Klhl26 (NM_172052) Mouse Tagged ORF Clone

CAT#: MG209001

Klhl26 (tGFP-tagged) - Mouse kelch-like 26 (Drosophila) (Klhl26), transcript variant 1

Product Images

Circular map for MG209001

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Klhl26
• Synonyms: Klkl26, MGC38024, C630013N10Rik
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG209001 representing NM_172052
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGAGTCCGGTGGCAGCAGCGGCTCTAGTCAGAGCCCCGAGCGCCCGAGCAGCTTGGCCGACAGGA
  ATGGGGCACTTAAGTGCACCTTCTCTGCAGCTGGCCACAGCACTGGCCTCCTGCAGGGCCTGGCTGCCCT
  CCGTGCACAGGGCCAGCTGCTCGATGTGGTCCTCACTGTCAACAGTGAAGCCTTTCATGCGCACAAGGTG
  GTCTTGGCGGCCTGCAGTGACTACTTTAGTGCTGAGGTGACACTGGACCTAGACTGTGTGCAGGACGTGC
  TGGGTGCTGCCGTGTTTCTGCAGATGCTGCCGGTGGTGGAGCTGTGCGAGGACTTCCTGAAAGCTGCCAT
  GAGCGTAGAGACGTGCCTGCACATTGGCCAGATGGCTACCACCTTCAGCCTGACATCACTGCGTGAGTCA
  GTAGATGCCTTCACATTCCGCCACTTCCTGCAGATAGCAGCAGAAGAGGACTTTCTGCGCCTGCCACTCG
  AGCGCCTTGTCTTCTTTCTGCAGAGCAACCGGCTGCAGAGCTGCGCCGAGATTGACCTGTTCCGCGCTGC
  TGTGCGCTGGCTGCAGCATGATCCTGCCCGCCGCGCCCGTGCCAGCCTTGTGCTACGCCACGTGCGCTTC
  CCGCTCATGCAACCTGCTGAACTGGTGGACAGCGTGCAGACACTCGACGTCATGCTCGACGATGCCCTGT
  GTCGCCAGTACCTGCTGGAGGCCTTCAGCTACCACGTGCTGCCCTGCCGCCAGCATGACATGCAGTCGCC
  TCGCACTGCTGTGCGCTCTGATGTAGCGTCCCTGGTGGCCTTCGGGGGCACACCTTATACCGACAGCGAC
  CGAGCCGTCAGCAGCAAGGTGTTCCAGTTACCTGAGCCAGGAGCACGCCACTTCCGCGAGCTCACGGAGA
  TGGAACTGGGCTGTAGCCACGCGGGCGTGGCTGTGCTGGATAACTTCGTGTACGTGGCAGGCGGCCAGCA
  TCTGCAGCACCGCAGTGGCGAAGGCGCTGTGGACGCCTGCTACCGCTATGATCCACAGCGCAATCGCTGG
  CTGCGGCTGCGAGCACTGCGTGAGAGCCGTGTGCAGTTCCAGCTCACTGCACTGGGAGGGCTGTTGTATG
  CCACCGGTGGCCGAAATCGCGCGGGCAGCCTGGCGTCTGTGGAGCGCTACTGTCCACGCCGTGACAGCTG
  GGATTTTGCCTGCCCACTCAAGCGCCGTACCTGGGGCCATGCGGGTGCAGCAGCTGCTGGTCGCCTCTAC
  ATCTCAGGTGGTTATGGCGTCTCAGCGGAGGACAAGAAGGCGCTGCAGTGCTATGATCCAGCAGCCGACC
  GCTGGGAGCCCAGGGCGCCCATGCGTGAGCCCCGCGTGCTGCACGCAATGTTGGGCGCTGCTGGCCGCAT
  CTATGCCCTAGGTGGCCGCATGGACCACGTGGACCGCTGCTTCGATGTGCTGGCTGTGGAGTACTACGTG
  CCGGATGCCGACCAGTGGACCAGTGTGACACCCATGCGAGCTGGTCAGTCGGAAGCTGGCTGCTGTCTAC
  TAGAGAGGAAGATCTACATCGTGGGTGGCTACAACTGGAGGCTGAACAATGTCACAGGCATCGTTCAGGT
  GTACAACACTGAGACGGATGAGTGGGAGAGAGACCTGCACTTCCCCGAGTCCTTTGCAGGCATTGCCTGT
  GCTGCTGTCCTGCTGCCCCGCTCAGGCCATGGGAGG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG209001 representing NM_172052
  Red=Cloning site Green=Tags(s)
  
  MAESGGSSGSSQSPERPSSLADRNGALKCTFSAAGHSTGLLQGLAALRAQGQLLDVVLTVNSEAFHAHKV
  VLAACSDYFSAEVTLDLDCVQDVLGAAVFLQMLPVVELCEDFLKAAMSVETCLHIGQMATTFSLTSLRES
  VDAFTFRHFLQIAAEEDFLRLPLERLVFFLQSNRLQSCAEIDLFRAAVRWLQHDPARRARASLVLRHVRF
  PLMQPAELVDSVQTLDVMLDDALCRQYLLEAFSYHVLPCRQHDMQSPRTAVRSDVASLVAFGGTPYTDSD
  RAVSSKVFQLPEPGARHFRELTEMELGCSHAGVAVLDNFVYVAGGQHLQHRSGEGAVDACYRYDPQRNRW
  LRLRALRESRVQFQLTALGGLLYATGGRNRAGSLASVERYCPRRDSWDFACPLKRRTWGHAGAAAAGRLY
  ISGGYGVSAEDKKALQCYDPAADRWEPRAPMREPRVLHAMLGAAGRIYALGGRMDHVDRCFDVLAVEYYV
  PDADQWTSVTPMRAGQSEAGCCLLERKIYIVGGYNWRLNNVTGIVQVYNTETDEWERDLHFPESFAGIAC
  AAVLLPRSGHGR
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_172052
• ORF Size: 1716 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_172052.1, NP_742049.1
• RefSeq Size: 3004 bp
• RefSeq ORF: 1718 bp
• Locus ID: 234378
• Cytogenetics: 8 B3.3