Abi2 (NM_198127) Mouse Tagged ORF Clone

CAT#: MG207119

Abi2 (tGFP-tagged) - Mouse abl-interactor 2 (Abi2)

Product Images

Circular map for MG207119

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Abi2
• Synonyms: 8430425M24Rik; AI839867; C130078H13
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG207119 representing NM_198127
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGAGCTGCAGATGCTGCTGGAAGAGGAAATCCCGGGGGGCCGCCGGGCTCTCTTCGACAGCTACA
  CGAATCTGGAGCGGGTGGCCGACTACTGCGAGAATAACTACATCCAGTCACCAGATAAGCAGCGAGCCCT
  AGAAGAAACCAAAGCCTACACCACTCAATCCTTAGCAAGTGTTGCGTATCTGATAAACACCTTGGCCAAC
  AATGTCCTACAGATGCTGGATATCCAGGCATCACAGTTACGGAGGATGGAGTCTTCAATCAACCATATTT
  CACAAACAGTTGATATTCATAAAGAGAAGGTTGCAAGAAGAGAAATTGGTATTTTGACCACCAATAAAAA
  CACTTCAAGGACCCATAAGATTATTGCGCCAGCCAACCTTGAGCGGCCAGTTCGTTATATTCGAAAACCT
  ATTGACTACACAATTTTAGATGACATTGGACATGGAGTGAAGGTGAGTACCCAGAATATGAAAATGGGTG
  GATTGCCACGTACGACACCTCCAACTCAGAAGCCCCCAAGTCCCCCTATGTCAGGGAAGGGGACACTTGG
  GCGGCACTCCCCCTATCGAACACTGGAGCCAGTGCGTCCTCCAGTGGTACCAAATGATTACGTACCTAGC
  CCAACCCGTAATATGGCTCCCTCGCAGCAGAGCCCTGTGAGGACAGCTTCTGTGAATCAAAGAAATCGAA
  CTTACAGCAGCAGTGGGAGCAGTGGAGGAAGTCACCCAAGTAGTCGGAGCAGCAGTCGGGAGAACAGCGG
  AAGTGGTAGTGTGGGGGTACCCATTGCTGTGCCTACTCCGTCTCCTCCAAGCGTCTTTCCAGGTCATCCT
  GTTCAGTTCTACAGCATGAACAGGCCTGCCTCTCGCCATACACCACCTACAATAGGGGGCTCGTTGCCCT
  ATAGACGTCCTCCTTCCATAACGTCACAAACAAGCCTTCAGAATCAGATGAATGGAGGTCCTTTTTATAA
  CCAGAATCCAGTTTCAGATACACCACCTCCACCCCCACCTGTGGAAGAGCCAGTCTTTGATGAATCCCCC
  CCTCCACCGCCTCCTCCAGAAGATTATGAAGAGGAGGAAGCAGCTGTGGTTGAGTACAGCGATCCGTACG
  CTGAAGAGGACCCACCGTGGGCGCCAAGGGCTTACTTGGAAAAGGTTGTGGCAATTTATGATTATACAAA
  AGACAAGGAAGATGAGCTGTCCTTTCAGGAAGGAGCCATTATATATGTCATCAAGAAGAATGACGATGGT
  TGGTATGAGGGAGTTATGAATGGTGTGACCGGGCTCTTCCCTGGGAACTATGTTGAGTCCATCATGCATT
  ATTCGGAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG207119 representing NM_198127
  Red=Cloning site Green=Tags(s)
  
  MAELQMLLEEEIPGGRRALFDSYTNLERVADYCENNYIQSPDKQRALEETKAYTTQSLASVAYLINTLAN
  NVLQMLDIQASQLRRMESSINHISQTVDIHKEKVARREIGILTTNKNTSRTHKIIAPANLERPVRYIRKP
  IDYTILDDIGHGVKVSTQNMKMGGLPRTTPPTQKPPSPPMSGKGTLGRHSPYRTLEPVRPPVVPNDYVPS
  PTRNMAPSQQSPVRTASVNQRNRTYSSSGSSGGSHPSSRSSSRENSGSGSVGVPIAVPTPSPPSVFPGHP
  VQFYSMNRPASRHTPPTIGGSLPYRRPPSITSQTSLQNQMNGGPFYNQNPVSDTPPPPPPVEEPVFDESP
  PPPPPPEDYEEEEAAVVEYSDPYAEEDPPWAPRAYLEKVVAIYDYTKDKEDELSFQEGAIIYVIKKNDDG
  WYEGVMNGVTGLFPGNYVESIMHYSE
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_198127
• ORF Size: 1338 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_198127.2
• RefSeq Size: 5605 bp
• RefSeq ORF: 1341 bp
• Locus ID: 329165
• UniProt ID: P62484
• Cytogenetics: 1 C2
• Gene Summary: Regulator of actin cytoskeleton dynamics underlying cell motility and adhesion. Functions as a component of the WAVE complex, which activates actin nucleating machinery Arp2/3 to drive lamellipodia formation (By similarity). Acts as regulator and substrate of nonreceptor tyrosine kinases ABL1 and ABL2 involved in processes linked to cell growth and differentiation. Positively regulates ABL1-mediated phosphorylation of ENAH, which is required for proper polymerization of nucleated actin filaments at the leading edge (By similarity). Contributes to the regulation of actin assembly at the tips of neuron projections. In particular, controls dendritic spine morphogenesis and may promote dendritic spine specification toward large mushroom-type spines known as repositories of memory in the brain (PubMed:15572692). In hippocampal neurons, may mediate actin-dependent BDNF-NTRK2 early endocytic trafficking that triggers dendrite outgrowth (PubMed:27605705). Participates in ocular lens morphogenesis, likely by regulating lamellipodia-driven adherens junction formation at the epithelial cell-secondary lens fiber interface (PubMed:15572692). Also required for nascent adherens junction assembly in epithelial cells (By similarity).[UniProtKB/Swiss-Prot Function]