Cpq (NM_176073) Mouse Tagged ORF Clone

CAT#: MG207513

Cpq (tGFP-tagged) - Mouse plasma glutamate carboxypeptidase (Pgcp)

Product Images

Circular map for MG207513

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Cpq
• Synonyms: 1190003P12Rik; 2610034C17Rik; Hls2; Lal-1; Pgcp
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG207513 representing NM_176073
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGGTCCCTTTTCTTCCTGTTCATTGTTCACCTTTTAGCCTTGGGCTCTGGTAAAGCTGTATTCAAGA
  ATGGTGTTTCTCAGCGAACATTTCGAGAAATAAAAGAAGAAATAGCCAACTATGAAGATGTTGCTAAAGC
  AATTATCAACCTTGCTGTTTATGGTAAATACCAGAACCGGTCCTATGAGCGTTTGGGACTTCTAGTTGAT
  ACTGTTGGACCCAGACTGAGTGGCTCTAAGAACCTAGAGAAAGCTATTCAAATCATGTACCAAAACCTGC
  AGCAAGATGGGCTAGAAAATGTTCACCTGGAGCAGGTCAGAATACCCCACTGGGAGAGGGGAGAAGAATC
  TGCAGTGATGCTTGAGCCTCGAATTCACAAGATGGCTATTCTAGGTCTTGGCAGCAGCATTGGGACTCCC
  CCAGGAGGCATCACAGCAGAAGTGCTGGTGGTAGCCTCTTTTGATGAACTTCAAAGAAGAGCATCAGAAG
  CAAGAGGGAAGATCATTGTTTATAACCAGCCTTACACTGGCTATGAGAAGACTGTGCAGTACCGGGTGCA
  GGGAGCTGTGGAAGCTGCCAAGGTGGGAGCTGTGGCATCCCTCATCCAATCAGTAGCTTCCTTTTCCATC
  TACAGTCCTCACACAGGTATTCAGAAATATCAAGATGGTGTGCCCAAGATTCCAACAGCCTGTATCACAG
  TAGAAGATGCAGAAATGATGTCTCGAATGGCTTCTCGTGGGAACAAAATTGTCATTCATCTGGAAATGGG
  AGCAAAGACCTATCCAGATACTGATTCCTTCAATACTGTTGCAGAGATCACTGGGAGCATGTATCCAGAG
  GAAGTTGTCCTGGTCAGTGGACATTTGGACAGCTGGGATGTTGGGCAGGGTGCACTGGATGATGGTGGTG
  GAGCCTTCATATCATGGGAAGCACTCTCACTTGTTAAAGATCTTGGGCTGCGTCCAAAGAGGACTCTGCG
  CCTGGTGCTCTGGACTGCAGAAGAACAAGGAGGAATTGGTGCCTCCCAGTATTATGAGCTACATAAGGCA
  AATATTTCCAAGTACAGCTTGGTGATGGAAGCTGACTCAGGAACCTTCTTACCCACTGGACTGCAGTTCA
  CTGGCAGTGACAAGGCCAGGGCTATCATGAAGGAAGTCATGAATCTTCTGCAACCCCTCAATGTCACCAA
  GGTCTTTAGTAATGGAGAAGGAACTGATATTAACTTCTGGATCCAAGCTGGAGTACCTGGAGCCAGTCTG
  CGTGATGACTTGTACAAGTATTTCTTTTTCCATCATTCCCACGGAGACACCATGACTGTCATGGATCCAA
  AGCAGATGAATGTTGCTGCTGCTGTATGGGCTGTTGTCGCTTATGTTGTTGCAGACATGGATGAAATGCT
  GCCCAGGTCC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG207513 representing NM_176073
  Red=Cloning site Green=Tags(s)
  
  MRSLFFLFIVHLLALGSGKAVFKNGVSQRTFREIKEEIANYEDVAKAIINLAVYGKYQNRSYERLGLLVD
  TVGPRLSGSKNLEKAIQIMYQNLQQDGLENVHLEQVRIPHWERGEESAVMLEPRIHKMAILGLGSSIGTP
  PGGITAEVLVVASFDELQRRASEARGKIIVYNQPYTGYEKTVQYRVQGAVEAAKVGAVASLIQSVASFSI
  YSPHTGIQKYQDGVPKIPTACITVEDAEMMSRMASRGNKIVIHLEMGAKTYPDTDSFNTVAEITGSMYPE
  EVVLVSGHLDSWDVGQGALDDGGGAFISWEALSLVKDLGLRPKRTLRLVLWTAEEQGGIGASQYYELHKA
  NISKYSLVMEADSGTFLPTGLQFTGSDKARAIMKEVMNLLQPLNVTKVFSNGEGTDINFWIQAGVPGASL
  RDDLYKYFFFHHSHGDTMTVMDPKQMNVAAAVWAVVAYVVADMDEMLPRS
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_176073
• ORF Size: 1920 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_176073.4, NP_788262.1
• RefSeq Size: 1805 bp
• RefSeq ORF: 1413 bp
• Locus ID: 54381
• UniProt ID: Q9WVJ3
• Cytogenetics: 15 B3.1
• Gene Summary: Carboxypeptidase that may play an important role in the hydrolysis of circulating peptides. Catalyzes the hydrolysis of dipeptides with unsubstituted terminals into amino acids. May play a role in the liberation of thyroxine hormone from its thyroglobulin (Tg) precursor (By similarity).[UniProtKB/Swiss-Prot Function]