Appbp2 (NM_025825) Mouse Tagged ORF Clone

CAT#: MG209142

Appbp2 (tGFP-tagged) - Mouse amyloid beta precursor protein (cytoplasmic tail) binding protein 2 (Appbp2)

Product Images

Circular map for MG209142

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Appbp2
• Synonyms: 1300003O07Rik; AI465480; PAT1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG209142 representing NM_025825
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCCGTGGAGCTCGAGTGGATCCCGGAGACGCTCTACAACACCGCCATCTCCGCTGTCGTGGACA
  ACTACATCCGCTCGCGCCGCGACATCCGCTCCTTGCCGGAGAATATCCAGTTCGACGTTTACTACAAGCT
  TTACCAACAGGGACGCTTGTGTCAGCTCGGCAGTGAATTCTGTGAATTGGAAGTTTTTGCTAAAGTTTTG
  AGAGCTTTGGATAAAAGACATTTGCTCCACCACTGTTTTCAGGCTCTGATGGACCATGGCGTTAAGGTAG
  CTTCCGTCTTGGCCTACTCCTTCAGTAGACGGTGCTCTTACATAGCAGAGTCCGACGCTGCAGTAAAGGA
  GAAGGCCATCCAGGTTGGCTTTGTCTTAGGTGGCTTTCTTTCTGATGCAGGCTGGTACAGTGATGCTGAA
  AAGGTTTTTCTGTCCTGCCTTCAGTTGTGTACGCTACATGATGAGATGCTCCACTGGTTCCGAGCAGTGG
  AGTGTTGTGTGAGGTTGCTTCATGTGCGAAATGGAAATTGTAAATATCATCTAGGTGAAGAAACATTTAA
  ATTAGCTCAGACATACATGGATAAGCTGTCTAAACATGGCCAGCAAGCAAATAGAGCTGCTCTCTACGGA
  GAACTATGTGCACTCCTGTTTGCAAAAAGCCACTATGATGAGGCATACAAGTGGTGCGTTGAGGCAATGA
  AGGAAATTACAGCAGGCTTACCAGTCAAAGTTGTGGTGGATGTTTTAAGACAAGCTTCTAAGGCTTGTGT
  AGTCAAACGTGAATTTAAGAAGGCAGAACAGTTAATTAAACATGCAGTGTATTTGGCACGAGATCATTTC
  GGATCCAAACACCCAAAATACTCTGACACACTGCTAGATTATGGGTTCTACTTACTCAATGTAGACAATA
  TCTGTCAGTCTGTTGCAATTTACCAGGCAGCCCTCGACATTCGACAGTCAGTGTTTGGTGGCAAAAATAT
  TCATGTAGCAACAGCTCATGAAGACCTGGCCTACTCTTCCTACGTTCACCAGTACAGCTCTGGAAAATTC
  GACAATGCACTATTTCATGCAGAAAGAGCCATTGGTATCATTACTCACATCCTACCTGAAGATCATCTTC
  TTCTGGCTTCTTCAAAGAGGGTGAAAGCACTTATTCTAGAGGAGATTGCGATTGACTGTCACAACAAGGA
  GACGGAGCAGCGGCTGCTTCAGGAGGCACACGACTTGCACTTGTCTTCGCTGCAGCTGGCTAAGAAAGCA
  TTTGGGGAATTTAATGTACAGACTGCAAAGCACTATGGGAACCTCGGAAGGCTCTATCAGTCAATGAGAA
  AATTTAAGGAAGCTGAAGAAATGCACATAAAAGCAATTCAGATTAAGGAGCAGCTCCTTGGCCAAGAAGA
  TTATGAAGTGGCCCTTTCAGTGGGACACCTGGCATCTCTGTATAATTATGACATGAATCAGTATGAAAAT
  GCTGAGAAACTGTACCTGCGGTCGATAGCCATTGGGAAGAAACTGTTTGGTGAAGGCTACAGTGGACTAG
  AATATGATTACCGGGGCCTCATTAAACTTTACAACTCCATTGGGAATTATGAGAAAGTGTTCGAGTATCA
  CAACGTTCTGTCTAACTGGAACCGGTTGCGAGATCGACAGTATTCAGTGACCGATGCCCTTGAAGACGTC
  AGCAGCAGCCCCCAGTCCACTGAAGAAGTGGTGCAGTCCTTTCTGATGGCTCAGAATGTTGAGGGACCAA
  GCTGC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG209142 representing NM_025825
  Red=Cloning site Green=Tags(s)
  
  MAAVELEWIPETLYNTAISAVVDNYIRSRRDIRSLPENIQFDVYYKLYQQGRLCQLGSEFCELEVFAKVL
  RALDKRHLLHHCFQALMDHGVKVASVLAYSFSRRCSYIAESDAAVKEKAIQVGFVLGGFLSDAGWYSDAE
  KVFLSCLQLCTLHDEMLHWFRAVECCVRLLHVRNGNCKYHLGEETFKLAQTYMDKLSKHGQQANRAALYG
  ELCALLFAKSHYDEAYKWCVEAMKEITAGLPVKVVVDVLRQASKACVVKREFKKAEQLIKHAVYLARDHF
  GSKHPKYSDTLLDYGFYLLNVDNICQSVAIYQAALDIRQSVFGGKNIHVATAHEDLAYSSYVHQYSSGKF
  DNALFHAERAIGIITHILPEDHLLLASSKRVKALILEEIAIDCHNKETEQRLLQEAHDLHLSSLQLAKKA
  FGEFNVQTAKHYGNLGRLYQSMRKFKEAEEMHIKAIQIKEQLLGQEDYEVALSVGHLASLYNYDMNQYEN
  AEKLYLRSIAIGKKLFGEGYSGLEYDYRGLIKLYNSIGNYEKVFEYHNVLSNWNRLRDRQYSVTDALEDV
  SSSPQSTEEVVQSFLMAQNVEGPSC
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_025825
• ORF Size: 1755 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_025825.3, NP_080101.1
• RefSeq Size: 2407 bp
• RefSeq ORF: 1758 bp
• Locus ID: 66884
• UniProt ID: Q9DAX9
• Cytogenetics: 11 C
• Gene Summary: May play a role in intracellular protein transport. May be involved in the translocation of APP along microtubules toward the cell surface (By similarity).[UniProtKB/Swiss-Prot Function]