Ggt1 (NM_008116) Mouse Tagged ORF Clone

CAT#: MG208958

Ggt1 (tGFP-tagged) - Mouse gamma-glutamyltransferase 1 (Ggt1)

Product Images

Circular map for MG208958

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Ggt1
• Synonyms: CD224; dwg; GGT; GGT-1; GGT 1; Ggtp
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG208958 representing NM_008116
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGAATCGGTTTCTGGTGCTGGGCCTGGTGGCTGTGGTTCTGGTATTTGTCATCATCGGCCTCTGTA
  TCTGGTTGCCCTACACCTCTGGGAAGCCCGACCACGTGTACTCCAGGGCGGCGGTGGCCACCGACGCCAA
  GCGCTGCTCAGAGATTGGACGGGATATCCTACAGGAAGGTGGTTCCGTGGTGGATGCAGCCATCGCAAGC
  CTGCTGTGTATGGGGCTCATGAATGCCCACAGTATGGGCATCGGGGGCGGCCTGTTCTTCACCATCTACA
  ACAGCACCACAGGAAAAGTTGAGGTTATCAATGCCCGTGAGGTAGCTCCCAGGTTGGCCAATACCACCAT
  GTTCAACAACTCCAAGGACTCTGAAGAAGGTGGCCTTTCAGTGGCTGTCCCTGGTGAAATCCGTGGTTAT
  GAGCTGGCACACCAACGACACGGCCGGCTCCCCTGGGCTCGCCTCTTCCAACCCAGCATCCAGCTGGCTC
  GCCACGGCTTCCCTGTGGGCAAGGGTTTGGCAATAGCCCTGGACAAAAAACGGGACGTCATTGAGAAGAC
  CCCTGCCTTGTGCGAGGTGTTCTGCCGCCAAGGGAAGGTGCTTCAAGAAGGAGAGACGGTGACTATGCCG
  AAGCTGGCTGACACATTGCAGATACTGGCCCAGGAGGGCGCCAAGGCCTTCTACAATGGGAGCCTCACAG
  CCCAGATTGTGAAAGACATCCAGGAGGCTGGTGGCATTATGACGGTTGAGGACCTCAACAACTACCGTGC
  TGAGCTGATTGAGCATCCGATGAGCATCGGCCTCGGTGATGCGACCCTGTACGTGCCCAGTGCCCCACTC
  AGCGGGCCCGTGCTGATTCTCATCTTGAACATCCTCAAAGGATACAACTTCTCTCCGAAGAGCGTAGCGA
  CCCCTGAACAGAAGGCACTGACGTATCACCGTATCGTGGAGGCCTTTCGGTTTGCCTATGCCAAGAGGAC
  CATGCTCGGTGACCCAAAGTTTGTCGATGTGTCTCAGGTGATCCGCAACATGAGCTCTGAGTTCTACGCC
  ACCCAGCTCCGAGCCCGCATCACTGATGAAACCACCCACCCAGCCGCCTACTATGAGCCTGAATTCTACC
  TTCAGGATGATGGAGGCACCGCTCACCTGTCTGCGGTTTCAGAGGATGGCAGTGCTGTGGCTGCCACTAG
  TACCATCAACCTCTACTTTGGCTCCAAGGTCCTGTCTCGGGTCAGTGGCATCCTGTTTAATGATGAGATG
  GATGACTTCAGCTCGCCCAACTTCATCAACCAGTTCAGGGTAGCCCCCTCACCAGCCAACTTCATCAAGC
  CAGGTAAGCAGCCTCTTTCATCCATGTGTCCCTCAATCATCCTGGATAAGGACGGCCAGGTTCGCATGGT
  GGTCGGAGCCTCAGGGGGTACCCAGATCACCACATCTGTTGCACTGGCCATCATCAACAGCCTGTGGTTC
  GGATACGATGTGAAGAGAGCTGTGGAGGAGCCCCGGCTGCACAACCAGCTTTTACCTAATACCACAACGG
  TAGAGAAAGACATTGATCAGGTGGTGACTGCAGGCCTGAAGATTCGGCACCACCATACAGAAGTCACGCC
  CACCTTCATCGCTGTAGTTCAGGCTGTCGTTCGAGCATCAGGTGGTTGGGCAGCTGCCTCAGACTCCAGA
  AAAGGCGGGGAACCTGCTGGCTAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG208958 representing NM_008116
  Red=Cloning site Green=Tags(s)
  
  MKNRFLVLGLVAVVLVFVIIGLCIWLPYTSGKPDHVYSRAAVATDAKRCSEIGRDILQEGGSVVDAAIAS
  LLCMGLMNAHSMGIGGGLFFTIYNSTTGKVEVINAREVAPRLANTTMFNNSKDSEEGGLSVAVPGEIRGY
  ELAHQRHGRLPWARLFQPSIQLARHGFPVGKGLAIALDKKRDVIEKTPALCEVFCRQGKVLQEGETVTMP
  KLADTLQILAQEGAKAFYNGSLTAQIVKDIQEAGGIMTVEDLNNYRAELIEHPMSIGLGDATLYVPSAPL
  SGPVLILILNILKGYNFSPKSVATPEQKALTYHRIVEAFRFAYAKRTMLGDPKFVDVSQVIRNMSSEFYA
  TQLRARITDETTHPAAYYEPEFYLQDDGGTAHLSAVSEDGSAVAATSTINLYFGSKVLSRVSGILFNDEM
  DDFSSPNFINQFRVAPSPANFIKPGKQPLSSMCPSIILDKDGQVRMVVGASGGTQITTSVALAIINSLWF
  GYDVKRAVEEPRLHNQLLPNTTTVEKDIDQVVTAGLKIRHHHTEVTPTFIAVVQAVVRASGGWAAASDSR
  KGGEPAGY
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_008116
• ORF Size: 1704 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_008116.3
• RefSeq Size: 2022 bp
• RefSeq ORF: 1707 bp
• Locus ID: 14598
• UniProt ID: Q60928
• Cytogenetics: 10 C1
• Gene Summary: This gene encodes gamma-glutamyl transpeptidase, a plasmamembrane-associated enzyme that cleaves the peptide bond between gamma-glutamyl and cysteinyl glycine moieties of glutathione. The encoded protein is autocatalytically processed to generate an enzymatically active heterodimer comprised of heavy and light chains. Mice lacking the encoded protein grow slowly, develop cataracts and have elevated levels of glutathione in plasma and urine. Transgenic overexpression of the encoded protein in mice enhances osteoclastic bone resorption. The mutant alleles termed 'Dwarf grey' and 'Dwarf grey Bayer' in mice are associated with deletions in this gene. A gamma-glutamyl transpeptidase paralog is located adjacent to this gene. Alternative splicing results in multiple transcript variants. Additional transcripts using alternate promoters and differing in 5' UTRs have been described. [provided by RefSeq, Apr 2015]