Tinagl (BC005738) Mouse Tagged ORF Clone

CAT#: MG207457

Tinagl (tGFP-tagged) - Mouse tubulointerstitial nephritis antigen-like (cDNA clone MGC:6860 IMAGE:2650851)

Product Images

Circular map for MG207457

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Tinagl
• Synonyms: 1110021J17Rik; Arg1; AZ-1; AZ1; Lcn7; TARP; Tinagl
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG207457 representing BC005738
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGGGGATGTTGGCTGGGGCTGCTCTTGCTGCTGCTGGCTGGCCAGGCTGCCCTGGAGGCCCGGCGGA
  GTCGTTGGCGCAGGGAGCTGGCGCCAGGGCTGCACCTGCGGGGCATCCGGGACGCCGGTGGCAGATACTG
  CCAAGAGCAGGACATGTGCTGTCGCGGCCGAGCTGACGAGTGTGCCCTGCCCTACCTGGGAGCCACCTGT
  TACTGTGACCTCTTCTGCAACCGCACCGTCTCTGACTGCTGCCCCGACTTCTGGGACTTCTGCCTCGGGA
  TTCCACCCCCCTTCCCTCCCGTCCAAGGGTGCATGCATGGGGGCCGGATCTACCCAGTCTTCGGAACCTA
  CTGGGACAACTGCAATCGATGCACCTGCCATGAGGGAGGGCATTGGGAGTGCGACCAGGAGCCGTGTCTA
  GTGGACCCAGACATGATTAAAGCCATCAACCGAGGCAACTATGGGTGGCAGGCTGGGAACCACAGTGCCT
  TCTGGGGCATGACCCTGGATGAGGGCATTCGCTACCGCCTGGGCACAATCCGCCCATCCTCCACTGTCAT
  GAATATGAATGAGATTTATACGGTGCTGGGCCAAGGGGAAGTGCTACCCACTGCCTTTGAAGCTTCAGAG
  AAGTGGCCCAACCTGATCCACGAGCCATTGGACCAGGGCAATTGTGCAGGTTCCTGGGCTTTCTCCACAG
  CAGCTGTCGCATCTGATCGCGTCTCTATCCATTCTTTGGGACACATGACACCCATCCTATCACCGCAGAA
  CCTGCTGTCCTGTGATACCCACCACCAGCAGGGCTGCCGAGGTGGGCGTCTTGATGGCGCTTGGTGGTTC
  CTGCGGCGCCGCGGGGTAGTGTCTGACAACTGTTACCCATTCTCCGGCCGTGAGCAGAACGAGGCCAGCC
  CCACTCCTCGTTGTATGATGCACAGCCGCGCCATGGGGCGGGGCAAGCGCCAAGCCACTTCCCGCTGCCC
  CAATGGTCAGGTTGATTCCAACGACATCTACCAGGTCACGCCTGCCTACCGCCTGGGCTCTGATGAGAAG
  GAGATCATGAAGGAGCTAATGGAAAACGGCCCTGTTCAAGCACTCATGGAAGTACACGAGGACTTCTTCT
  TGTACCAGCGTGGCATCTACAGCCACACACCTGTAAGCCAGGGGAGGCCGGAGCAGTACCGCAGACATGG
  GACTCACTCAGTCAAGATCACTGGGTGGGGAGAAGAGACGCTGCCAGACGGAAGGACCATCAAGTACTGG
  ACTGCTGCCAACTCGTGGGGCCCATGGTGGGGTGAGAGGGGCCACTTCCGGATCGTGCGTGGCACCAACG
  AGTGCGACATCGAGACCTTCGTGCTGGGCGTCTGGGGTCGCGTGGGAATGGAGGACATGGGGCACCAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG207457 representing BC005738
  Red=Cloning site Green=Tags(s)
  
  MWGCWLGLLLLLLAGQAALEARRSRWRRELAPGLHLRGIRDAGGRYCQEQDMCCRGRADECALPYLGATC
  YCDLFCNRTVSDCCPDFWDFCLGIPPPFPPVQGCMHGGRIYPVFGTYWDNCNRCTCHEGGHWECDQEPCL
  VDPDMIKAINRGNYGWQAGNHSAFWGMTLDEGIRYRLGTIRPSSTVMNMNEIYTVLGQGEVLPTAFEASE
  KWPNLIHEPLDQGNCAGSWAFSTAAVASDRVSIHSLGHMTPILSPQNLLSCDTHHQQGCRGGRLDGAWWF
  LRRRGVVSDNCYPFSGREQNEASPTPRCMMHSRAMGRGKRQATSRCPNGQVDSNDIYQVTPAYRLGSDEK
  EIMKELMENGPVQALMEVHEDFFLYQRGIYSHTPVSQGRPEQYRRHGTHSVKITGWGEETLPDGRTIKYW
  TAANSWGPWWGERGHFRIVRGTNECDIETFVLGVWGRVGMEDMGHH
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC005738
• ORF Size: 1400 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC005738, AAH05738
• RefSeq Size: 1967 bp
• RefSeq ORF: 1400 bp
• Locus ID: 94242
• Cytogenetics: 4 D2.2
• Gene Summary: May be implicated in the adrenocortical zonation and in mechanisms for repressing the CYP11B1 gene expression in adrenocortical cells. This is a non catalytic peptidase C1 family protein.[UniProtKB/Swiss-Prot Function]