Vps52 (BC063329) Mouse Tagged ORF Clone

CAT#: MG209491

Vps52 (tGFP-tagged) - Mouse vacuolar protein sorting 52 (yeast) (cDNA clone MGC:74357 IMAGE:30251096)

Product Images

Circular map for MG209491

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Vps52
• Synonyms: D130068D18, ARE1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG209491 representing BC063329
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGCCGCAGCGACCATGGCGGCCGCTGCCCGGGAGCTGGTGTTGCGGGCTGGGGCCTCAGATGTGG
  AGGAGGAGGAGGGCCCGCTGGGGGGGGGTTCTGGACTCCAAGAACCCCTACAACTTGGAGAGTTGGACAT
  CACCTCTGATGAATTCATCTTGGATGAAGTGGATGTTCACATCCAGGCAAACCTGGAGGATGAGTTAGTG
  AAGGAAGCTCTCAAAACGGGTGTGGATCTTCGACACTATTCAAAGCAGGTGGAGCTGGAGCTGCAGCAGA
  TTGAGCAGAAGTCAATCCGGGACTATATCCAAGAGAGTGAGAACATAGCGTCTCTGCACAATCAGATCAC
  GGCCTGTGACGCCGTCCTGGAGCCCAGGTTCCTGGAGCAGCTGCAGGAGCTGGATGCCAAGGCAGCCGCG
  GTGAGAGAGCAGGAGGCTATGGGCACCGCTGCCTGTGCTGACGTCAGAGGAGTGCTGGACCGGCTCCGGG
  TCAAGGCAGTGACGAAGATCCGGGAGTTCATTCTCCAGAAGATCTACTCCTTCAGAAAGCCCATGACCAA
  CTACCAGATCCCCCAGGCGGCCCTGCTGAAGTACAGGTTTTTCTATCAGTTCCTGCTGGGCAATGAGCGT
  GCTACAGCCAAAGAGATCAGGGATGAGTACGTAGAGACGCTGAGCAAGATCTACCTGTCCTACTACCGAT
  CCTATGTGGGGCGGCTCATGAAAGTGCAGTACGAGGAAGTTGCTGAGAAAGACGACCTAATGGGTGTTGA
  AGACACAGCAAAGAAAGGCTTCTTCTCGAAGCCGTCCCTCCGAAGCAGGAACACCATCTTTACCCTTGGC
  ACTCGTGGTACTGTCATCTCACCGGCCGAGCTGGAGGCCCCCATCCTAGTGCCCCATACTGCCCAACGTG
  GAGAGCAGAGGTATCCATTCGAAGCGCTCTTCCGCAGCCAGCACTATGCCCTCCTCGACAATTCTTGCCG
  TGAATATCTTTTCATCTGTGAATTTTTCATCGTGTCTGGCCCGGCTGCACATGACCTGTTCCACGCCGTC
  ATGGGCCGCACGCTCTCCATGACACTGAAACACCTGGAGTCCTACCTGGCTGACTGCTACGACGCCATTG
  CTGTTTTTCTCTGTATCCACATTGTTCTCCGGTTCCGCAACATTGCAGGGAAGAGGGACGTCCCTGCCCT
  GGACAGGTACTGGGAGCAGGTGCTTGCCTTGCTGTGGCCTCGGTTTGAGCTGATCCTGGAGATGAATGTC
  CAGAGTGTCCGCAGCACTGACCCCCAGCGCCTTGGGGGACTGGACACTCGGCCCCACTATATCACACGCC
  GCTATGCTGAGTTCTCCTCTGCACTTGTCAGCATCAACCAGACGATCCCCAATGAACGCACGCTGCAGCT
  CCTGGGACAGCTCCAGGTGGAGGTGGAGAATTTTGTCCTCCGAGTGGCTGCAGAGTTCTCCTCCAGGAAG
  GAGCAGCTTGTGTTTCTGATCAACAACTATGACATGATGCTCGGGGTGCTGATGGAGCGGGCTGCTGATG
  ACAGCAAAGAGGTGGAGAGTTTCCAGCAGCTGCTCAATGCTCGGACACAGGAGTTCATTGAGGAGCTGCT
  GTCTCCCCCCTTCGGGGGTCTGGTGGCATTCGTGAAGGAGGCTGAAGCCTTGATTGAGCGTGGGCAGGCT
  GAGCGGCTCCGAGGGGAGGAAGCCCGAGTCACTCAGCTGATCCGTGGCTTTGGTAGTTCCTGGAAGGCGT
  CAGTGGAGTCCCTGAGTCAGGATGTAATGCGAAGTTTTACCAACTTCCGAAATGGAACCAGCATCATCCA
  GGGGGCACTGACCCAGCTGAAGAAACACAAGCCGAACTTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG209491 representing BC063329
  Red=Cloning site Green=Tags(s)
  
  MAAAATMAAAARELVLRAGASDVEEEEGPLGGGSGLQEPLQLGELDITSDEFILDEVDVHIQANLEDELV
  KEALKTGVDLRHYSKQVELELQQIEQKSIRDYIQESENIASLHNQITACDAVLEPRFLEQLQELDAKAAA
  VREQEAMGTAACADVRGVLDRLRVKAVTKIREFILQKIYSFRKPMTNYQIPQAALLKYRFFYQFLLGNER
  ATAKEIRDEYVETLSKIYLSYYRSYVGRLMKVQYEEVAEKDDLMGVEDTAKKGFFSKPSLRSRNTIFTLG
  TRGTVISPAELEAPILVPHTAQRGEQRYPFEALFRSQHYALLDNSCREYLFICEFFIVSGPAAHDLFHAV
  MGRTLSMTLKHLESYLADCYDAIAVFLCIHIVLRFRNIAGKRDVPALDRYWEQVLALLWPRFELILEMNV
  QSVRSTDPQRLGGLDTRPHYITRRYAEFSSALVSINQTIPNERTLQLLGQLQVEVENFVLRVAAEFSSRK
  EQLVFLINNYDMMLGVLMERAADDSKEVESFQQLLNARTQEFIEELLSPPFGGLVAFVKEAEALIERGQA
  ERLRGEEARVTQLIRGFGSSWKASVESLSQDVMRSFTNFRNGTSIIQGALTQLKKHKPNF
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC063329
• ORF Size: 1860 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC063329.1
• RefSeq Size: 2460 bp
• RefSeq ORF: 1862 bp
• Locus ID: 224705
• Cytogenetics: 17 B1
• Gene Summary: Acts as component of the GARP complex that is involved in retrograde transport from early and late endosomes to the trans-Golgi network (TGN). The GARP complex is required for the maintenance of the cycling of mannose 6-phosphate receptors between the TGN and endosomes, this cycling is necessary for proper lysosomal sorting of acid hydrolases such as CTSD. Acts as component of the EARP complex that is involved in endocytic recycling. The EARP complex associates with Rab4-positive endosomes and promotes recycling of internalized transferrin receptor (TFRC) to the plasma membrane.[UniProtKB/Swiss-Prot Function]