Hspa9 (NM_010481) Mouse Tagged ORF Clone

CAT#: MG209980

Hspa9 (tGFP-tagged) - Mouse heat shock protein 9 (Hspa9)

Product Images

Circular map for MG209980

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Hspa9
• Synonyms: 74kDa; Csa; Grp75; Hsc74; Hsp74; Hsp74a; Hspa9a; Mortalin; Mot-2; Mot2; Mthsp70; Pbp74
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG209980 representing NM_010481
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATAAGCGCCAGCAGAGCCGCGGCCGCGCGTCTCGTGGGCACCGCTGCGTCCCGGAGCCCCGCAGCCG
  CCCGTCCCCAGGATGGCTGGAATGGCCTTAGCCATGAGGCTTTTAGATTTGTTTCAAGAAGAGATTATGC
  ATCAGAAGCAATCAAGGGTGCAGTGGTTGGTATTGATTTGGGTACTACTAACTCCTGTGTGGCTGTTATG
  GAGGGCAAACAAGCAAAGGTCCTGGAGAATGCTGAAGGTGCCAGAACTACCCCTTCTGTGGTTGCCTTTA
  CAGCAGATGGAGAACGACTTGTTGGTATGCCAGCAAAACGGCAAGCTGTCACCAATCCAAACAATACCTT
  CTATGCTACTAAGCGTCTTATTGGACGACGATATGATGACCCTGAAGTACAGAAAGACACTAAGAATGTT
  CCTTTTAAAATTGTCCGTGCCTCCAATGGTGATGCTTGGGTTGAGGCTCATGGAAAACTCTATTCTCCAA
  GTCAGATTGGAGCATTTGTGTTGATGAAGATGAAAGAGACTGCAGAAAATTACTTGGGCCACACAGCAAA
  AAATGCTGTGATCACAGTCCCTGCTTATTTCAATGATTCACAGCGACAGGCCACTAAGGATGCTGGCCAG
  ATATCTGGGCTAAATGTGCTTCGAGTGATCAATGAGCCTACAGCTGCTGCTCTAGCTTACGGTCTGGACA
  AATCTGAAGATAAAGTCATTGCTGTGTATGATTTAGGTGGTGGAACCTTTGACATTTCTATCCTGGAAAT
  TCAGAAAGGAGTGTTTGAGGTGAAATCTACCAATGGGGACACTTTCTTAGGAGGGGAAGACTTTGACCAA
  GCTTTGTTGCGGCACATTGTCAAGGAGTTCAAGAGAGAGACAGGGGTTGATTTGACCAAAGACAACATGG
  CGCTTCAGAGGGTTCGGGAAGCTGCTGAGAAGGCTAAATGTGAACTTTCCTCATCTGTGCAGACTGACAT
  CAACTTGCCATACCTTACCATGGATGCTTCTGGACCAAAGCATTTGAATATGAAGCTGACTCGAGCTCAG
  TTTGAAGGCATTGTCACAGATCTAATCAAGAGAACTATTGCTCCGTGTCAGAAAGCTATGCAGGATGCAG
  AAGTCAGCAAGAGTGACATAGGAGAAGTGATTCTGGTTGGTGGCATGACAAGGATGCCCAAGGTTCAGCA
  GACTGTACAAGATCTTTTTGGCAGAGCCCCGAGTAAAGCTGTTAATCCTGATGAGGCTGTAGCCATCGGA
  GCTGCCATCCAGGGAGGTGTGTTGGCTGGTGACGTTACAGACGTGCTGCTCCTGGATGTCACTCCCCTCT
  CTCTGGGTATTGAGACTCTGGGAGGCGTCTTTACCAAACTTATTAATAGGAACACCACTATTCCAACCAA
  AAAGAGCCAGGTGTTTTCTACTGCTGCTGATGGACAAACTCAAGTAGAGATTAAAGTGTGTCAGGGGGAA
  CGAGAGATGGCTGGAGACAACAAACTTCTAGGACAGTTCACTTTGATTGGAATTCCCCCAGCCCCTCGTG
  GAGTGCCCCAGATTGAAGTTACATTTGACATTGATGCCAATGGGATTGTGCACGTTTCTGCCAAAGATAA
  AGGCACTGGTCGTGAGCAACAGATTGTAATCCAGTCTTCTGGTGGATTAAGCAAAGATGATATTGAAAAT
  ATGGTTAAAAATGCAGAGAAGTACGCTGAGGAAGACCGCAGGAAGAAGGAACGTGTTGAAGCAGTTAATA
  TGGCTGAAGGAATTATTCATGACACAGAAACCAAGATGGAAGAATTTAAGGACCAGTTGCCTGCTGATGA
  GTGCAACAAGCTAAAGGAAGAGATTTCCAAAATGAGAGCGCTCCTTGCTGGAAAGGACAGTGAGACAGGA
  GAGAACATCAGGCAGGCAGCATCTTCCCTACAGCAGGCGTCATTGAAACTCTTCGAAATGGCGTACAAAA
  AGATGGCATCTGAACGGGAAGGTTCTGGAAGTTCTGGCACTGGGGAACAGAAGGAAGATCAGAAGGAAGA
  GAAACAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG209980 representing NM_010481
  Red=Cloning site Green=Tags(s)
  
  MISASRAAAARLVGTAASRSPAAARPQDGWNGLSHEAFRFVSRRDYASEAIKGAVVGIDLGTTNSCVAVM
  EGKQAKVLENAEGARTTPSVVAFTADGERLVGMPAKRQAVTNPNNTFYATKRLIGRRYDDPEVQKDTKNV
  PFKIVRASNGDAWVEAHGKLYSPSQIGAFVLMKMKETAENYLGHTAKNAVITVPAYFNDSQRQATKDAGQ
  ISGLNVLRVINEPTAAALAYGLDKSEDKVIAVYDLGGGTFDISILEIQKGVFEVKSTNGDTFLGGEDFDQ
  ALLRHIVKEFKRETGVDLTKDNMALQRVREAAEKAKCELSSSVQTDINLPYLTMDASGPKHLNMKLTRAQ
  FEGIVTDLIKRTIAPCQKAMQDAEVSKSDIGEVILVGGMTRMPKVQQTVQDLFGRAPSKAVNPDEAVAIG
  AAIQGGVLAGDVTDVLLLDVTPLSLGIETLGGVFTKLINRNTTIPTKKSQVFSTAADGQTQVEIKVCQGE
  REMAGDNKLLGQFTLIGIPPAPRGVPQIEVTFDIDANGIVHVSAKDKGTGREQQIVIQSSGGLSKDDIEN
  MVKNAEKYAEEDRRKKERVEAVNMAEGIIHDTETKMEEFKDQLPADECNKLKEEISKMRALLAGKDSETG
  ENIRQAASSLQQASLKLFEMAYKKMASEREGSGSSGTGEQKEDQKEEKQ
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_010481
• ORF Size: 2037 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_010481.2, NP_034611.2
• RefSeq Size: 3067 bp
• RefSeq ORF: 2040 bp
• Locus ID: 15526
• UniProt ID: P38647
• Cytogenetics: 18 18.8 cM
• Gene Summary: Chaperone protein which plays an important role in mitochondrial iron-sulfur cluster (ISC) biogenesis (PubMed:26702583). Interacts with and stabilizes ISC cluster assembly proteins FXN, NFU1, NFS1 and ISCU (By similarity). Regulates erythropoiesis via stabilization of ISC assembly (PubMed:21123823). May play a role in the control of cell proliferation and cellular aging (PubMed:8454632).[UniProtKB/Swiss-Prot Function]