Mtmr2 (BC063050) Mouse Tagged ORF Clone

CAT#: MG208990

Mtmr2 (tGFP-tagged) - Mouse myotubularin related protein 2 (cDNA clone MGC:66658 IMAGE:5716297)

Product Images

Circular map for MG208990

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Mtmr2
• Synonyms: 6030445P13Rik
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG208990 representing BC063050
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGAACCAGCCCTGCTTCCAGGAGAGAACATTAAAGATATGGCCAAAGATGTGACTTACATATGTC
  CATTCACTGGTGCTGTGCGAGGAACGCTGACTGTCACAAGTTACAGGTTATATTTCAAGAGCATGGAGCG
  GGACCCCCCATTTGTTCTAGATGCTTCTCTTGGAGTGATAAGTAGAGTAGAAAAAATTGGTGGTGCATCT
  AGCCGAGGTGAAAACTCCTATGGCCTGGAAACTGTGTGCAAGGATATCAGGAACTTGCGATTTGCTCATA
  AACCGGAGGGGCGAACAAGAAGATCCATATTTGAGAATCTAATGAAATATGCATTTCCTGTGTCTAATGG
  CTTGCCTCTTTTTGCTTTTGAGTATAAAGAAGTATTCCCCGAAAATGGGTGGAAACTGTATGACCCCCTT
  CTAGAGTACAGAAGGCAGGGAATTCCAAATGAAAGCTGGAGAATAACAAAGATAAATGAACGCTATGAAC
  TCTGTGACACATATCCTGCCCTCTTGGTTGTGCCAGCAAACATTCCTGATGAGGAGCTAAAGAGAGTGGC
  ATCCTTTAGGTCACGGGGCCGGATCCCAGTTTTATCCTGGATTCATCCTGAAAGTCAAGCCACAGTCACT
  CGCTGCAGCCAGCCCATGGTTGGAGTGAGTGGGAAGCGCAGCAAAGAAGATGAGAAGTACCTTCAGGCCA
  TCATGGACTCCAATGCCCAGTCTCACAAAATATTTATCTTTGATGCTCGGCCAAGTGTCAATGCGGTTGC
  CAATAAGGCAAAGGGGGGAGGTTATGAAAGTGAAGATGCCTATCAAAATGCAGAACTCGTTTTTCTGGAT
  ATTCATAATATCCATGTTATGAGAGAATCATTACGGAAGCTTAAGGAGATTGTGTACCCCACCATTGAAG
  AAACTCACTGGTTGTCTAACTTAGAATCTACACACTGGCTGGAACACATTAAGCTCATTCTTGCAGGGGC
  TCTTAGGATTGCTGACAAGGTCGAGTCTGGGAAGACGTCTGTGGTGGTACACTGCAGTGATGGATGGGAT
  CGCACAGCTCAGCTCACTTCCCTGGCCATGCTCATGTTGGATGGATACTACCGAACCATACGAGGATTTG
  AAGTCCTTGTGGAGAAAGAATGGCTGAGCTTTGGACATAGATTCCAACTAAGAGTTGGCCATGGAGATAA
  GAACCATGCTGACGCAGACAGGTCACCTGTTTTCCTTCAGTTTATTGACTGTGTCTGGCAAATGACAAGA
  CAGTTCCCTACCGCATTTGAATTCAATGAGTATTTTCTCATTACCATCCTGGACCACTTGTACAGCTGTT
  TATTTGGAACATTCCTTTGTAACAGCGAACAGCAGAGGGGGAAAGAGAATCTTCCTAAGAAGACAGTGTC
  ACTGTGGTCCTATATAAACAGCCAGCTGGAAGACTTTACTAATCCTCTTTATGGAAGCTACTCCAATCAT
  GTCCTTTACCCAGTAGCCAGCATGCGCCACCTAGAGCTCTGGGTGGGATATTATATAAGGTGGAACCCCC
  GGATGAAACCTCAGGAACCTATCCACAGCAGATACAAAGAACTTCTTGCCAAAAGAGCAGAGCTTCAAAG
  AAAAGTAGAGGAACTTCAGAGAGAAATTTCCAACCGCTCAACCTCCTCTTCAGAGAGAGCCAGCTCTCCT
  GCACAGTGTGTGACTCCTGTCCAAACTGTTGTA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG208990 representing BC063050
  Red=Cloning site Green=Tags(s)
  
  MEEPALLPGENIKDMAKDVTYICPFTGAVRGTLTVTSYRLYFKSMERDPPFVLDASLGVISRVEKIGGAS
  SRGENSYGLETVCKDIRNLRFAHKPEGRTRRSIFENLMKYAFPVSNGLPLFAFEYKEVFPENGWKLYDPL
  LEYRRQGIPNESWRITKINERYELCDTYPALLVVPANIPDEELKRVASFRSRGRIPVLSWIHPESQATVT
  RCSQPMVGVSGKRSKEDEKYLQAIMDSNAQSHKIFIFDARPSVNAVANKAKGGGYESEDAYQNAELVFLD
  IHNIHVMRESLRKLKEIVYPTIEETHWLSNLESTHWLEHIKLILAGALRIADKVESGKTSVVVHCSDGWD
  RTAQLTSLAMLMLDGYYRTIRGFEVLVEKEWLSFGHRFQLRVGHGDKNHADADRSPVFLQFIDCVWQMTR
  QFPTAFEFNEYFLITILDHLYSCLFGTFLCNSEQQRGKENLPKKTVSLWSYINSQLEDFTNPLYGSYSNH
  VLYPVASMRHLELWVGYYIRWNPRMKPQEPIHSRYKELLAKRAELQRKVEELQREISNRSTSSSERASSP
  AQCVTPVQTVV
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC063050
• ORF Size: 1715 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC063050, AAH63050
• RefSeq Size: 3125 bp
• RefSeq ORF: 1715 bp
• Locus ID: 77116
• Cytogenetics: 9 A1
• Gene Summary: Phosphatase that acts on lipids with a phosphoinositol headgroup (PubMed:12045210, PubMed:16399794). Has phosphatase activity towards pho sphatidylinositol 3-phosphate and phosphatidylinositol 3,5-bisphosphate (PubMed:12045210, PubMed:16399794). Binds phosphatidylinositol 4-phosphate, phosphatidylinositol 5-phosphate, phosphatidylinositol 3,5-bisphosphate and phosphatidylinositol 3,4,5-trisphosphate (PubMed:12045210, PubMed:16399794). Stabilizes SBF2/MTMR13 at the membranes (PubMed:23297362). Specifically in peripheral nerves, stabilizes SBF2/MTMR13 protein (PubMed:23297362).[UniProtKB/Swiss-Prot Function]