Tfcp2 (NM_033476) Mouse Tagged ORF Clone

CAT#: MG208066

Tfcp2 (tGFP-tagged) - Mouse transcription factor CP2 (Tcfcp2)

Product Images

Circular map for MG208066

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Tfcp2
• Synonyms: CP-2; CP2; D230015P20Rik; LBP-1c; LBP-1d; LBP1; LSF; Tcfcp2; UBP-1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG208066 representing NM_033476
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCTGGGCTCTGAAGCTGCCGCTAGCCGACGAAGTGATCGAGTCGGGGCTTGTGCAGGACTTTGATG
  CTAGCCTGTCCGGCATTGGCCAGGAACTGGGTGCTGGTGCTTATAGTATGAGTGATGTCCTCGCATTGCC
  CATTTTTAAGCAAGAAGAGTCAAGTTTGCCTCCCGATAATGAGAACGAGATTCTGCCTTTTCAGTATGTG
  CTCTGTGCTGCCACGTCTCCAGCGGTGAAGCTCCACGACGAGACCCTGACGTACCTCAATCAAGGACAGT
  CTTATGAAATCCGAATGCTAGACAATAGAAAACTGGGAGAGCTTCCAGAACTCAACGGCAAGCTGGTGAA
  GAGCATATTCCGCGTAGTGTTCCACGACAGGCGACTGCAGTACACTGAGCACCAGCAGCTGGAGGGCTGG
  AGGTGGAACCGACCGGGAGACAGGATTCTCGACATAGATATCCCAATGTCTGTGGGTGTAATTGATCCCA
  GGGCCAATCCTACCCAGCTAAACACAGTGGAGTTCCTGTGGGACCCTTCAAAGAGGACATCTGTGTTTAT
  TCAGGTGCACTGCATCAGCACTGAGTTCACCATGAGGAAGCATGGCGGGGAGAAGGGGGTGCCGTTCCGA
  GTGCAGATCGATACCTTCAAGGAGAACGGGAACGGAGAGTACACAGAGCACTTGCACTCGGCCAGCTGCC
  AGATCAAAGTCTTCAAGCCTAAGGGTGCAGACAGAAAACAAAAAATAGATAGAGAGAAAATGGAGAAACG
  GACGCCCCATGAAAAAGAGAAATATCAACCTTCTTATGAGACAACCATACTCACCGAGTGTTCTCCGTGG
  CCTGAGATCACCTACGTCAACAATTCCCCATCCCCTGGCTTCAACAGCTCCCATAGCAGTTTCTCTCTTG
  GGGAAGGAAACGGCTCGCCCAACCACCAGCCAGAACCACCCCCTCCAGTCACGGATAACCTCTTGCCGAC
  AACCACGCCTCAGGAAGCCCAGCAATGGCTGCATCGAAACCGGTTCTCCACATTCACGAGGCTTTTCACC
  AACTTCTCAGATTTACTGAAACTAACTAGAGACGACGTGATCCAAATCTGCGGCCCTGCAGATGGAATCA
  GACTCTTTAATGCATTAAAAGGCCGGATGGTGCGGCCAAGGCTAACCATTTATGTCTGCCAGGAGTCGTT
  GCAGTTGAGGGAGCAGCAGCCGCAGCCGCAGCCGCAGCCGCAGAAGCAGGAGGATGGGGATTCAAACGGT
  ACTTTCTTCGTGTACCATGCTATCTATCTCGAGGAGCTGACAGCTGTCGAACTCACAGAAAAAATTGCTC
  AGCTTTTCAGCATTTCTCCTCACCAGATCAGCCAGATTTACAAGCAAGGGCCGACAGGGATCCATGTGGT
  CATCAGCGATGAGATGGTACAGAACTTCCAGGAAGAAGCCTGCTTTATTCTGGACACGATGGAAGCAGAA
  ACCAGTGACAGCTATCATGTCATTCTCAAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG208066 representing NM_033476
  Red=Cloning site Green=Tags(s)
  
  MAWALKLPLADEVIESGLVQDFDASLSGIGQELGAGAYSMSDVLALPIFKQEESSLPPDNENEILPFQYV
  LCAATSPAVKLHDETLTYLNQGQSYEIRMLDNRKLGELPELNGKLVKSIFRVVFHDRRLQYTEHQQLEGW
  RWNRPGDRILDIDIPMSVGVIDPRANPTQLNTVEFLWDPSKRTSVFIQVHCISTEFTMRKHGGEKGVPFR
  VQIDTFKENGNGEYTEHLHSASCQIKVFKPKGADRKQKIDREKMEKRTPHEKEKYQPSYETTILTECSPW
  PEITYVNNSPSPGFNSSHSSFSLGEGNGSPNHQPEPPPPVTDNLLPTTTPQEAQQWLHRNRFSTFTRLFT
  NFSDLLKLTRDDVIQICGPADGIRLFNALKGRMVRPRLTIYVCQESLQLREQQPQPQPQPQKQEDGDSNG
  TFFVYHAIYLEELTAVELTEKIAQLFSISPHQISQIYKQGPTGIHVVISDEMVQNFQEEACFILDTMEAE
  TSDSYHVILK
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_033476
• ORF Size: 1506 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_033476.2, NP_258437.1
• RefSeq Size: 3192 bp
• RefSeq ORF: 1509 bp
• Locus ID: 21422
• UniProt ID: Q9ERA0
• Cytogenetics: 15 56.33 cM
• Gene Summary: Binds a variety of cellular promoters including fibrinogen, alpha-globin promoters. Activation of the alpha-globin promoter in erythroid cells is via synergistic interaction with UBP1 (By similarity). Functions as part of the SSP (stage selector protein) complex. Facilitates the interaction of the gamma-globin genes with enhancer elements contained in the locus control region in fetal erythroid cells. Interacts by binding to the stage selector element (SSE) in the proximal gamma-globin promoter (By similarity).[UniProtKB/Swiss-Prot Function]