Rgs3 (BC033449) Mouse Tagged ORF Clone

CAT#: MG208960

Rgs3 (tGFP-tagged) - Mouse regulator of G-protein signaling 3 (cDNA clone MGC:27997 IMAGE:3599345)

Product Images

Circular map for MG208960

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Rgs3
• Synonyms: C2pa, PDZ-RGS3, RGS3S, C2PA-RGS3
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG208960 representing BC033449
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTTGAGACAGAGGCAGATGAGAAGGAGATGCCCCTGGTCGAGGGGAAGGGGCCAGGTGCTGAGGAAC
  CCGCACCCAGCAAAAATCCCTCTCCTGGCCAGGAGCTTCCTCCAGGACAAGACCTCCCTCCCAGCAAAGA
  CCCCTCTCCCAGCCAGGAGCTTCCTGCAGGACAAGATCTCCCTCCCAGCAAAGACCCCTCTCCCAGCCAG
  GAGCTTCCTGCAGGACAAGATCTCCCTCCCAGCAAAGACCCCTCTCCCAGCCAGGAGCTTCCTGTAGGAC
  AAGACCTCCCACCCAGGAAGGACTCCTCAGGCCAAGAAGCTGCTCCTGGCCCAGAATCACCATCCAGTGA
  AGACATAGCAACCTGCCCTAAGCCCCCTCAAAGCCCAGAAACCTCAACAAGCAAGGACTCCCCACCAGGC
  CAGGGATCCTCCCCGACCACAGAACTCCCATCTTGCCAGGGCCTTCCTGCTGGTCAAGAATCTACTAGCC
  AGGACCCTCTGCTCAGTCAAGAGCCCCCTGTTATCCCAGAATCCTCTGCCTCCGTCCAGAAACGCCTACC
  CTCTCAGGAGTCACCCTCCAGCCTGGGCTCCCTGCCAGAGAAGGACCTTGCTGAGCAGACCATCAGCTCC
  GGGGAGCCACCAGTCGCCACTGGTGCTGTACTGCCAGCCTCTAGACCTAACTTTGTGATCCCCGAGGTGC
  GTCTGGATAATGCCTACAGCCAACTGGATGGGGCCCACGGAGGCAGCTCTGGCGAGGACGAGGATGCAGA
  AGAGGGAGAGGAGGGGGGAGAAGGGGAGGAAGATGAGGAGGACGACACCAGCGACGACAACTACGGGGAT
  CGTAGTGAGGCCAAGCGCAGCAGCCTGATTGAGACTGGCCAAGGCGCCGAGGGCGGCTTCTCGTTGCGTG
  TGCAGAACTCGCTGCGGCGCCGGACGCACAGCGAGGGCAGCCTGCTGCAGGAGTCCCGGGGGCCCTGCTT
  TGCCTCTGACACCACCTTACACTGCTCTGATGGCGAGGGCGCCACATCCACCTGGGCTATCCCTTCACCC
  CGCACCCTCAAAAAAGAACTGGGTCGTAATGGAGGCTCCATGCACCACCTTTCCCTGTTCTTCACGGGAC
  ACAGGAAGATGAGTGGGACTGACCTCACAGAATGTGATGAAGCTTCCCGGAAGAGAAAGAGCAAAAACAT
  AGCCAAGGACATGAAGAATAAGCTGGCCATCTTCAGGCGGCGGAACGAATCTCCCGGGGCTCAGCCAGCC
  AGCAAGACAGACAAGACAACCAAGTCCTTCAAGCCTACCTCGGAGGAAGCCCTCAAGTGGAGCGAGTCCC
  TGGAAAAGTTGCTGCTTCATAAATATGGCTTAGAAGTGTTCCAGGCCTTCCTTCGCACCGAGTTCAGTGA
  GGAGAACCTGGAGTTTTGGCTCGCTTGCGAGGACTTCAAGAAGGTCAAATCACAGTCCAAGATGGCAGCC
  AAAGCCAAGAAGATCTTTGCTGAATTCATCGCAATCCAGGCTTGCAAAGAGGTAAACCTGGACTCCTACA
  CACGAGAACACACCAAGGAGAATCTGCAGAGCATCACCCGAGGCTGCTTTGACTTGGCACAGAAACGTAT
  CTTTGGGCTCATGGAGAAGGACTCTTACCCTCGCTTCCTCCGCTCTGACCTCTACCTGGACCTCATTAAC
  CAGAAGAAGATGAGTCCCCCGCTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG208960 representing BC033449
  Red=Cloning site Green=Tags(s)
  
  MFETEADEKEMPLVEGKGPGAEEPAPSKNPSPGQELPPGQDLPPSKDPSPSQELPAGQDLPPSKDPSPSQ
  ELPAGQDLPPSKDPSPSQELPVGQDLPPRKDSSGQEAAPGPESPSSEDIATCPKPPQSPETSTSKDSPPG
  QGSSPTTELPSCQGLPAGQESTSQDPLLSQEPPVIPESSASVQKRLPSQESPSSLGSLPEKDLAEQTISS
  GEPPVATGAVLPASRPNFVIPEVRLDNAYSQLDGAHGGSSGEDEDAEEGEEGGEGEEDEEDDTSDDNYGD
  RSEAKRSSLIETGQGAEGGFSLRVQNSLRRRTHSEGSLLQESRGPCFASDTTLHCSDGEGATSTWAIPSP
  RTLKKELGRNGGSMHHLSLFFTGHRKMSGTDLTECDEASRKRKSKNIAKDMKNKLAIFRRRNESPGAQPA
  SKTDKTTKSFKPTSEEALKWSESLEKLLLHKYGLEVFQAFLRTEFSEENLEFWLACEDFKKVKSQSKMAA
  KAKKIFAEFIAIQACKEVNLDSYTREHTKENLQSITRGCFDLAQKRIFGLMEKDSYPRFLRSDLYLDLIN
  QKKMSPPL
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC033449
• ORF Size: 1704 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC033449.1
• RefSeq Size: 2821 bp
• RefSeq ORF: 1706 bp
• Locus ID: 50780
• Cytogenetics: 4 33.19 cM
• Gene Summary: Down-regulates signaling from heterotrimeric G-proteins by increasing the GTPase activity of the alpha subunits, thereby driving them into their inactive GDP-bound form. Down-regulates G-protein-mediated release of inositol phosphates and activation of MAP kinases.[UniProtKB/Swiss-Prot Function]