Cpn1 (NM_030703) Mouse Tagged ORF Clone

CAT#: MG207305

Cpn1 (tGFP-tagged) - Mouse carboxypeptidase N, polypeptide 1 (Cpn1)

Product Images

Circular map for MG207305

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Cpn1
• Synonyms: 0610011F20Rik; CPN
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG207305 representing NM_030703
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCAGACCTGCCCTCAGCCTTCCTGCCCCTCCTCCTCCTCTCCAAGTTTGTTACCCCGGTGACCTTTC
  GTCACCACCGCTACGACGACCTCGTGCGGACGCTGTACAAGGTGCACAACCAATGCCCGGACATCACGCG
  GCTCTACAACATCGGGCGCAGTGTGAAGGGGAGGTACCTCTACGTGCTGGAGTTTAGCGACTACCCTGGG
  ATCCATGAACCCTTGGAACCGGAAGTCAAGTATGTGGGAAACATGCACGGGAACGAGGTGCTGGGCCGCG
  AGCTGCTTCTGCAGCTGTCGGAATTCCTCTGCGAGGAGTTCCGGAACCGAAACCAGCGTATCCTGCGCCT
  CATTCAGGACACGCGCATTCACATCCTGCCTTCCATGAACCCTGACGGCTACGAGGTGGCTGCTGCCCAG
  GGCCCAAACATGTCTGGGTATCTGGTTGGTAGGAACAATGCAAATGGGGTGGACCTGAACCGCAACTTCC
  CGGATCTCAACACCTACTTCTACTACAACTCCAAGAACGGTGGCCCTAACCACCACCTGCCCCTCCCCGA
  CAACTGGAAAAGTCAGGTGGAACCAGAGACCCGGGCGGTGATCCAGTGGATTCGCTCCTTGAACTTCGTT
  CTCTCAGCCAATATGCACGGCGGGGCTGTGGTGGCCAATTACCCATATGACAAGTCACTTGAGCATAGGT
  TTCGAGGTCCCCATCGCACCTCCAATTCCCCGACACCTGATGACGAGCTCTTCCAGACGCTGGCCAAGGT
  CTACTCCTACGCCCATGGATGGATGCACCAAGGTTGGAACTGTGGGGACTACTTTCCAGACGGCATCACC
  AACGGGGCGTCCTGGTATTCTCTCAGCAAGGGAATGCAAGACTTCAATTATCTCCACACTAACTGCTTTG
  AGATCACGCTCGAGCTGAGTTGCGACAAGTTTCCCCGGCAAGAGGAGCTGCAGAGGGAGTGGCTGGGTAA
  CCGGGAAGCCTTAATCCAGTTCTTGGAACAGGTCCACCAGGGCATCAAGGGAATGGTGTTGGATGAGAAC
  TCTAACAACCTCACGGGGGCTGTCATTTCCGTCACCGGCATCAACCACGACGTCACTTCAGGTGAACACG
  GGGATTATTTCCGGCTGCTGCTGCTCCCGGGTACTTACTCTGTCACTGCCAAGGCACCAGGGTACGACCC
  CAAAACAGTGACTGTGACGGTGGGGCCTGCGGGACCAACAGTGGTTGACTTCCAACTCAAGCGAAGCTCA
  TCTCAGGTGTACCCTGTGCAGAGAGCTCCCGGCCGAGGGCAGGGAGGCAGGGCAAAGCAGCCCCGGACAT
  CCAGAAAGAAAGACCCAGCAACGAAACGGCATAGGGGTCCTGCA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG207305 representing NM_030703
  Red=Cloning site Green=Tags(s)
  
  MPDLPSAFLPLLLLSKFVTPVTFRHHRYDDLVRTLYKVHNQCPDITRLYNIGRSVKGRYLYVLEFSDYPG
  IHEPLEPEVKYVGNMHGNEVLGRELLLQLSEFLCEEFRNRNQRILRLIQDTRIHILPSMNPDGYEVAAAQ
  GPNMSGYLVGRNNANGVDLNRNFPDLNTYFYYNSKNGGPNHHLPLPDNWKSQVEPETRAVIQWIRSLNFV
  LSANMHGGAVVANYPYDKSLEHRFRGPHRTSNSPTPDDELFQTLAKVYSYAHGWMHQGWNCGDYFPDGIT
  NGASWYSLSKGMQDFNYLHTNCFEITLELSCDKFPRQEELQREWLGNREALIQFLEQVHQGIKGMVLDEN
  SNNLTGAVISVTGINHDVTSGEHGDYFRLLLLPGTYSVTAKAPGYDPKTVTVTVGPAGPTVVDFQLKRSS
  SQVYPVQRAPGRGQGGRAKQPRTSRKKDPATKRHRGPA
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_030703
• ORF Size: 1371 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_030703.1, NP_109628.1
• RefSeq Size: 1793 bp
• RefSeq ORF: 1374 bp
• Locus ID: 93721
• UniProt ID: Q9JJN5
• Cytogenetics: 19 36.68 cM
• Gene Summary: Protects the body from potent vasoactive and inflammatory peptides containing C-terminal Arg or Lys (such as kinins or anaphylatoxins) which are released into the circulation.[UniProtKB/Swiss-Prot Function]