Chst15 (NM_029935) Mouse Tagged ORF Clone

CAT#: MG208889

Chst15 (tGFP-tagged) - Mouse RIKEN cDNA 4631426J05 gene (4631426J05Rik)

Product Images

Circular map for MG208889

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Chst15
• Synonyms: 4631426J05Rik; BRAG; GalNAcS-6ST; MAd5; mKIAA0598
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG208889 representing NM_029935
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGGCATTGCATTAATTGCTGCGTCCAGCTGTTCCCCGAAGACACACACAAACAGCAAGTTGCCTGCC
  AAGGAGGCCCCCATCACAGTCATCAGGCGTGCCCCACTTGCAAAGGAGAAAACAAAATTCTGTTTCGTGT
  GGACAGTAAGCAGATGAACTTGCTTGCTGTTCTCGAAGTGAGGACTGAGGGCAATGAAAACTGGGGTGGG
  TTTTTGCGCTTCAGGAAGGGGAAGCGATGTAGCCTGGTCTTTGGATTGATAATAATGACCTTGGTGATGG
  CTTCTTACATCCTTTCCGGGGCTCATCAGGAACTTCTGATTTCCTCCCCTTTCCATTATGGGGGCTTTCC
  CAGCAACCCCAGCGTGATGGATGGTGAGAACCCCAGTGATGTGAAAGAGCATCACTACCAGCCTTCTGTA
  AATAACATCTCCTATGTAAAGGACTATCCGAGCATTAAACTGATTATCGACAGCATTGCTGCCAGGATTG
  AGTTCACAACCAGGCAGCTCCCGGACTTACAAGATCTCAAGAGACAAGAGTTGCATATGTTTTCTGTAAT
  CCCCAGCAAATTCCTCCCGACTAGCAAGAGCCCTTGTTGGTATGAGGAGTTCTCGGGCAGGAACACCACT
  GACCCCTACCTGACCAATTCCTACGTGCTCTACTCCAAGCGGTTCCGCTCTACCTTTGACGCTCTGCGCA
  AGGTTTTCTGGGGCCACCTGTCCCACGTGCAGGGCAAGCACTTCCGCCTGCGCTGCCTGCCCCACTTCTA
  CATCATTGGGCAGCCCAAGTGTGGAACCACTGACCTGTACGACCGCCTCCGGCTGCATCCAGAAGTGAAA
  TTCTCAGCCATCAAGGAGCCGCACTGGTGGACCCGGAAGCGCTTTGGAATTGTCCGCCTGCGGGACGGAC
  TACGAGACCGCTACCCTGTGGAAGATTACCTGGACCTCTTTGACTTGGCTGCACACCAGATCCATCAAGG
  ACTGCAGGCTGCCTCTGCAGAGCAGCCCAGCAAGATGAATAAGATCATTATTGGAGAGGCCAGCGCCTCT
  ACAATGTGGGATAACAATGCCTGGACCTTCTTCTATGACAACAGCACAGACGGCGAGCCACCATTCCTGA
  CCCAAGACTTCATCCATGCCTTTCAACCAGAAGCCAAGCTCATTGTTATGCTCAGGGATCCTGTGGAGAG
  GTTGTACTCAGACTATCTCTACTTCGCAAGTTCCAATAAATCTGCAGATGACTTCCACGAGAAAGTGACG
  GAGGCTCTGCAGCTGTTTGAAAATTGCATGCTGGATTATTCACTGCGCGCCTGCGTCTACAACAACACCC
  TGAACAATGCCATGCCCGTGAGGCTCCAGGTTGGCCTGTATGCTGTGTACCTCCTGGATTGGCTGACTGT
  CTTCAGTAAGGAGCAGTTCCTCATTCTCCGTCTGGAAGACCACGCATCCAATGTCAAGTATACCATGCAC
  AAGGTCTTCCAGTTTCTAAACCTGGGGCCTCTGAGTGAGAAGCAGGAAGCTCTGATGACCAAGAGCCCTG
  CATCCAACACACGGCGTCCTGAGGATCGTAGTCTGGGACCCATGTGGCCCATCACCCAGAAGATTCTGCG
  TGAATTCTATGGGCCTTTCAACACAAGGCTGGCACAGGTCTTGGATGACGAAGCATTTGCCTGGAAGACA
  ACG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG208889 representing NM_029935
  Red=Cloning site Green=Tags(s)
  
  MRHCINCCVQLFPEDTHKQQVACQGGPHHSHQACPTCKGENKILFRVDSKQMNLLAVLEVRTEGNENWGG
  FLRFRKGKRCSLVFGLIIMTLVMASYILSGAHQELLISSPFHYGGFPSNPSVMDGENPSDVKEHHYQPSV
  NNISYVKDYPSIKLIIDSIAARIEFTTRQLPDLQDLKRQELHMFSVIPSKFLPTSKSPCWYEEFSGRNTT
  DPYLTNSYVLYSKRFRSTFDALRKVFWGHLSHVQGKHFRLRCLPHFYIIGQPKCGTTDLYDRLRLHPEVK
  FSAIKEPHWWTRKRFGIVRLRDGLRDRYPVEDYLDLFDLAAHQIHQGLQAASAEQPSKMNKIIIGEASAS
  TMWDNNAWTFFYDNSTDGEPPFLTQDFIHAFQPEAKLIVMLRDPVERLYSDYLYFASSNKSADDFHEKVT
  EALQLFENCMLDYSLRACVYNNTLNNAMPVRLQVGLYAVYLLDWLTVFSKEQFLILRLEDHASNVKYTMH
  KVFQFLNLGPLSEKQEALMTKSPASNTRRPEDRSLGPMWPITQKILREFYGPFNTRLAQVLDDEAFAWKT
  T
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_029935
• ORF Size: 1683 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_029935.6
• RefSeq Size: 4264 bp
• RefSeq ORF: 1686 bp
• Locus ID: 77590
• UniProt ID: Q91XQ5
• Cytogenetics: 7 F3
• Gene Summary: Sulfotransferase that transfers sulfate from 3'-phosphoadenosine 5'-phosphosulfate (PAPS) to the C-6 hydroxyl group of the GalNAc 4-sulfate residue of chondroitin sulfate A and forms chondroitin sulfate E containing GlcA-GalNAc(4,6-SO(4)) repeating units. It also transfers sulfate to a unique non-reducing terminal sequence, GalNAc(4SO4)-GlcA(2SO4)-GalNAc(6SO4), to yield a highly sulfated structure similar to the structure found in thrombomodulin chondroitin sulfate. May also act as a B-cell receptor involved in BCR ligation-mediated early activation that mediate regulatory signals key to B-cell development and/or regulation of B-cell-specific RAG expression; however such results are unclear in vivo (By similarity).[UniProtKB/Swiss-Prot Function]