATGGGACCCTCCCAGAACCCAGGGACGATGGCTACGGCAGCAGCGCAGTCAGA
  GATAGGTCTGGCCTCTTACAGTGCCCCTCCAACCAGCCAACCAGGAATGTACAACATGAACACAGGAATG
  ACCCAAATGTTGCAGCATTCCAATCAGAGTGGAATGGGCATCCCACACAACCAATCCCAGGGACCCAGGC
  CGCCAGCCTCCAGCCAGGGGGTCGGCATGGTGAGCGGCTTCGGTCAGAGCATGCTGGTGAACTCGGCTCT
  CTCGCAGCAGCACCAGCAGCTGAAAGGGTCTGTGGGCCAGGCCTTGCCTAGGCCCCAGGGCCCTCCAAGG
  CTTCAGAGTGTCATGGGAACCGTCCAGCAAGGAGCACAAAACTGGCAACAGAGGAGCTTACAAGGGGTGC
  CTGGGAGGACTAGTGGGGAGTTGGGACCATTCAACAATGGCGCCAGCTACCCATTGCAAGCCGGTCAGCC
  AAGACTGACCAAACAGCACTTCCCACAGGGGCTGAGCCAGCCCGTCATGGATGCTAACACTGGCGCAGTG
  AGAACCCTCAACCCAGCAGCTATGGGACGTCAGATGATGCCAACCCTGGCAGGACAGCAGAGTGCCAGCC
  AGGTGAGGCCATTGGTCATGCCTGGTCTGAGCCAGGGTGTCCCTGGCATGCCAGCATTCAGCCAGCCCCC
  AGCACAGCAGCAGATAGCGGGTGGCAACTTTGCTGCGAGCAACCAGGGCCAGGCCTATGAGCGGACCCCT
  GCTCAGGACATGTCATACAGTTATAGCGGCGAAGGTGTCGGGGCTGCCTTCCCTGGCCTCCCAGACAGTA
  CAGACCTCGTGGACTCCATCATCAAAAGTGGCCCAGGGGATGAATGGATGCAAGAGCTTGATGAACTGTT
  TGGTAACCCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG209988 representing NM_001004176
  Red=Cloning site Green=Tags(s)
  
  MSPAEQLKQMAAQQQQRAKLMQQKQQQQQQQQQQQQQQQQQQQHSNQTSSWSPLGPPSSPYGTAFASEKP
  NSPMMYPQAFNNQSTIVPAMANSLQKTTMNNYLPSNHMNMISQQPNNLGTNSLNKQHNILTYGNTKPLTH
  FNADLSPRMTPPMANPNKTPLMPYIQQPQQSQQPQPQPPQQQPPPPPQLQAPRAHLSEDQKRMLLIKQKG
  VMNPPMAYAALPAHGQEQHAVGIPRTTGPMQNSVPSGSGSMVSGASPGGLGFLGSQPQAAIMKQMLMDQR
  AQLMEHQKQQFLREQRQQQQQQQQQQQQQQQQQQILAEQQLQQPHLPRQHLQQQRNPYPVQQVNQFQGSP
  QDIAAVRNQVALQSMRASRLIAQNAGMMGMGPSQNPGTMATAAAQSEIGLASYSAPPTSQPGMYNMNTGM
  TQMLQHSNQSGMGIPHNQSQGPRPPASSQGVGMVSGFGQSMLVNSALSQQHQQLKGSVGQALPRPQGPPR
  LQSVMGTVQQGAQNWQQRSLQGVPGRTSGELGPFNNGASYPLQAGQPRLTKQHFPQGLSQPVMDANTGAV
  RTLNPAAMGRQMMPTLAGQQSASQVRPLVMPGLSQGVPGMPAFSQPPAQQQIAGGNFAASNQGQAYERTP
  AQDMSYSYSGEGVGAAFPGLPDSTDLVDSIIKSGPGDEWMQELDELFGNP
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001004176
• ORF Size: 2040 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001004176.1, NP_001004176.1
• RefSeq Size: 6514 bp
• RefSeq ORF: 3408 bp
• Locus ID: 433586
• Cytogenetics: 3 C