Eps15l1 (BC015259) Mouse Tagged ORF Clone

CAT#: MG209295

Eps15l1 (tGFP-tagged) - Mouse epidermal growth factor receptor pathway substrate 15-like 1 (cDNA clone MGC:18416 IMAGE:4238768)

Product Images

Circular map for MG209295

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Eps15l1
• Synonyms: 9830147J04Rik; AI593686; Eps15-rs; Eps15R
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG209295 representing BC015259
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCGCCGCTTGTACCCCTCTCGCAGCAGATTCCCAGTGGAAATCCGTTATACGAGTCTTATTACA
  AGCAGGTGGACCCTGCCTACACAGGGAGAGTTGGGGCAAGCGAGGCTGCGCTGTTTCTGAAGAAATCGGG
  GCTCTCAGACATCATCCTGGGGAAGATATGGGACTTGGCAGACCCCGAAGGTAAAGGGTTCTTGGACAAA
  CAGGGTTTCTATGTTGCACTGCGACTGGTGGCCTGTGCACAGAGTGGCCATGAAGTCACCTTGAGCAGTC
  TGAGCCTCACCATGCCACCACCAAAATTTCATGATACCAGCAGCCCACTGATGGCCACACAGTCATCTGC
  AGAGACCCACTGGGCTGTGCGGGTGGAGGAGAAGGCAAAGTTTGATGGCATCTTTGAAAGCCTTCTACCA
  GTCAATGGGCTACTCTCTGGAGACAAGGTCAAGCCGGTCCTCATGAACTCGAAACTACCTCTGGATGTGC
  TGGGCAGGGTCTGGGACCTCAGTGACATAGACAAGGATGGACATTTGGACCGAGATGAGTTTGCTGTGGC
  TATGCACTTGGTGTACCGCGCCCTGGAGAAGGAACCAGTGCCCTCCATCCTGCCCCCGCCCCTCATCCCA
  CCCTCTAAGAGGAAGAAGACTGTGTTTGCAGGAGCTGTGCCTGTCCTGCCTGCTAGTCCCCCACCCAAAG
  ACAGCCTCCGCTCCACACCATCCCACGGCAGCGTAAGCAGCCTCAACAGCACAGGCAGCCTGTCCCCGAA
  GCACAGCGTCAAGCAGCCACCAGTGGCCTGGGTTGTGCCTGTGGCAGATAAGATGCGCTTTGATGAGATC
  TTTTTGAAGACAGACCTGGACCTTGATGGCTATGTGAGTGGCCAGGAAGTTAAGGAGATCTTCATGCACT
  CAGGCCTCACTCAGAACCTTCTGGCACACATCTGGGCCCTGGCTGATACAAGACAAACGGGCAAGTTAAG
  CAAAGAGCAGTTCGCACTAGCTATGTATTTCATTCAGCAGAAGGTCAGTAAAGGCATCGACCCTCCTCAA
  GTCCTCTCGCCTGACATGGTGCCACCCTCTGAGAGAGGCACTCCCATTCCAGACAGTTCAAGTACTCTGG
  CATCAGGAGAGTTCACTGGTGTTAAAGAGCTGGATGACATTAGCCAGGAGATCGCACAGTTGCAGAGAGA
  GAAATACTCATTGGAACAAGACATCAGAGAAAAGGAAGAGGCAATCAAACAGAAAACCAGCGAAGTACAG
  GAATTACAAAATGACCTAGACCGGGAAACTAGTAGTCTGCAGGAGCTTGAGGCTCAGAAACAGGATGCCC
  AGGACCGCCTGGATGAGATGGACCAGCAGAAGGCTAAGCTGCGGGACATGCTGAGTGACGTGCGGCAGAA
  ATGCCAGGATGAGACCCAGACAATCTCATCATTGAAAACCCAGATTCAGTCTCAGGAGTCAGACTTGAAG
  TCCCAGGAGGATGACCTGAACAGGGCCAAGTCAGAGCTGAATCGGCTGCAGCAAGAGGAGACACAGCTGG
  AGCAGAGCATCCAGGCTGGACGTGCACAGCTGGAGACCATCCTCAGGTCCCTGAAGTGCACACAGGATGA
  CATCAACCAGGCAAGAAGCAAGCTGTCCCAGCTACAGGAGAGCCACCTAGAAGCTCACCGGAGCCTGGAA
  CAGTATGACCAGGTGCCTGATGGGGTCTCTGGTACCAGCCTGCCTGACCTGGCCACCTTGAACGAAGGCA
  TCTTGCTGGCAGAGAGGGGCGGCTTTGGAGCTATGGTAAAGGTCGAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG209295 representing BC015259
  Red=Cloning site Green=Tags(s)
  
  MAAPLVPLSQQIPSGNPLYESYYKQVDPAYTGRVGASEAALFLKKSGLSDIILGKIWDLADPEGKGFLDK
  QGFYVALRLVACAQSGHEVTLSSLSLTMPPPKFHDTSSPLMATQSSAETHWAVRVEEKAKFDGIFESLLP
  VNGLLSGDKVKPVLMNSKLPLDVLGRVWDLSDIDKDGHLDRDEFAVAMHLVYRALEKEPVPSILPPPLIP
  PSKRKKTVFAGAVPVLPASPPPKDSLRSTPSHGSVSSLNSTGSLSPKHSVKQPPVAWVVPVADKMRFDEI
  FLKTDLDLDGYVSGQEVKEIFMHSGLTQNLLAHIWALADTRQTGKLSKEQFALAMYFIQQKVSKGIDPPQ
  VLSPDMVPPSERGTPIPDSSSTLASGEFTGVKELDDISQEIAQLQREKYSLEQDIREKEEAIKQKTSEVQ
  ELQNDLDRETSSLQELEAQKQDAQDRLDEMDQQKAKLRDMLSDVRQKCQDETQTISSLKTQIQSQESDLK
  SQEDDLNRAKSELNRLQQEETQLEQSIQAGRAQLETILRSLKCTQDDINQARSKLSQLQESHLEAHRSLE
  QYDQVPDGVSGTSLPDLATLNEGILLAERGGFGAMVKVE
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC015259
• ORF Size: 1799 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC015259, AAH15259
• RefSeq Size: 3404 bp
• RefSeq ORF: 1799 bp
• Locus ID: 13859
• Cytogenetics: 8 B3.3
• Gene Summary: Seems to be a constitutive component of clathrin-coated pits that is required for receptor-mediated endocytosis. Involved in endocytosis of integrin beta-1 (ITGB1) and transferrin receptor (TFR); internalization of ITGB1 as DAB2-dependent cargo but not TFR seems to require association with DAB2 (By similarity).[UniProtKB/Swiss-Prot Function]