Ccdc144b (NM_178418) Mouse Tagged ORF Clone

CAT#: MG208327

Ccdc144b (tGFP-tagged) - Mouse cDNA sequence BC050811 (BC050811)

Product Images

Circular map for MG208327

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Ccdc144b
• Synonyms: MGC58791
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG208327 representing NM_178418
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAGATGTTGGTGACTTAACTCAGTCATCGGAGACAGCCTCAGAGTATGGCAGGCCTCCCTGCCCTA
  ATTATGAAGCTATACTGAGGCTCATTGAACGTCTCAGCCTGGAATGCAAAGATTCTGGTAGCCTATTAAA
  AATCCAGAATGCAATTCATTCATATAAAAGGTTAATACAACTGAAAACTGGTCACTGCAAAGTACTTCTA
  GAGAAACTTCAAACAGTGGAGAATGAAGTCAACGGACTCCGTAAGAAGCTTGCAGACGCAGCACGTGAAC
  AACTCCAACAAGGACAAGAACTCTGCAATGTAAGGTTTACTTTAAAACAAGAAGAAGAGAAGAGAAAAAA
  TGTCGTCTGGGTTTATGACAAAATGAAGGAGCAGCTGAGAGAAAAAGAAGATCAATATAACAAAGAAGTT
  AAAATGAAACAAAAACTTGAAATTCGTGTTAGGGAACTGGATATGGACCTGAAGACTGTGAGACATAATC
  TTAACACAGTTTTAGAGGAAAGAAACTGCATCGAGAGACAACTTTTTCAAGAACAGAACACCAGAATCAT
  ACAGGATGAGATTCTGGCAAATCACCTTCACAAACAAAAGGAGCTAAAAATGGCACAAGAAAAAATTAGC
  TCTCAGTTACAGGAAGCACCGGACCAACACACCCAGTGCACGGAGAAGATGCGGGACTGCATACAAAAGC
  TTGGACTTGAAAATCACAAGTTAAAAGAGACAGCAAGAAAGCAAGCAGAGAAAATTGAACAGTTAAAGAA
  AAATCTACTAAGTGGAGTTTTGAGTGATGATCTTACTGAAAAATTGGAATCTGCATCTGCCAAATATCTA
  CATCTCTATGAACAAAATCAGCTGCTTCGGCGGGAGTTACTGTCTATGAGAACAATACAAAGGAAATGTG
  AAAAACTAAAGAAGCATAAAAAGAAGTTAGAACAAGAAGTAGTTAGCCTTAGAAGTCACATGAAAAGGAA
  TATGATAGAGTGTGGCCAAATAGAACAGTATAAATGGGAAATCGAAGAGAAGACAAAGCAGGAATTGGTG
  GGAAAGTTAAAACAAGTCAACCTATTCTTGCAGACACAAGCAGCGTATGAAGACAAGCTAGAGAAGTTAA
  GACAGAAACAGAACGCTTCAGTGCGAACTCAGATGGAGCTCAGAATAAAGGACCTGGAGTCTGAATTCTC
  TAAGATGAAAAGTCAAGTTGATTGTAATCAAATAGAAATGGAAAACTATAAGCAGCTCTACCTAGCGGAA
  GTGAAAATTAGAAAATCATTGTCAAATAAATTAAGCAAAACAGATGAAAGACTGGCAGAGGTCAAAACCA
  AACTTCTCCTGGAGAGAAAACAGTACCGAGCTTCCCAATACAGCATGGTGGACACAAGGCCTGCCCTGGA
  GCCCACTTGTCCTAGAGACAGTAATAAATTTTTCATTCCAAGAAGTTTCTCAGACGAAATCATGCTTCCT
  AAGTCAGACCTCTGGCACTCAATTGAGACCATGGAAAATTGCAAGACCAAGGCTTCTGACCAGTTGTGCA
  TCTGTCTCTCATCTCTCCAT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG208327 representing NM_178418
  Red=Cloning site Green=Tags(s)
  
  MEDVGDLTQSSETASEYGRPPCPNYEAILRLIERLSLECKDSGSLLKIQNAIHSYKRLIQLKTGHCKVLL
  EKLQTVENEVNGLRKKLADAAREQLQQGQELCNVRFTLKQEEEKRKNVVWVYDKMKEQLREKEDQYNKEV
  KMKQKLEIRVRELDMDLKTVRHNLNTVLEERNCIERQLFQEQNTRIIQDEILANHLHKQKELKMAQEKIS
  SQLQEAPDQHTQCTEKMRDCIQKLGLENHKLKETARKQAEKIEQLKKNLLSGVLSDDLTEKLESASAKYL
  HLYEQNQLLRRELLSMRTIQRKCEKLKKHKKKLEQEVVSLRSHMKRNMIECGQIEQYKWEIEEKTKQELV
  GKLKQVNLFLQTQAAYEDKLEKLRQKQNASVRTQMELRIKDLESEFSKMKSQVDCNQIEMENYKQLYLAE
  VKIRKSLSNKLSKTDERLAEVKTKLLLERKQYRASQYSMVDTRPALEPTCPRDSNKFFIPRSFSDEIMLP
  KSDLWHSIETMENCKTKASDQLCICLSSLH
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_178418
• ORF Size: 1560 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_178418.3
• RefSeq Size: 2506 bp
• RefSeq ORF: 1563 bp
• Locus ID: 241943
• Cytogenetics: 3 B