Ogfr (NM_031373) Mouse Tagged ORF Clone

CAT#: MG209623

Ogfr (tGFP-tagged) - Mouse opioid growth factor receptor (Ogfr). Note: ORF is codon optimized

Product Images

Circular map for MG209623

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Ogfr
• Synonyms: 2010013E17Rik
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG209623 representing NM_031373, codon optimized.
  Due to the complexity of NM_031373, the ORF clone is codon optimized for mammalian Expression.
  The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical.
  
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACGACCCAGAATGCGACAGCACGTGGGAAGACGAATCCGAAGAGGATGGGGAGGATGGACAGGCTG
  ACGACACTACTGATGAGGATACTGGCGATGACGATGGAGACGCTGAAGAGGCTAGGCCTTCACTGTTCCA
  GTCTCGGATGACCAGATACCGCAATTGGAGGGCGATGCAGGACATGCAAAGATACAGACACAACTACCCT
  GACCTTACCGACCAGGACTGTAACGGCGACATGTGCAATCTCTCCTTCTACAAGAACGAGATCTGTTTCC
  AGCCAAATGGGTTCTTGATCGAGGATATTCTTCAGAATTGGAAGGATAATTATGACTTGTTGGAGGAAAA
  TCATTCCTATATCCAGTGGCTCTTCCCCTTGAGGGAACCCGGCGTGAATTGGCATGCAAAACCTCTGACT
  CTTAAAGAAGTCGAGGCTTTCAAGTCCTCTAAAGAGGTCCGAGAGAGACTGGTCCGCGCTTATGAGCTGA
  TGCTGGGCTTCTATGGAATCCAGTTGGAAGATCGAAACACTGGTGCTGTTTGTCGGGCTCAGAATTTCCA
  GCCAAGATTCCATAATCTGAACTCTCACTCTCATAATAACCTGCGGATAACCAGAATTCTCAAGTCCCTC
  GGAGAGTTGGGGCTGGAACATTACCAGGCGCCCCTCGTAAGGTTCTTCCTGGAAGAAACGCTTGTGCAGC
  ACAAGTTGCCTTCAGTGCGCCAGTCTGCACTGGATTACTTCCTCTTCGCTGTACGATGTAGGCATCAGAG
  GCGGGAGCTTGTACATTTTGCTTGGGAGCACTTCAAGCCACGCCGAGAGTTCGTGTGGGGCCCAAGAGAC
  AAGCTGCGGCGGTTCAGACCCCAAACGATAAGTAGACCACTTATGGGCCTGGGGCAGGCAGACAAGGATG
  AAGGACCCGGCGACCCTTCTCAGGAGGCTGGAACTCAGGGCCGGACCTGCGGATCCGGTCGCGACCTGTC
  TGGAGACAGCGGGACCGCTGAGGATCTGTCCCTGCTCTCAGCCAAACCGCAGGATGTTGGAACGCTCGAT
  GGGGACCAGAGGCATGAGGCTAAAAGCCCGTCTCCCAAGGAGTCAAAAAAAAGAAAGTTGGAGGGGAACA
  GACAAGAACAGGTACCAGGCGAGCCCGATCCTCAGGGTGTGTCAGAGGTCGAAAAAATCGCCTTGAATCT
  CGAGGGCTGTGCCCTGTCACCCACTTCCCAGGAGCCTAGGGAAGCCGAACAGCCCTGTCTGGTCGCTCGA
  GTGGCCAACGAAGTCAGGAAGCGGAGGAAGGTGGAAGAGGGTGCAGAGGGGGATGGTGTGGCCTCCAATA
  CCCAGGTGCAGGCGAGCGCCCTGTCTCCCACCCCCAGTGAGTGCCCAGAGAGTCAGAAGGATGGTAATGG
  GCCCGAAGACCCTAAATCACAGGTGGGGCCTGAGGACCCCAAGTCACAAGTGGGGCCTGAGGATCCTAAA
  AGCCAAGTCGGGCCTGAAGACCCCAAGTCCCAGGTCGGACCTGAGGACCCCAAGGGTCAGGTAGAACCAG
  AAGACCCCAAGGGCCAGGTGGGACCAGAAGACCCTAAGGGTCAAGTGGGACCTGAGGACCCCAAGGGACA
  GGTGGGCCCCGAGGACCCTAAGTCACAGGTAGGGCCTGAAGATCCAAAAAGCCAAGTGGAACCAGAGGAT
  CCAAAGAGCCAGGTCGAACCTGAGGACCCAAAGAGTCAGGTCGAACCCGAGGACCCCAAGTCCCAAGTGG
  GCCCAGAGGACCCCCAGAGCCAGGTCGGACCCGAGCAGGCGGCCTCTAAGAGCCTGGGCGAGGACCCAGA
  CAGTGATACAACGGGTACATCCATGAGCGAATCCGAAGAGCTGGCTCGCATTGAGGCATCTGTCGAACCA
  CCAAAACCA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG209623 representing NM_031373
  Red=Cloning site Green=Tags(s)
  
  MDDPECDSTWEDESEEDGEDGQADDTTDEDTGDDDGDAEEARPSLFQSRMTRYRNWRAMQDMQRYRHNYP
  DLTDQDCNGDMCNLSFYKNEICFQPNGFLIEDILQNWKDNYDLLEENHSYIQWLFPLREPGVNWHAKPLT
  LKEVEAFKSSKEVRERLVRAYELMLGFYGIQLEDRNTGAVCRAQNFQPRFHNLNSHSHNNLRITRILKSL
  GELGLEHYQAPLVRFFLEETLVQHKLPSVRQSALDYFLFAVRCRHQRRELVHFAWEHFKPRREFVWGPRD
  KLRRFRPQTISRPLMGLGQADKDEGPGDPSQEAGTQGRTCGSGRDLSGDSGTAEDLSLLSAKPQDVGTLD
  GDQRHEAKSPSPKESKKRKLEGNRQEQVPGEPDPQGVSEVEKIALNLEGCALSPTSQEPREAEQPCLVAR
  VANEVRKRRKVEEGAEGDGVASNTQVQASALSPTPSECPESQKDGNGPEDPKSQVGPEDPKSQVGPEDPK
  SQVGPEDPKSQVGPEDPKGQVEPEDPKGQVGPEDPKGQVGPEDPKGQVGPEDPKSQVGPEDPKSQVEPED
  PKSQVEPEDPKSQVEPEDPKSQVGPEDPQSQVGPEQAASKSLGEDPDSDTTGTSMSESEELARIEASVEP
  PKP
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_031373
• ORF Size: 1899 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_031373.3, NP_113550.3
• RefSeq Size: 2306 bp
• RefSeq ORF: 1902 bp
• Locus ID: 72075
• UniProt ID: Q99PG2
• Cytogenetics: 2 H4
• Gene Summary: Receptor for opioid growth factor (OGF), also known as Met-enkephalin. Seems to be involved in growth regulation (By similarity).[UniProtKB/Swiss-Prot Function]