Slc17a8 (NM_182959) Mouse Tagged ORF Clone

CAT#: MG209312

Slc17a8 (tGFP-tagged) - Mouse solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter), member 8 (Slc17a8)

Product Images

Circular map for MG209312

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Slc17a8
• Synonyms: BC042593; Vglut3
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG209312 representing NM_182959
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCATTTAAAGCATTTGATACCTTCAAAGAAAAGATTTTGAAACCTGGGAAGGAAGGAGTGAAGAATG
  CCGTGGGAGACTCGCTGGGCATCTTACAAAGAAAAATCGATGGGACCAATGAAGAGGAAGATGCCATTGA
  GCTGAATGAAGAAGGAAGGCCCGTGCAGACGTCCAGAGCACATCGCCCTGTCTGTGACTGCAGCTGCTGC
  GGCATCCCCAAGCGGTACATCTGTGACTGCAGCTGCTGCGGCATCCCCAAACGGTACATCATTGCTGTCA
  TGAGCGGCCTCGGCTTCTGCATTTCCTTTGGGATTCGGTGCAACCTTGGAGTGGCCATTGTGGAAATGGT
  CAACAATAGCACTGTGTATGTGGATGGGAAACCAGAAATTCAGACAGCGCAGTTTAACTGGGACCCAGAG
  ACGGTGGGCCTGATCCATGGATCTTTTTTCTGGGGTTATATTGTGACACAAATTCCCGGTGGCTTCATTT
  CAAACAAGTTTGCTGCTAGCAGGGTCTTTGGAGCCGCCATCTTCTTGACATCAACTCTGAACATGTTTAT
  CCCTTCTGCGGCGAGGGTGCATTACGGCTGTGTCATGGGTGTGAGGATTTTGCAGGGTCTGGTGGAGGGT
  GTGACCTACCCTGCCTGCCATGGGATGTGGAGTAAGTGGGCGCCTCCCCTGGAGAGAAGCCGTCTAGCCA
  CGACTTCCTTCTGTGGTTCCTATGCTGGGGCAGTTGTTGCTATGCCTCTTGCAGGGGTATTGGTGCAGTA
  CATTGGCTGGGCCTCTGTCTTTTACATCTACGGTATGTTTGGGATTATTTGGTATATGTTTTGGCTGCTG
  CAGGCTTATGAGTGCCCGGCAGCTCACCCAACAATATCCAATGCAGAAAGGACCTACATAGAGACAAGCA
  TAGGAGAAGGGGCCAACTTGGCCAGTCTGAGCAAATTCAACACACCATGGAGAAGGTTTTTCACATCCTT
  GCCTGTCTATGCCATCATTGTGGCAAACTTCTGTAGAAGCTGGACCTTCTATTTGCTCCTGATAAGTCAG
  CCAGCTTACTTTGAGGAGGTCTTTGGATTTGCAATAAGTAAGGTGGGTCTCTTGTCAGCTGTCCCACACA
  TGGTGATGACAATCGTGGTGCCCATCGGAGGACAACTGGCTGATTATTTAAGAAGCCGAAAGATTTTGAC
  CACAACCGCTGTCAGAAAGATCATGAATTGTGGAGGCTTTGGCATGGAGGCAACCTTGCTCCTGGTGGTT
  GGATTTTCCCATACCAAAGGAGTGGCTATCTCCTTCCTGGTGCTTGCTGTAGGATTTAGTGGCTTTGCTA
  TTTCAGGCTTCAATGTCAACCACCTGGACATTGCTCCACGCTATGCCAGCATCCTCATGGGGATCTCAAA
  TGGTGTGGGGACCCTCTCTGGAATGGTTTGTCCCCTCATTGTTGGTGCAATGACTAAGCACAAGACCCGG
  GAGGAGTGGCAGAATGTGTTCCTCATAGCAGCGCTGGTACACTACAGCGGAGTCATCTTCTACGGGGTCT
  TCGCTTCTGGGGAAAAACAGGACTGGGCTGACCCAGAGAATCTCTCTGAGGACAAATGTGGAATCATTGA
  CCAAGATGAGTTAGCTGAGGAGACAGAACTCAACCACGAGACTTTCGTAAGTCCCAGAAAGAAGATGTCT
  TATGGAGCCACCACCCAGAATTGTGAGGTCCAGAAGACGGAGTGGAGACAACAGAGAGAATCTGCCTTCG
  ACGGGGAGGAGCCATTATCCTACCAGGCCGAAGGAGACTTCTCAGAAACATCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG209312 representing NM_182959
  Red=Cloning site Green=Tags(s)
  
  MPFKAFDTFKEKILKPGKEGVKNAVGDSLGILQRKIDGTNEEEDAIELNEEGRPVQTSRAHRPVCDCSCC
  GIPKRYICDCSCCGIPKRYIIAVMSGLGFCISFGIRCNLGVAIVEMVNNSTVYVDGKPEIQTAQFNWDPE
  TVGLIHGSFFWGYIVTQIPGGFISNKFAASRVFGAAIFLTSTLNMFIPSAARVHYGCVMGVRILQGLVEG
  VTYPACHGMWSKWAPPLERSRLATTSFCGSYAGAVVAMPLAGVLVQYIGWASVFYIYGMFGIIWYMFWLL
  QAYECPAAHPTISNAERTYIETSIGEGANLASLSKFNTPWRRFFTSLPVYAIIVANFCRSWTFYLLLISQ
  PAYFEEVFGFAISKVGLLSAVPHMVMTIVVPIGGQLADYLRSRKILTTTAVRKIMNCGGFGMEATLLLVV
  GFSHTKGVAISFLVLAVGFSGFAISGFNVNHLDIAPRYASILMGISNGVGTLSGMVCPLIVGAMTKHKTR
  EEWQNVFLIAALVHYSGVIFYGVFASGEKQDWADPENLSEDKCGIIDQDELAEETELNHETFVSPRKKMS
  YGATTQNCEVQKTEWRQQRESAFDGEEPLSYQAEGDFSETS
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_182959
• ORF Size: 1803 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_182959.3
• RefSeq Size: 4481 bp
• RefSeq ORF: 1806 bp
• Locus ID: 216227
• UniProt ID: Q8BFU8
• Cytogenetics: 10 C2
• Gene Summary: Mediates the uptake of glutamate into synaptic vesicles at presynaptic nerve terminals of excitatory neural cells. May also mediate the transport of inorganic phosphate.[UniProtKB/Swiss-Prot Function]