Fars2 (NM_024274) Mouse Tagged ORF Clone

CAT#: MG207194

Fars2 (tGFP-tagged) - Mouse phenylalanine-tRNA synthetase 2 (mitochondrial) (Fars2), transcript variant 1

Product Images

Circular map for MG207194

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Fars2
• Synonyms: Fars1; pheRS
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG207194 representing NM_024274
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTCTGCTTGGCTCTCGTCAGAGCTGCCTATGAGCACATCTACCTGGTGAGGAAGGTCAGTCATGCCT
  GCAGATGCCATCAACATCGGGCTTGGAGCTCAAAGCCAGCTGCATCACAGTCTGCTGTCCAGGGTGCTCC
  AGGCAGTGTGCTGGAGATACTTGGTAAATCCTACCCTCAGGATGACCACACTAACCTCACCCAGAAGGTC
  CTTTCCAAGGTGGGCAGGAACCTGCACAACCAGAAGTTCCACCCTCTGTGGCTGATTAAGGAGCGGGTGA
  AGGAGCACTTCTACCAGCAGTATATGGTGCGATCCAGGACACCTCTATTCTCCGTCTATGACCAGCTTCC
  TCCAGTGGTCACCACCTGGCAGAACTTTGATAGCCTGCTAATCCCAGCTGACCACCCCAGCAGGAAAAAG
  GGGGACAACTATTACTTGAATCGGGCACACATGCTGAGAGCACACACATCAGCGCATCAGTGGGACTTGC
  TGCATGCGGGACTTAATGCCTTCCTTGTGGTAGGTGACGTGTATCGTCGTGACCAGATTGATTGCCAGCA
  CTACCCAGTTTTCCACCAGCTGGAGGGTGTCCGGCTCTTCTCCAAGCATGAGTTATTTGCTGGCGTAAAG
  GATGGAGAAAGCTTGCAGCTCTTTGAAGAAGGTTCTCGCTCTGCTCATAAGCAGGAGACACACACCATGG
  AGGCTGTGAAGCTTGTGGAGTTCGACCTTAAACAAGTACTGACTAGGCTTGTGACACATCTTTTTGGAGA
  TGGGCTGGAGGTTCGGTGGGTGGATTGCTACTTCCCCTTCACCCACCCTTCCTTCGAGATGGAGATCAAC
  TTCCGTGGAGAATGGCTTGAAGTCCTTGGCTGCGGTGTGATGGAGCAGCAGCTTGTCAATTCAGCTGGTG
  CTCAGGATCGAATCGGCTGGGCGTTTGGCCTGGGATTAGAAAGATTGGCGATGGTCCTCTACGACATTCC
  TGACATCCGCCTTTTCTGGAGTGAGGATGAGCGCTTCCTGAAGCAGTTCCTCCTGTCGGACATCAACCAG
  AGTGTCAAGTTTCAGCCTCTTAGCAAGTACCCCGCCGTGTTCAATGACATTTCATTCTGGCTGCCCTCTG
  AGAACTACACGGAGAATGATTTCTATGACATCGTCCGAACAGTCGGAGGAGACCTGGTAGAAAAGGTTGA
  TCTTATAGACAAGTTTGAACATCCAAAGACACACAGGACCAGCCACTGCTACCGCATTACCTACCGCCAC
  ATGGAACGGACACTGTCCCAGAGAGAAGTCGGCAATGTCCACCAGGCTGTGCAGGAGGCCGCCGTGCAGC
  TGCTGGGTGTGGAGGGCCGTTTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG207194 representing NM_024274
  Red=Cloning site Green=Tags(s)
  
  MVCLALVRAAYEHIYLVRKVSHACRCHQHRAWSSKPAASQSAVQGAPGSVLEILGKSYPQDDHTNLTQKV
  LSKVGRNLHNQKFHPLWLIKERVKEHFYQQYMVRSRTPLFSVYDQLPPVVTTWQNFDSLLIPADHPSRKK
  GDNYYLNRAHMLRAHTSAHQWDLLHAGLNAFLVVGDVYRRDQIDCQHYPVFHQLEGVRLFSKHELFAGVK
  DGESLQLFEEGSRSAHKQETHTMEAVKLVEFDLKQVLTRLVTHLFGDGLEVRWVDCYFPFTHPSFEMEIN
  FRGEWLEVLGCGVMEQQLVNSAGAQDRIGWAFGLGLERLAMVLYDIPDIRLFWSEDERFLKQFLLSDINQ
  SVKFQPLSKYPAVFNDISFWLPSENYTENDFYDIVRTVGGDLVEKVDLIDKFEHPKTHRTSHCYRITYRH
  MERTLSQREVGNVHQAVQEAAVQLLGVEGRF
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_024274
• ORF Size: 1893 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_024274.3, NP_077236.1
• RefSeq Size: 2140 bp
• RefSeq ORF: 1356 bp
• Locus ID: 69955
• UniProt ID: Q99M01
• Cytogenetics: 13 A3.3
• Gene Summary: Is responsible for the charging of tRNA(Phe) with phenylalanine in mitochondrial translation. To a lesser extent, also catalyzes direct attachment of m-Tyr (an oxidized version of Phe) to tRNA(Phe), thereby opening the way for delivery of the misacylated tRNA to the ribosome and incorporation of ROS-damaged amino acid into proteins (By similarity).[UniProtKB/Swiss-Prot Function]