Pla2g3 (BC079556) Mouse Tagged ORF Clone

CAT#: MG208107

Pla2g3 (tGFP-tagged) - Mouse phospholipase A2, group III (cDNA clone MGC:90545 IMAGE:30360278)

Product Images

Circular map for MG208107

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Pla2g3
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG208107 representing BC079556
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGGTCCTGGGGGTACTTCTAGGAGTGCTGGCTTTCCTAGAGGGCTCCCATACCCGTCACTGGGACA
  GTACCTCCTGCCATTTGGTCCAGCCCATCCCTGGTAACCCTCTGGGCTCCCTGAGCTTCTTGGGCAAAGA
  TGCCCAAGGCCTGGCATTATTCCAGGCCTTCTGGGATACACACCACAGGCTGCAGGTTTGTATCAGGCAG
  GATGAATCTGAACTTATCACAGCGTTCAGGGCCCTCTGTGCTCATGAGCCTTTGCAGCACTCCTTCATCC
  AAACCCCTGGACCTGCGCTGCAGAGAGCACTGGCCACTCTGCAGAGCCAGTGGGAGGCATGTCAAAGGTC
  TCAGGATAGTCCTACAGGAGCCAGGGAGAAGCGAGCAATAGAGCAGAGTGGAGCACCTGACAGAGAGCAC
  CGTCGAAGGAGGAGAGGCTGGACCATTCCTGGCACGCTGTGGTGCGGTGTTGGGAACTCTGCTGAAAATG
  CCTCAGAACTGGGCGTGTTCCACGGCCCTGACCTCTGCTGCCGGGAACACGATCAGTGCCCACAAACCAT
  TTCGCCCTTGCAGTATAACTATGGCATCCGAAATTTCCGCTTCCACACCATCTCTCACTGCGACTGTGAC
  GTCAGGTTTCAACAGTGCCTGAGGAGCCAGGGTGACTCCATCTCCGACATCATGGGCGTGGCCTTCTTCA
  ACGTGCTGGAGATCCCCTGCTTTGTGCTGAAGGAGCAGGAGGCCTGTGTGGCTTGGAACTGGTGGGGCGG
  GTGCAGGGCATATGGCTCCACGCCCCTTGCTCACCTTCGGCCCAGGATTTACTATAATGCCTCCTGGAAG
  GCTGAGGCCACCTCATATACTCCCAGCCCCCAAAGCCCAGCACCCAGCAAACACCCACAGAAGAGGGGCC
  CACAGCAGACACAAGCCAGACGTCACAGCACAACTACCACTACACCTTTCCAGACCCCTGCCATCTCCTC
  CAGACCTGATATGATCCCCAGAGGCCAGCCAGGAGTTCCACATCTGGGCTTCCAGGATGGCCCAAAACAT
  CAAAGTGCCCACCGAGTCTGTCGAAGCCTTCGCCACCTGGACCAGTGTGAGCACCAGATCAAGCCCCAGG
  AAACCAAGTTCCACCTGCTCAACAGTGCCCAGATGCCCCTTTTCCACTGCGATTGCACCCGCCGTCTGGC
  ACGTTTCCTGAGGCTCCACAGCCCGCCTGCAGGCACCGACAAAGTTTGGGATCTCCTGGGTACCACCTGC
  TTTAAGCTGGCCCCCCAACTTGACTGTGCTGAGGGCAAAGGCTGTTCCAGAGACCACAGGGCCATTAAGG
  TGTCAGCTCGGCACTTGCAGAGGCTTCACAAGAGCCGACTCCATTTCAGGGATAAAGGCACAGGTGGGGC
  TCTGGCACAGCCTGTGGAGCCCCCAGGGAGCACCATGTCATTCTACAGCCAGTGTCTGCAAGTAACCCAG
  GCAATCTGGAGACGCAGGGGACAGAAGAAATTCTGGAGCTCA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG208107 representing BC079556
  Red=Cloning site Green=Tags(s)
  
  MGVLGVLLGVLAFLEGSHTRHWDSTSCHLVQPIPGNPLGSLSFLGKDAQGLALFQAFWDTHHRLQVCIRQ
  DESELITAFRALCAHEPLQHSFIQTPGPALQRALATLQSQWEACQRSQDSPTGAREKRAIEQSGAPDREH
  RRRRRGWTIPGTLWCGVGNSAENASELGVFHGPDLCCREHDQCPQTISPLQYNYGIRNFRFHTISHCDCD
  VRFQQCLRSQGDSISDIMGVAFFNVLEIPCFVLKEQEACVAWNWWGGCRAYGSTPLAHLRPRIYYNASWK
  AEATSYTPSPQSPAPSKHPQKRGPQQTQARRHSTTTTTPFQTPAISSRPDMIPRGQPGVPHLGFQDGPKH
  QSAHRVCRSLRHLDQCEHQIKPQETKFHLLNSAQMPLFHCDCTRRLARFLRLHSPPAGTDKVWDLLGTTC
  FKLAPQLDCAEGKGCSRDHRAIKVSARHLQRLHKSRLHFRDKGTGGALAQPVEPPGSTMSFYSQCLQVTQ
  AIWRRRGQKKFWSS
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC079556
• ORF Size: 1514 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC079556, AAH79556
• RefSeq Size: 2642 bp
• RefSeq ORF: 1514 bp
• Locus ID: 237625
• Cytogenetics: 11 A1
• Gene Summary: Secretory calcium-dependent phospholipase A2 that primarily targets extracellular phospholipids. Hydrolyzes the ester bond of the fatty acyl group attached at sn-2 position of phospholipids without apparent head group selectivity (PubMed:20424323). Contributes to phospholipid remodeling of low-density lipoprotein (LDL) and high-density lipoprotein (HDL) particles. Hydrolyzes LDL phospholipids releasing unsaturated fatty acids that regulate macrophage differentiation toward foam cells (By similarity). May act in an autocrine and paracrine manner (PubMed:23624557). Secreted by immature mast cells, acts on nearby fibroblasts upstream to PTDGS to synthesize prostaglandin D2 (PGD2), which in turn promotes mast cell maturation and degranulation via PTGDR (PubMed:23624557). Secreted by epididymal epithelium, acts on immature sperm cells within the duct, modulating the degree of unsaturation of the fatty acyl components of phosphatidylcholines required for acrosome assembly and sperm cell motility (PubMed:20424323). Facilitates the replacement of fatty acyl chains in phosphatidylcholines in sperm membranes from omega-6 and omega-9 to omega-3 polyunsaturated fatty acids (PUFAs) (PubMed:20424323). Coupled to lipoxygenase pathway, may process omega-6 PUFAs to generate oxygenated lipid mediators in the male reproductive tract (PubMed:20424323). At pericentrosomal preciliary compartment, negatively regulates ciliogenesis likely by regulating endocytotic recycling of ciliary membrane protein (By similarity). Coupled to cyclooxygenase pathway provides arachidonate to generate prostaglandin E2 (PGE2), a potent immunomodulatory lipid in inflammation and tumorigenesis (By similarity). At colonic epithelial barrier, preferentially hydrolyzes phospholipids having arachidonate and docosahexaenoate at sn-2 position, contributing to the generation of oxygenated metabolites involved in colonic stem cell homeostasis (PubMed:28947740). Releases C16:0 and C18:0 lysophosphatidylcholine subclasses from neuron plasma membranes and promotes neurite outgrowth and neuron survival (PubMed:17868035).[UniProtKB/Swiss-Prot Function]