Snx8 (NM_172277) Mouse Tagged ORF Clone

CAT#: MG207330

Snx8 (tGFP-tagged) - Mouse sorting nexin 8 (Snx8)

Product Images

Circular map for MG207330

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Snx8
• Synonyms: B130023O14Rik
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG207330 representing NM_172277
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACTGGCCGCGCGATGGACCCGCTGCCCTCGCCGGCAGTCGCTGCAGCTGCTGAGGCGGAGGCTGACG
  AGGAGGCGGATCCGCCGGCGACAGGCCCACGAACATCCCAGGTCACTGAGTGGAGGGCCCTGGACCCCGG
  GCGAATGCAGATGCCGCAGGGGAACCCACTGCTGCTGTCCTACACCCTGCAGGAGCTGCTGGCCAAGGAC
  ACGGTGCAGGTGGAGCTCATCCCTGAGAAGAAGGGCCTCTTCCTTAAGCATGTGGAGTACGAGGTTTCCA
  GCCAGCGCTTCAAGTCCTCCGTGTACAGACGCTACAATGACTTCGTGGTCTTCCATGAAGTGCTGCTTCA
  CAAGTTCCCGTACCGCATGGTTCCAGCTCTTCCGCCCAAGAGAGTGCTGGGAGCCGACAGGGAGTTCATC
  GAGGGCCGCCGGAGGGCCCTGAAGCGCTTCATCAACCTGGTGGCCCGGCACCCACCCTTCTCTGAGGACG
  TGCTCCTTAAACTCTTCCTCTCCTTCAGTGGCTCGGATGTTCAGCACAAGCTCAAGGAGGCGGCCCAGTG
  CGTGGGAGATGAGTTCATGAACTGTAAGCTGGCTGCTAGGGCCAAGGACTTCCTCCCAGCTGACATCCAG
  ACTCAATTTGCCATGAGCCGGGAGCTGATTCGAAATGTCTACAACAGCTTCTACAAGCTTCGAGACAGAG
  CCGAGCGGATTGCGTCCCGGGCCATTGACAACGCTGCTGATCTTCTCATATTTGGAAAGGAGCTTAGTGC
  TTTGGGCTCCGACACCACCCCTCTGCCCTCCTGGGCTGCTCTGCACCTCAGCACATGGGGCTCCCTGAAG
  CAAGCGCTCAAGGGCCTCTCCGTGGAGTTTGCACTGCTGGCGGACAGAGCTGCACAGCAGGGCAAGAAGG
  AGGAGAATGATGTAGTGGAGAAGCTGAACCTCTTCTTGGATTTGTTGCAGTCCTACAAAGACCTGTGTGA
  GCGGCACGAGAAGGGCGTGCTTCACAAACACCAGCGGGCACTGCACAAGTACGGCCTGATGAAGAGGCAG
  ATGATGAGTGCAGCGCACGGCCGAGAACCTGAGTCTGTGGAGCAGCTGGAGTCACGAATCGTGGAGCAGG
  AGAACGTGATCCAGACTATGGAACTCCGAAACTACTTCTCCCTCTACTGCCTGCACCAGGAGACGCAGCT
  CGTCCACGTCTACCTGCCTCTCACCTCGCACATCCTGGGGGCCTTCGTGAACTCCCAGATCCAAGGGCAC
  AAGGAGATGAGCAAGGTGTGGAATGACCTGAAGCCGAAGCTGAGCTGCCTCTTCGCTGGACCTCACAGTG
  TGTTGACGCCACCGCGCTCCCCGCAGGAGGATGGCGTGTGTCCCCAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG207330 representing NM_172277
  Red=Cloning site Green=Tags(s)
  
  MTGRAMDPLPSPAVAAAAEAEADEEADPPATGPRTSQVTEWRALDPGRMQMPQGNPLLLSYTLQELLAKD
  TVQVELIPEKKGLFLKHVEYEVSSQRFKSSVYRRYNDFVVFHEVLLHKFPYRMVPALPPKRVLGADREFI
  EGRRRALKRFINLVARHPPFSEDVLLKLFLSFSGSDVQHKLKEAAQCVGDEFMNCKLAARAKDFLPADIQ
  TQFAMSRELIRNVYNSFYKLRDRAERIASRAIDNAADLLIFGKELSALGSDTTPLPSWAALHLSTWGSLK
  QALKGLSVEFALLADRAAQQGKKEENDVVEKLNLFLDLLQSYKDLCERHEKGVLHKHQRALHKYGLMKRQ
  MMSAAHGREPESVEQLESRIVEQENVIQTMELRNYFSLYCLHQETQLVHVYLPLTSHILGAFVNSQIQGH
  KEMSKVWNDLKPKLSCLFAGPHSVLTPPRSPQEDGVCPH
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_172277
• ORF Size: 1377 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_172277.3
• RefSeq Size: 2678 bp
• RefSeq ORF: 1380 bp
• Locus ID: 231834
• UniProt ID: Q8CFD4
• Cytogenetics: 5 G2
• Gene Summary: May be involved in several stages of intracellular trafficking. May play a role in intracellular protein transport from early endosomes to the trans-Golgi network (By similarity).[UniProtKB/Swiss-Prot Function]