Carm1 (BC036974) Mouse Tagged ORF Clone

CAT#: MG209145

Carm1 (tGFP-tagged) - Mouse coactivator-associated arginine methyltransferase 1 (cDNA clone MGC:46828 IMAGE:4935077)

Product Images

Circular map for MG209145

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Carm1
• Synonyms: MGC46828
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG209145 representing BC036974
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGCGGCGGCAGCGACGGCGGTGGGGCCGGGTGCGGGGAGCGCTGGGGTGGCGGGCCCGGGCGGCG
  CGGGGCCCTGCGCTACAGTGTCTGTGTTCCCGGGCGCCCGCCTCCTCACTATCGGCGACGCGAACGGCGA
  GATCCAGCGGCACGCGGAGCAGCAGGCGCTGCGCCTTGAGGTGCGCGCCGGACCAGACGCGGCGGGCATC
  GCCCTCTACAGCCATGAAGATGTGTGTGTTTTCAAGTGCTCGGTGTCCCGAGAGACAGAGTGCAGTCGTG
  TGGGCAGACAGTCCTTCATCATCACCCTGGGCTGCAACAGCGTCCTCATCCAGTTTGCCACACCCCACGA
  TTTCTGTTCTTTCTACAACATCCTGAAAACCTGTCGGGGCCACACACTGGAGCGCTCTGTGTTCAGTGAG
  CGGACAGAGGAATCCTCAGCTGTGCAGTACTTCCAGTTCTATGGCTACCTATCCCAGCAGCAGAACATGA
  TGCAGGACTATGTGCGGACAGGCACCTACCAGCGTGCGATCCTGCAGAACCACACGGACTTCAAGGACAA
  GATCGTTCTAGATGTGGGCTGTGGCTCTGGGATCCTGTCATTTTTTGCTGCTCAAGCAGGAGCCAGGAAA
  ATTTATGCAGTGGAAGCCAGCACCATGGCTCAGCATGCAGAGGTCCTGGTGAAGAGTAACAATCTGACAG
  ACCGCATCGTGGTCATCCCTGGCAAAGTAGAGGAGGTCTCATTGCCTGAGCAAGTGGACATTATCATCTC
  AGAGCCCATGGGCTACATGCTCTTCAATGAACGAATGCTCGAGAGCTACCTCCATGCCAAAAAGTACCTG
  AAGCCTAGTGGAAACATGTTCCCCACCATTGGTGATGTCCACCTCGCACCCTTCACTGATGAACAGCTCT
  ACATGGAGCAGTTCACCAAAGCCAACTTCTGGTACCAGCCATCCTTCCATGGAGTGGACCTGTCGGCCCT
  CAGAGGTGCCGCTGTGGATGAGTACTTCCGGCAACCTGTGGTGGACACATTTGACATCCGGATCCTGATG
  GCCAAATCTGTCAAGTACACAGTGAACTTCTTAGAAGCCAAAGAAGGCGATTTGCACAGGATAGAAATCC
  CATTCAAATTCCACATGCTGCATTCAGGGCTAGTCCATGGCTTGGCCTTCTGGTTCGATGTTGCTTTCAT
  TGGCTCCATAATGACCGTGTGGCTATCCACAGCCCCAACAGAGCCCCTGACCCACTGGTACCAGGTCCGG
  TGCCTCTTCCAGTCACCGTTGTTTGCCAAGGCCGGGGACACGCTCTCAGGGACATGTCTGCTTATTGCCA
  ACAAAAGACAGAGCTATGACATCAGTATTGTGGCACAGGTGGACCAGACAGGCTCCAAGTCCAGTAACCT
  GCTGGATCTAAAGAACCCCTTCTTCAGGTACACAGGTACAACCCCATCACCCCCACCTGGCTCACACTAC
  ACGTCTCCCTCGGAGAATATGTGGAACACAGGAAGCACCTATAATCTCAGCAGCGGGGTGGCTGTGGCTG
  GAATGCCTACTGCCTACGACCTGAGCAGTGTTATTGCCGGCGGCTCCAGTGTGGGTCACAACAACCTGAT
  TCCCTTAGGCTCCTCAGGTGCCCAGGGAGGCGGCGGTAGCTCCAGTGCCCACTATGCAGTCAACAACCAG
  TTCACCATGGGTGGCCCTGCCATCTCTATGGCCTCGCCCATGTCCATCCCGACCAACACCATGCACTATG
  GGAGT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG209145 representing BC036974
  Red=Cloning site Green=Tags(s)
  
  MAAAAATAVGPGAGSAGVAGPGGAGPCATVSVFPGARLLTIGDANGEIQRHAEQQALRLEVRAGPDAAGI
  ALYSHEDVCVFKCSVSRETECSRVGRQSFIITLGCNSVLIQFATPHDFCSFYNILKTCRGHTLERSVFSE
  RTEESSAVQYFQFYGYLSQQQNMMQDYVRTGTYQRAILQNHTDFKDKIVLDVGCGSGILSFFAAQAGARK
  IYAVEASTMAQHAEVLVKSNNLTDRIVVIPGKVEEVSLPEQVDIIISEPMGYMLFNERMLESYLHAKKYL
  KPSGNMFPTIGDVHLAPFTDEQLYMEQFTKANFWYQPSFHGVDLSALRGAAVDEYFRQPVVDTFDIRILM
  AKSVKYTVNFLEAKEGDLHRIEIPFKFHMLHSGLVHGLAFWFDVAFIGSIMTVWLSTAPTEPLTHWYQVR
  CLFQSPLFAKAGDTLSGTCLLIANKRQSYDISIVAQVDQTGSKSSNLLDLKNPFFRYTGTTPSPPPGSHY
  TSPSENMWNTGSTYNLSSGVAVAGMPTAYDLSSVIAGGSSVGHNNLIPLGSSGAQGGGGSSSAHYAVNNQ
  FTMGGPAISMASPMSIPTNTMHYGS
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC036974
• ORF Size: 1757 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC036974, AAH36974
• RefSeq Size: 3171 bp
• RefSeq ORF: 1757 bp
• Locus ID: 59035
• Cytogenetics: 9 A3
• Gene Summary: Methylates (mono- and asymmetric dimethylation) the guanidino nitrogens of arginyl residues in several proteins involved in DNA packaging, transcription regulation, pre-mRNA splicing, and mRNA stability. Recruited to promoters upon gene activation together with histone acetyltransferases from EP300/P300 and p160 families, methylates histone H3 at 'Arg-17' (H3R17me), forming mainly asymmetric dimethylarginine (H3R17me2a), leading to activates transcription via chromatin remodeling. During nuclear hormone receptor activation and TCF7L2/TCF4 activation, acts synergically with EP300/P300 and either one of the p160 histone acetyltransferases NCOA1/SRC1, NCOA2/GRIP1 and NCOA3/ACTR or CTNNB1/beta-catenin to activate transcription. During myogenic transcriptional activation, acts together with NCOA3/ACTR as a coactivator for MEF2C. During monocyte inflammatory stimulation, acts together with EP300/P300 as a coactivator for NF-kappa-B. Acts as coactivator for PPARG, promotes adipocyte differentiation and the accumulation of brown fat tissue. Plays a role in the regulation of pre-mRNA alternative splicing by methylation of splicing factors. Also seems to be involved in p53/TP53 transcriptional activation. Methylates EP300/P300, both at 'Arg-2142', which may loosen its interaction with NCOA2/GRIP1, and at 'Arg-580' and 'Arg-604' in the KIX domain, which impairs its interaction with CREB and inhibits CREB-dependent transcriptional activation. Also methylates arginine residues in RNA-binding proteins PABPC1, ELAVL1 and ELAV4, which may affect their mRNA-stabilizing properties and the half-life of their target mRNAs.[UniProtKB/Swiss-Prot Function]