Ppat (BC023841) Mouse Tagged ORF Clone

CAT#: MG208290

Ppat (tGFP-tagged) - Mouse phosphoribosyl pyrophosphate amidotransferase (cDNA clone MGC:38417 IMAGE:5346105)

Product Images

Circular map for MG208290

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Ppat
• Synonyms: MGC38417
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG208290 representing BC023841
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCTGGAGGAGTTGGGGATCCGCGAGGAATGTGGTGTGTTTGGGTGCATCGCTTCTGGAGACTGGC
  CCACGCAGCTAGATGTGCCGCATGTGATCACTCTGGGACTCGTGGGGCTACAGCACCGAGGTCAGGAGAG
  CGCTGGTATTGTGACCAGTGATGGGAGTGCAGTGCCTAAATTCAGGGTGCATAAGGGAATGGGTCTTGTA
  AACCATGTTTTTACCGAAGACAATTTGAAGAAATTGTATGACTCAAACCTTGGAATCGGACATACGAGGT
  ACGCCACCACGGGAAAGTGTGAGCTGGAAAACTGCCAGCCATTCGTAGTTGAGACGCTTCACGGAAAGAT
  AGCTGTGGCCCATAACGGGGAACTGGTGAACGCCGCTCGGTTGCGGAAGAAGCTTCTGCGGCAGGGTATT
  GGGCTTTCCACGTCCTCTGATAGTGAAATGATTACCCAGTTACTCGCATACACCCCTCCTCAAGAAAAAG
  ATGATGCTCCAGACTGGGTAGCAAGAATTAAGAACTTAATGAAAGAAGCGCCCGCAGCATACTCCCTGGT
  TATAATGCACAGGGACTTCATTTACGCGGTGCGAGATCCATATGGAAATCGTCCTCTCTGCATCGGGCGT
  CTTATGCCAGTCTCTGATGTAAATGACAAAGAGAAAAAATCCTCAGAAACTGAAGGATGGGTGGTGTCCT
  CAGAGTCTTGCAGCTTTTTATCCATCGGTGCCAGATATTGCCATGAAGTCAAACCCGGAGAAATTGTAGA
  AATATCCAGACATGGTATCAGAACTCTTGATATAATTCCAAGGTCTAACGGAGACCCAGTGGCTTTTTGT
  ATTTTTGAATATGTTTATTTTGCAAGACCAGATAGTATGTTTGAAGACCAAATGGTTTATACAGTAAGGT
  ACCGGTGTGGTCAGCAGCTGGCAATTGAAGCACCCGTGGAGGCGGACTTGGTTAGCACAGTTCCAGAGTC
  TGCTACGCCTGCAGCGCTGGGATATGCAACAAAGTGTGGGCTGCCTTATGTGGAAGTGCTGTGTAAGAAC
  CGGTATGTAGGAAGAACCTTCATTCAACCAAACATGAGGTTAAGACAACTCGGTGTTGCAAAGAAGTTTG
  GCGTGTTGTCTGACAACTTTAAAGGCAAAAGAATCGTTCTCATAGATGATTCGATTGTGAGGGGCAATAC
  TATCTCACCCATCATAAAACTCCTCAAAGAATCCGGTGCAAAAGAGGTGCACATTCGAGTCGCTTCACCA
  CCAATAAAATATCCGTGCTTCATGGGAATAAACATTCCTACAAAAGAAGAGCTCATAGCCAATAAGCCAG
  AATTTGATTGTCTTGCAGAGTACCTCGGAGCAAACAGCGTTGTGTATCTGTCAGTGGAAGGACTGGTTTC
  ATCTGTGCAACAAGAAATAAAATTTAAGAAGCAGAAAGTGAAAAAACATGATATTGCAATTCAAGAAAAT
  GGAAATGGTTTGGAATATTTTGAAAAGACGGGACACTGTACAGCTTGCCTCACTGGGCAGTACCCTGTGG
  AGCTGGAATGG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG208290 representing BC023841
  Red=Cloning site Green=Tags(s)
  
  MELEELGIREECGVFGCIASGDWPTQLDVPHVITLGLVGLQHRGQESAGIVTSDGSAVPKFRVHKGMGLV
  NHVFTEDNLKKLYDSNLGIGHTRYATTGKCELENCQPFVVETLHGKIAVAHNGELVNAARLRKKLLRQGI
  GLSTSSDSEMITQLLAYTPPQEKDDAPDWVARIKNLMKEAPAAYSLVIMHRDFIYAVRDPYGNRPLCIGR
  LMPVSDVNDKEKKSSETEGWVVSSESCSFLSIGARYCHEVKPGEIVEISRHGIRTLDIIPRSNGDPVAFC
  IFEYVYFARPDSMFEDQMVYTVRYRCGQQLAIEAPVEADLVSTVPESATPAALGYATKCGLPYVEVLCKN
  RYVGRTFIQPNMRLRQLGVAKKFGVLSDNFKGKRIVLIDDSIVRGNTISPIIKLLKESGAKEVHIRVASP
  PIKYPCFMGINIPTKEELIANKPEFDCLAEYLGANSVVYLSVEGLVSSVQQEIKFKKQKVKKHDIAIQEN
  GNGLEYFEKTGHCTACLTGQYPVELEW
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC023841
• ORF Size: 1553 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC023841, AAH23841
• RefSeq Size: 1958 bp
• RefSeq ORF: 1553 bp
• Locus ID: 231327
• Cytogenetics: 5 C3.3