Zfp128 (NM_153802) Mouse Tagged ORF Clone

CAT#: MG209000

Zfp128 (tGFP-tagged) - Mouse zinc finger protein 128 (Zfp128)

Product Images

Circular map for MG209000

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Zfp128
• Synonyms: 9630016P15Rik; mZnf8; Znf8
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG209000 representing NM_153802
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACCACCAGGACAAAGCAGCAACAGTGGCAATGGCCTCGCGGCCGCAAGCAACACAACTTCAGGAGC
  CAGTGACTTTCCGAGATGTGGCTGTGGACTTTACCCAAGAGGAGTGGGGGCAGCTGGACCCTACCCAGAG
  GACTCTGTATCGTGATGTGATGCTGGAGACCTTTGGGCACCTTCTCTCTGTGGGTCCTGATCTTCCCAAA
  CCTGCAGTTATCTCCCAGCTGGAACAAGGTGCTGAGCTGTGGGTGGCAGACAGAGGAGGCACCGGGGCCT
  GCCATCCAGGCTGGATTCTTGAACCTGAAGACCACACATTGCTCAAGGACCAGGGCCTCCCAAAAATGGA
  ACCATCCCCTATTACAGAAAAGGATGGGTTTGCAAAGGCTGTTCCTTGTCGTTCTATGATAGGGATAGAT
  CAGGAAAGTGATGGCCAGAGGCAGGCACTCAAGAAGGACCAGAGTAACTTGAATGACCCCAAAGAGATAC
  CACTTCAAAGTCAAAGCCACAAAAGTCTTGGACTTGTGGAAGCCTGTGTTCTTGGCTTAAACACTTATCT
  ATTACCAGATATTTCTGGAAGAGAGTATGGCTGTACTTATGACTCACAAGTTAAAAATTCAGAACATAAT
  CCAAGCTTAGTTAGACAACGGACAGACTCCCCTGCAACACAGTCCTTTGATGACAATGGCAGTCAAAAAG
  CCTTTGATCAAATTATGCCCATTACAGAACTCACAAAAAGTCAGGTGCAAGATAAACCCTATAAGTGTAC
  TGATTGTGGGAAGTCATTTAACCATAACGCACATCTCACAGTGCACAAGAGAATTCATACGGGAGAAAGA
  CCTTACATGTGCAAAGAGTGTGGGAAAGCCTTCAGCCAGAACTCCTCCCTTGTTCAACATGAGCGAATCC
  ACACTGGAGACAAGCCTTATAAGTGTGATGAATGTGGGAAATCTTTTTGCCACAGTACACACCTTACAGT
  CCATAGGAGAATTCACACTGGAGAGAAACCCTATGAGTGTCAGGACTGTGGGAGGTCCTTCAATCAGAAT
  TCATCCCTAGGTCGACACAAGAGGACACACACTGGGGAGAAGCCTTATACCTGCAGTGTTTGTGGGAAAT
  CTTTCTCCCGGACCACTTGCCTCTTCCTGCACCTGCGGACTCACACTGAGGAAAGGCCATACGAGTGTAA
  TCACTGTGGAAAGGGCTTCAGGCACAGTTCCTCCCTGGCCCAGCATCAGCGGAAGCACGCTGGTGAGAAA
  CCCTATGAGTGCCGACAGAGGCTGATCTTTGAGCAGGCGCCGGCTTTAATAAAGTACGAATGGACAGAAC
  CCCTCGGCTGTGACTCACCGTTGAGTCAAGGTGAAAGGACTCAACGGAGTGATAGGCCCTTTAAGTGTAA
  TCAGTGTGGAAAGTGTTTCACTCAGAGTTCTCACCTCATCCGACATCAGCTCACCCACAGCAGAGAGGAA
  GAACCACTACGTGGACGTAGCAGAAGGCAAGAACAGCCGTGTAGACGGGGCTCAAGACTCATTCAGAATA
  CAAACTCAAATTCAAGGGAGCTTCCTGTTGCCCAGCCAAAGGCAGGACAAGCCAGCAGAACCCTGGCCCT
  GTTTGACCTTCGTGAAATTATGCAGGAGCAAAACCCTGTGCATGTTATTGGGGTAGAAGAACCTTCAGTG
  GGCAACTCCATGCTGTTTGACACCAGAGAATCTAGA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG209000 representing NM_153802
  Red=Cloning site Green=Tags(s)
  
  MDHQDKAATVAMASRPQATQLQEPVTFRDVAVDFTQEEWGQLDPTQRTLYRDVMLETFGHLLSVGPDLPK
  PAVISQLEQGAELWVADRGGTGACHPGWILEPEDHTLLKDQGLPKMEPSPITEKDGFAKAVPCRSMIGID
  QESDGQRQALKKDQSNLNDPKEIPLQSQSHKSLGLVEACVLGLNTYLLPDISGREYGCTYDSQVKNSEHN
  PSLVRQRTDSPATQSFDDNGSQKAFDQIMPITELTKSQVQDKPYKCTDCGKSFNHNAHLTVHKRIHTGER
  PYMCKECGKAFSQNSSLVQHERIHTGDKPYKCDECGKSFCHSTHLTVHRRIHTGEKPYECQDCGRSFNQN
  SSLGRHKRTHTGEKPYTCSVCGKSFSRTTCLFLHLRTHTEERPYECNHCGKGFRHSSSLAQHQRKHAGEK
  PYECRQRLIFEQAPALIKYEWTEPLGCDSPLSQGERTQRSDRPFKCNQCGKCFTQSSHLIRHQLTHSREE
  EPLRGRSRRQEQPCRRGSRLIQNTNSNSRELPVAQPKAGQASRTLALFDLREIMQEQNPVHVIGVEEPSV
  GNSMLFDTRESR
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_153802
• ORF Size: 1716 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_153802.3, NP_722497.1
• RefSeq Size: 3850 bp
• RefSeq ORF: 1719 bp
• Locus ID: 243833
• UniProt ID: Q8BGV5
• Cytogenetics: 7 A1
• Gene Summary: Transcriptional repressor. May modulate BMP and TGF-beta signal transduction, through its interaction with SMAD proteins.[UniProtKB/Swiss-Prot Function]