Dtx1 (BC053055) Mouse Tagged ORF Clone

CAT#: MG208731

Dtx1 (tGFP-tagged) - Mouse deltex 1 homolog (Drosophila) (cDNA clone MGC:62339 IMAGE:5685525)

Product Images

Circular map for MG208731

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Dtx1
• Synonyms: Fxit1; mKIAA4160
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG208731 representing BC053055
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCATCAGTTCCGGCAAGACACAGGTACCATGCGGCCGGTGCGACGCAACTTCTACGATCCATCGTCGG
  CGCCGGGCAAGGGCATCGTGTGGGAATGGGAGAACGACGGCGGGGCGTGGACGGCCTACGACATGGACAT
  CTGCATCACCATCCAGAACGCGTACGAGAAGCAGCACCCGTGGCTCGACCTCTCATCGCTCGGCTTCTGC
  TACCTCATCTACTTCAACAGCATGTCCCAGATGAACCGCCAGACGCGCCGCCGCCGCCGCCTGCGCCGTC
  GCCTGGACCTGGCTTACCCGCTCACTGTCGGCTCCATTCCCAAGTCGCAATCCTGGCCCGTGGGAGCCAG
  CTCGGGTCAGCCCTGCTCCTGTCAGCAGTGCCTGCTGGTCAACAGCACGCGCGCCGCCTCCAACGCCATC
  CTGGCCTCGCAGCGCCGCAAGGCTCCCATTGCGCCAGCCGCGCCTCCAGCGCCCCCTCCGCCCCCGCCGC
  CGCTGCCACCCGGAGGACCTCCGGGTGCGCTCGTTGTGCGCCCCAGCGCCACTTTCGCCGGAGCTGCGCT
  CTGGGCCGCACCTGCCACCGGCCCCACGGAGCCTGCGCCGCCTCCAGGAGTTCCCCCAAGGAGCCCTAGT
  GCCCCCAACGGAGCGCCCACACCGGGCCAAAACAACCTCAGTCGACCAGGACCACAGAGGTCCACCAGCG
  TCAGCGCACGCGCCTCTATCCCGCCTGGGGTTCCGGCGCTCCCCGTGAAGAACTTGAATGGCACTGGCCC
  TGTCCACCCAGCCTTGGCAGGGATGACCGGGATCCTGCTGTGTGCAGCGGGGCTGCCGGTGTGCCTGACA
  CGAGCACCCAAACCCATCCTGCACCCACCACCAGTAAGCAAAAGCGACGTGAAGCCTGTGCCTGGAGTGC
  CCGGCGTGTGCCGCAAGACCAAGAAGAAACACCTCAAGAAAAGCAAGAATCCTGAGGATGTGGTTCGGAG
  GTACATGCAGAAGGTGAAAAACCCGCCTGATGAGGACTGTACCATTTGCATGGAGCGGCTGGTCACAGCA
  TCTGGCTATGAGGGCGTGCTCCGAAACAAGAGTGTGCGGCCCGAGCTTGTGGGCCGCCTGGGCCGCTGCG
  GCCACATGTATCACCTGCTCTGCCTGGTGGCCATGTACTCCAATGGCAACAAGGATGGCAGCCTGCAGTG
  TCCAACCTGCAAAGCCATCTACGGGGAGAAGACAGGGACACAGCCACCAGGGAAGATGGAGTTTCACCTC
  ATCCCGCACTCGCTGCCTGGTTTTGCAGACACCCAGACGATCCGCATCGTCTATGACATCCCCACGGGCA
  TCCAGGGCCCTGAACATCCCAACCCAGGCAAGAAGTTCACAGCCAGAGGCTTCCCTCGCCACTGCTACCT
  ACCCAACAATGAGAAGGGCCGAAAGGTGCTGAGATTGCTCATCACCGCCTGGGAACGCAGACTCATCTTC
  ACTATCGGAACATCCAACACCACGGGCGAGTCGGACACCGTGGTGTGGAACGAGATTCACCACAAGACGG
  AGTTTGGTTCCAACCTCACTGGTCACGGCTACCCCGACGCCAGCTACCTAGACAACGTGCTGGCTGAGCT
  CACCGCCCAGGGGGTTTCTGAGGCCATGGCCAAGGCC
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG208731 representing BC053055
  Red=Cloning site Green=Tags(s)
  
  MHQFRQDTGTMRPVRRNFYDPSSAPGKGIVWEWENDGGAWTAYDMDICITIQNAYEKQHPWLDLSSLGFC
  YLIYFNSMSQMNRQTRRRRRLRRRLDLAYPLTVGSIPKSQSWPVGASSGQPCSCQQCLLVNSTRAASNAI
  LASQRRKAPIAPAAPPAPPPPPPPLPPGGPPGALVVRPSATFAGAALWAAPATGPTEPAPPPGVPPRSPS
  APNGAPTPGQNNLSRPGPQRSTSVSARASIPPGVPALPVKNLNGTGPVHPALAGMTGILLCAAGLPVCLT
  RAPKPILHPPPVSKSDVKPVPGVPGVCRKTKKKHLKKSKNPEDVVRRYMQKVKNPPDEDCTICMERLVTA
  SGYEGVLRNKSVRPELVGRLGRCGHMYHLLCLVAMYSNGNKDGSLQCPTCKAIYGEKTGTQPPGKMEFHL
  IPHSLPGFADTQTIRIVYDIPTGIQGPEHPNPGKKFTARGFPRHCYLPNNEKGRKVLRLLITAWERRLIF
  TIGTSNTTGESDTVVWNEIHHKTEFGSNLTGHGYPDASYLDNVLAELTAQGVSEAMAKA
  
  SGPTRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: BC053055
• ORF Size: 1647 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC053055.1
• RefSeq Size: 2882 bp
• RefSeq ORF: 1649 bp
• Locus ID: 14357
• Cytogenetics: 5 60.64 cM
• Gene Summary: Regulator of Notch signaling, a signaling pathway involved in cell-cell communications that regulates a broad spectrum of cell-fate determinations. Mainly acts as a positive regulator of Notch, but it also acts as a negative regulator, depending on the developmental and cell context. Mediates the antineural activity of Notch, possibly by inhibiting the transcriptional activation mediated by MATCH1. Involved in neurogenesis, lymphogenesis and myogenesis, and may also be involved in MZB (Marginal zone B) cell differentiation. Promotes B-cell development at the expense of T-cell development, suggesting that it can antagonize NOTCH1. Functions as an ubiquitin ligase protein in vivo, mediating ubiquitination and promoting degradation of MEKK1, suggesting that it may regulate the Notch pathway via some ubiquitin ligase activity.[UniProtKB/Swiss-Prot Function]