AI450540 (BC062949) Mouse Tagged ORF Clone

CAT#: MG208376

AI450540 (tGFP-tagged) - Mouse expressed sequence AI450540 (cDNA clone MGC:77957 IMAGE:6839812)

Product Images

Circular map for MG208376

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: AI450540
• Synonyms: AI450540; mKIAA1600
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG208376 representing BC062949
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTCTCCAAGCTTACGTCCATCCTGCAGCATGCCGTGGAGGCGCTCGCACCGTCTCTCCCGTTACAAG
  AAGATTTTGTTTATCACTGGAAGGCGATTACACACTACTACATAGAAACTTCAGATGATAAAGCCCCAGT
  GACCGACACAAACATCCCATCCCACCTGGAACAGATGTTAGACATCCTGGTCCAAGAAGAAAATGAGCGG
  GAATCTGGAGAGACAGGGCCATGCATGGAATACCTGCTCCATCACAAGATCTTGGAAACGTTATACACCT
  TAGGGAAAGCCGACTGCCCTCCAGGAATGAAGCAGCAGGTTTTGGTGTTCTATACCAAGCTTCTGGGACG
  AATCCGGCAGCCCCTGCTTCCACACATCAACGTACACAGGCCTGTGCAGAAATTAATTCGACTCTGTGGT
  GAGGTCCTTGCAACGCCAACGGAGAATGAAGAGATCCAGTTTCTCTGCATCGTGTGTGCAAAGCTGAAAC
  AGGATCCCTACTTGGTCAACTTTTTCCTGGAGAACAAATCAAAGTCCCTGGTGTCCAGAGGAGCTCTGAG
  TGTCATCTCAGAGGATGGCCCAAAAGGTCAGGACCCGGGGTCAGGAGACGTCAGCCAGTGCCAGCAGCCC
  CAGGAGCTGTCAGGTGCTACTGGGGTGGAGCCCACGGAGTCGGAGGAAGAGCCCCCTCACCAGATGGATG
  ACCTGTCTGCAAGCCTAGATGACCTCAATGTCACCTCACTGCCGGAGGCCTCTGCCGTCCGGCCAAACCA
  GGACTACAACTTAGTGAATTCTTTGTTAAACCTGACAAGAAGTCCTGACGGCCGGATAGCTGTGAAGGCC
  TGCGAGGGCTTGATGCTCTTGGTGAGCCTACCGGAGCCTGCGGCTGCCAAGTGCCTTGCTCAGAGCACCT
  GCCTGTGCGAGCTGCTGACCGGCAGACTCACCTCCCTGTACAAGGCTCTACCTCAGTCAGTGGACCCCTT
  AGACATTGAAACCGTGGAGGCAGTTAACTGGGGGTTGGACTCATACAGCCATAAAGAAGACGCTTCGGCG
  TTCCCGGGGAAGCGGGCCTTAATTTCATTTCTTTCCTGGTTCGATTACTGTGATCAGCTCATCAAGGAGG
  CACAAAAGACTGCTGCTGTTGCTCTCGCCAAAGCCATCCACGAAAGGTTCTTTGTTGGTGTCATGGAACC
  CCAGCTGATGCAGACCTCTGAGATGGGGATTCTGACCTCGACGGCCTTGCTGCACCGCATCGTCCGGCAA
  GTGACCTCAGACGTCCTGCTTCAGGAAATGGTGGTCTTCATCCTCGGAGAACAGAGGGAGCCAGAAACTC
  TGTCAGAAATTAGCAGACATCCTCTAAGACATAGGTTAATCGAACATTGCGATCACATATCTGATGAGAT
  CAGCATCATGACCCTGAGGATGTTTGAGCATCTCCTACAAAAGCCCAATGAACACATCCTCTACAACCTG
  GTCTTGAGAAACCTGGAAGAAAGAAACTATACGGAGAACAAACCTTCCTGCCCGGAAGACAAGGACGTGG
  TGGAGAACGGGCTGATAGCAGGCGCCGTA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG208376 representing BC062949
  Red=Cloning site Green=Tags(s)
  
  MFSKLTSILQHAVEALAPSLPLQEDFVYHWKAITHYYIETSDDKAPVTDTNIPSHLEQMLDILVQEENER
  ESGETGPCMEYLLHHKILETLYTLGKADCPPGMKQQVLVFYTKLLGRIRQPLLPHINVHRPVQKLIRLCG
  EVLATPTENEEIQFLCIVCAKLKQDPYLVNFFLENKSKSLVSRGALSVISEDGPKGQDPGSGDVSQCQQP
  QELSGATGVEPTESEEEPPHQMDDLSASLDDLNVTSLPEASAVRPNQDYNLVNSLLNLTRSPDGRIAVKA
  CEGLMLLVSLPEPAAAKCLAQSTCLCELLTGRLTSLYKALPQSVDPLDIETVEAVNWGLDSYSHKEDASA
  FPGKRALISFLSWFDYCDQLIKEAQKTAAVALAKAIHERFFVGVMEPQLMQTSEMGILTSTALLHRIVRQ
  VTSDVLLQEMVVFILGEQREPETLSEISRHPLRHRLIEHCDHISDEISIMTLRMFEHLLQKPNEHILYNL
  VLRNLEERNYTENKPSCPEDKDVVENGLIAGAV
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC062949
• ORF Size: 1571 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC062949, AAH62949
• RefSeq Size: 3797 bp
• RefSeq ORF: 1571 bp
• Locus ID: 226252
• Cytogenetics: 19 D2
• Gene Summary: May be required for proper functioning of the nervous system.[UniProtKB/Swiss-Prot Function]