Ppp2r2b (BC026686) Mouse Tagged ORF Clone

CAT#: MG207118

Ppp2r2b (tGFP-tagged) - Mouse protein phosphatase 2 (formerly 2A), regulatory subunit B (PR 52), beta isoform (cDNA clone MGC:37708

Product Images

Circular map for MG207118

Specifications

Product Data

• Type: Mouse Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Ppp2r2b
• Synonyms: SCA12, PR55-BETA, PP2A-PR55B
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MG207118 representing BC026686
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAATGCTTCTCTCGTTACCTGCCTTATATCTTCAGACCTCCAAACACCATCCTCTCTTCCAGCTGCC
  ATACTGAAGCTGACATTATCTCTACGGTAGAATTCAACCACACGGGAGAGTTACTAGCGACGGGGGACAA
  GGGGGGTCGGGTCGTAATATTTCAGCGAGAACAGGAGAGTAAAAACCAGGTTCATCGTAGGGGTGAATAC
  AATGTTTACAGCACATTCCAGAGCCATGAACCCGAGTTCGATTACCTGAAAAGTTTAGAAATAGAAGAAA
  AAATCAATAAGATAAGATGGCTCCCTCAACAAAACGCAGCTTACTTTCTTCTGTCTACCAACGATAAAAC
  TGTGAAGCTGTGGAAAGTCAGTGAGCGGGATAAGAGGCCAGAAGGCTACAACCTGAAAGATGAGGAGGGC
  CGGCTCCGGGATCCTGCCACCATCACAACCCTGCGGGTGCCTGTCCTGAGACCCATGGACCTGATGGTGG
  AGGCCACCCCACGAAGAGTGTTTGCCAATGCGCACACGTACCACATCAACTCTATATCTGTCAACAGCGA
  CTATGAGACCTACATGTCTGCTGATGACCTGAGGATTAACCTGTGGAACTTTGAGATAACCAATCAGAGT
  TTTAATATCGTGGACATCAAGCCAGCCAACATGGAGGAGCTCACAGAGGTGATCACTGCAGCTGAGTTCC
  ACCCCCACCACTGCAACACCTTCGTATACAGCAGCAGCAAGGGGACAATCCGGCTGTGTGACATGCGTGC
  ATCTGCGCTGTGTGACAGGCACACCAAGTTTTTTGAAGAACCGGAAGATCCAAGCAACAGATCGTTTTTC
  TCTGAAATCATCTCTTCAATTTCGGATGTGAAATTCAGCCACAGTGGGAGGTATATCATGACCAGAGACT
  ATCTGACCGTGAAAGTCTGGGACCTCAACATGGAAAATCGCCCCATTGAGACATACCAGGTTCATGACTA
  CCTCCGCAGCAAGTTGTGCTCCCTCTATGAAAATGACTGCATTTTTGATAAATTTGAGTGTGTGTGGAAT
  GGGTCAGACAGCGTCATCATGACGGGCTCCTACAATAACTTCTTCAGGATGTTCGACCGAAACACCAAGC
  GTGATGTGACCCTTGAGGCCTCAAGGGAAAACAGCAAACCCCGGGCTATCCTCAAACCCCGAAAAGTGTG
  CGTCGGGGGCAAGCGAAGAAAAGATGAGATCAGTGTTGACAGTCTGGACTTTAGCAAAAAGATCTTGCAT
  ACAGCTTGGCATCCTTCAGAAAACATTATAGCAGTGGCGGCTACGAATAACTTATATATATTCCAAGACA
  AGGTTAAC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >MG207118 representing BC026686
  Red=Cloning site Green=Tags(s)
  
  MKCFSRYLPYIFRPPNTILSSSCHTEADIISTVEFNHTGELLATGDKGGRVVIFQREQESKNQVHRRGEY
  NVYSTFQSHEPEFDYLKSLEIEEKINKIRWLPQQNAAYFLLSTNDKTVKLWKVSERDKRPEGYNLKDEEG
  RLRDPATITTLRVPVLRPMDLMVEATPRRVFANAHTYHINSISVNSDYETYMSADDLRINLWNFEITNQS
  FNIVDIKPANMEELTEVITAAEFHPHHCNTFVYSSSKGTIRLCDMRASALCDRHTKFFEEPEDPSNRSFF
  SEIISSISDVKFSHSGRYIMTRDYLTVKVWDLNMENRPIETYQVHDYLRSKLCSLYENDCIFDKFECVWN
  GSDSVIMTGSYNNFFRMFDRNTKRDVTLEASRENSKPRAILKPRKVCVGGKRRKDEISVDSLDFSKKILH
  TAWHPSENIIAVAATNNLYIFQDKVN
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: BC026686
• ORF Size: 1340 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC026686, AAH26686
• RefSeq Size: 2003 bp
• RefSeq ORF: 1340 bp
• Locus ID: 72930
• Cytogenetics: 18 B3
• Gene Summary: The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment. Within the PP2A holoenzyme complex, isoform 2 is required to promote proapoptotic activity. Isoform 2 regulates neuronal survival through the mitochondrial fission and fusion balance.[UniProtKB/Swiss-Prot Function]